Genome-Wide Identification and Expression Analysis of the <i>CmHAK</i> Gene Family in Melon (<i>Cucumis melo</i> L.)
(1) Background: As the largest family of potassium transporters in plants, KT/HAK/KUP plays an important function in plant growth, development, and stress, especially for potassium-loving plants such as melon. (2) Methods: The members of the KT/HAK/KUP gene family in the melon genome were identified...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/10/1138 |
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| author | Lina Fu Huizhi Wang Xifang Leng Xinsheng Zhang Baoying Xiao Hui Liu Dongxu Xue Yangyang Wang Chunyan Wu Wei Wang |
| author_facet | Lina Fu Huizhi Wang Xifang Leng Xinsheng Zhang Baoying Xiao Hui Liu Dongxu Xue Yangyang Wang Chunyan Wu Wei Wang |
| author_sort | Lina Fu |
| collection | DOAJ |
| description | (1) Background: As the largest family of potassium transporters in plants, KT/HAK/KUP plays an important function in plant growth, development, and stress, especially for potassium-loving plants such as melon. (2) Methods: The members of the KT/HAK/KUP gene family in the melon genome were identified by bioinformatics technology. The gene structure, chromosome location, phylogeny, and expression analysis were comprehensively and systematically analyzed. (3) Results: The results showed that there are 14 members of the KT/HAK/KUP gene family in melon, which are distributed on seven chromosomes. Each member contains 3–11 introns and 4–12 exons, and could be divided into three distinct branches in phylogeny. The number of amino acid residues encoded by each member varies between 610 and 878. In terms of expression, after 12 h of chilling stress, most of the <i>CmHAK</i> genes were expressed in two melon varieties with different chilling resistances. The expression levels of <i>CmHAK6</i> and <i>CmHAK8</i> were downregulated in chilling-resistant varieties, but there was no significant change in chilling-sensitive varieties, indicating that <i>CmHAK6</i> and <i>CmHAK8</i> genes may play a negative regulatory role in chilling-resistant varieties. (4) Conclusions: The study provides a theoretical basis for in-depth analysis of the functions of KT/HAK/KUP gene family and cultivation of high-potassium stress-resistant melon varieties. |
| first_indexed | 2024-03-10T21:12:39Z |
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| id | doaj.art-5038fec7d19a4498af5f145ac8735dbd |
| institution | Directory Open Access Journal |
| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-10T21:12:39Z |
| publishDate | 2023-10-01 |
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| record_format | Article |
| series | Horticulturae |
| spelling | doaj.art-5038fec7d19a4498af5f145ac8735dbd2023-11-19T16:39:53ZengMDPI AGHorticulturae2311-75242023-10-01910113810.3390/horticulturae9101138Genome-Wide Identification and Expression Analysis of the <i>CmHAK</i> Gene Family in Melon (<i>Cucumis melo</i> L.)Lina Fu0Huizhi Wang1Xifang Leng2Xinsheng Zhang3Baoying Xiao4Hui Liu5Dongxu Xue6Yangyang Wang7Chunyan Wu8Wei Wang9College of Horticulture, Jilin Agricultural University, Changchun 130118, ChinaJilin Provincial Horticulture and Specialty Administration Center, Changchun 130021, ChinaJilin Province Product Quality Supervision and Inspection Institute, Changchun 130022, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun 130118, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun 130118, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun 130118, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun 130118, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun 130118, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun 130118, ChinaCollege of Horticulture, Jilin Agricultural University, Changchun 130118, China(1) Background: As the largest family of potassium transporters in plants, KT/HAK/KUP plays an important function in plant growth, development, and stress, especially for potassium-loving plants such as melon. (2) Methods: The members of the KT/HAK/KUP gene family in the melon genome were identified by bioinformatics technology. The gene structure, chromosome location, phylogeny, and expression analysis were comprehensively and systematically analyzed. (3) Results: The results showed that there are 14 members of the KT/HAK/KUP gene family in melon, which are distributed on seven chromosomes. Each member contains 3–11 introns and 4–12 exons, and could be divided into three distinct branches in phylogeny. The number of amino acid residues encoded by each member varies between 610 and 878. In terms of expression, after 12 h of chilling stress, most of the <i>CmHAK</i> genes were expressed in two melon varieties with different chilling resistances. The expression levels of <i>CmHAK6</i> and <i>CmHAK8</i> were downregulated in chilling-resistant varieties, but there was no significant change in chilling-sensitive varieties, indicating that <i>CmHAK6</i> and <i>CmHAK8</i> genes may play a negative regulatory role in chilling-resistant varieties. (4) Conclusions: The study provides a theoretical basis for in-depth analysis of the functions of KT/HAK/KUP gene family and cultivation of high-potassium stress-resistant melon varieties.https://www.mdpi.com/2311-7524/9/10/1138melonKT/HAK/KUPCmHAKbioinformaticschilling stress |
| spellingShingle | Lina Fu Huizhi Wang Xifang Leng Xinsheng Zhang Baoying Xiao Hui Liu Dongxu Xue Yangyang Wang Chunyan Wu Wei Wang Genome-Wide Identification and Expression Analysis of the <i>CmHAK</i> Gene Family in Melon (<i>Cucumis melo</i> L.) Horticulturae melon KT/HAK/KUP CmHAK bioinformatics chilling stress |
| title | Genome-Wide Identification and Expression Analysis of the <i>CmHAK</i> Gene Family in Melon (<i>Cucumis melo</i> L.) |
| title_full | Genome-Wide Identification and Expression Analysis of the <i>CmHAK</i> Gene Family in Melon (<i>Cucumis melo</i> L.) |
| title_fullStr | Genome-Wide Identification and Expression Analysis of the <i>CmHAK</i> Gene Family in Melon (<i>Cucumis melo</i> L.) |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of the <i>CmHAK</i> Gene Family in Melon (<i>Cucumis melo</i> L.) |
| title_short | Genome-Wide Identification and Expression Analysis of the <i>CmHAK</i> Gene Family in Melon (<i>Cucumis melo</i> L.) |
| title_sort | genome wide identification and expression analysis of the i cmhak i gene family in melon i cucumis melo i l |
| topic | melon KT/HAK/KUP CmHAK bioinformatics chilling stress |
| url | https://www.mdpi.com/2311-7524/9/10/1138 |
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