Lymph‐circulating tumor cells show distinct properties to blood‐circulating tumor cells and are efficient metastatic precursors
The leading cause of breast cancer‐associated death is metastasis. In 80% of solid tumors, metastasis via the lymphatic system precedes metastasis via the vascular system. However, the molecular properties of tumor cells as they exit the primary tumor into the afferent lymphatics en route to the sen...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-06-01
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| Series: | Molecular Oncology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/1878-0261.12494 |
| _version_ | 1819016461954318336 |
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| author | Sulma I. Mohammed Odalys Torres‐Luquis Elwood Walls Frank Lloyd |
| author_facet | Sulma I. Mohammed Odalys Torres‐Luquis Elwood Walls Frank Lloyd |
| author_sort | Sulma I. Mohammed |
| collection | DOAJ |
| description | The leading cause of breast cancer‐associated death is metastasis. In 80% of solid tumors, metastasis via the lymphatic system precedes metastasis via the vascular system. However, the molecular properties of tumor cells as they exit the primary tumor into the afferent lymphatics en route to the sentinel lymph nodes (SLNs) are not yet known. Here, we developed an innovative technique that enables the collection of lymph and lymph‐circulating tumor cells (LCTCs) en route to the SLN in an immunocompetent animal model of breast cancer metastasis. We found that the gene and protein expression profiles of LCTCs and blood‐circulating tumor cells (BCTCs) as they exit the primary tumor are similar, but distinct from those of primary tumors and lymph node metastases (LNMs). LCTCs, but not BCTCs, exist in clusters, display a hybrid epithelial/mesenchymal phenotype and cancer stem cell‐like properties, and are efficient metastatic precursors. These results demonstrate that tumor cells that metastasize through the lymphatic system are different from those spread by blood circulation. Understanding the relative contribution of these cells to overall peripheral blood‐circulating tumor cells is important for cancer therapy. Whether these two types of cell occur in cancer patients remains to be determined. |
| first_indexed | 2024-12-21T02:48:00Z |
| format | Article |
| id | doaj.art-503788f71a1a48a5af87328ba9c52c2c |
| institution | Directory Open Access Journal |
| issn | 1574-7891 1878-0261 |
| language | English |
| last_indexed | 2024-12-21T02:48:00Z |
| publishDate | 2019-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Molecular Oncology |
| spelling | doaj.art-503788f71a1a48a5af87328ba9c52c2c2022-12-21T19:18:31ZengWileyMolecular Oncology1574-78911878-02612019-06-011361400141810.1002/1878-0261.12494Lymph‐circulating tumor cells show distinct properties to blood‐circulating tumor cells and are efficient metastatic precursorsSulma I. Mohammed0Odalys Torres‐Luquis1Elwood Walls2Frank Lloyd3Department of Comparative Pathobiology and Purdue University Center for Cancer Research Purdue University West Lafayette IN USADepartment of Comparative Pathobiology and Purdue University Center for Cancer Research Purdue University West Lafayette IN USADepartment of Basic Medical Sciences Purdue University West Lafayette IN USADepartment of Biomedical Engineering Purdue University West Lafayette IN USAThe leading cause of breast cancer‐associated death is metastasis. In 80% of solid tumors, metastasis via the lymphatic system precedes metastasis via the vascular system. However, the molecular properties of tumor cells as they exit the primary tumor into the afferent lymphatics en route to the sentinel lymph nodes (SLNs) are not yet known. Here, we developed an innovative technique that enables the collection of lymph and lymph‐circulating tumor cells (LCTCs) en route to the SLN in an immunocompetent animal model of breast cancer metastasis. We found that the gene and protein expression profiles of LCTCs and blood‐circulating tumor cells (BCTCs) as they exit the primary tumor are similar, but distinct from those of primary tumors and lymph node metastases (LNMs). LCTCs, but not BCTCs, exist in clusters, display a hybrid epithelial/mesenchymal phenotype and cancer stem cell‐like properties, and are efficient metastatic precursors. These results demonstrate that tumor cells that metastasize through the lymphatic system are different from those spread by blood circulation. Understanding the relative contribution of these cells to overall peripheral blood‐circulating tumor cells is important for cancer therapy. Whether these two types of cell occur in cancer patients remains to be determined.https://doi.org/10.1002/1878-0261.12494breastcancercirculating tumor cellsepithelial‐to‐mesenchymal transitionlymphatic vesselsmetastasis |
| spellingShingle | Sulma I. Mohammed Odalys Torres‐Luquis Elwood Walls Frank Lloyd Lymph‐circulating tumor cells show distinct properties to blood‐circulating tumor cells and are efficient metastatic precursors Molecular Oncology breast cancer circulating tumor cells epithelial‐to‐mesenchymal transition lymphatic vessels metastasis |
| title | Lymph‐circulating tumor cells show distinct properties to blood‐circulating tumor cells and are efficient metastatic precursors |
| title_full | Lymph‐circulating tumor cells show distinct properties to blood‐circulating tumor cells and are efficient metastatic precursors |
| title_fullStr | Lymph‐circulating tumor cells show distinct properties to blood‐circulating tumor cells and are efficient metastatic precursors |
| title_full_unstemmed | Lymph‐circulating tumor cells show distinct properties to blood‐circulating tumor cells and are efficient metastatic precursors |
| title_short | Lymph‐circulating tumor cells show distinct properties to blood‐circulating tumor cells and are efficient metastatic precursors |
| title_sort | lymph circulating tumor cells show distinct properties to blood circulating tumor cells and are efficient metastatic precursors |
| topic | breast cancer circulating tumor cells epithelial‐to‐mesenchymal transition lymphatic vessels metastasis |
| url | https://doi.org/10.1002/1878-0261.12494 |
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