Diagnostic efficiency on ultrasound shear wave elastography in evaluation of steatosis severity for non-alcoholic fatty liver disease: a rat model
Abstract Background The pathological feature of steatosis affects the elasticity values measured by shear wave elastography (SWE) is still controversial in non-alcoholic fatty liver disease (NAFLD). The aim of this study is to demonstrate the influence of steatosis on liver stiffness measured by SWE...
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40001-023-01042-5 |
| _version_ | 1811165995526520832 |
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| author | Yuhui Wu Qianjiao Liu Yan Wang Fangyan Li Lawrence Wing-Chi Chan Yong Wen Fan Yang Yining Xiang Qinghong Duan Peng Luo Pinggui Lei |
| author_facet | Yuhui Wu Qianjiao Liu Yan Wang Fangyan Li Lawrence Wing-Chi Chan Yong Wen Fan Yang Yining Xiang Qinghong Duan Peng Luo Pinggui Lei |
| author_sort | Yuhui Wu |
| collection | DOAJ |
| description | Abstract Background The pathological feature of steatosis affects the elasticity values measured by shear wave elastography (SWE) is still controversial in non-alcoholic fatty liver disease (NAFLD). The aim of this study is to demonstrate the influence of steatosis on liver stiffness measured by SWE on a rat model with NAFLD and analyze feasibility of SWE for grading steatosis in absence of fibrosis. Methods Sixty-six rats were fed with methionine choline deficient diet or standard diet to produce various stages of steatosis; 48 rats were available for final analysis. Rats underwent abdominal ultrasound SWE examination and pathological assessment. Liver histopathology was analyzed to assess the degree of steatosis, inflammation, ballooning, and fibrosis according to the non-alcoholic fatty liver disease activity score. The diagnostic performance of SWE for differentiating steatosis stages was estimated according to the receiver operating characteristic (ROC) curve. Decision curve analysis (DCA) was conducted to determine clinical usefulness and the areas under DCA (AUDCAs) calculated. Results In multivariate analysis, steatosis was an independent factor affecting the mean elastic modules (B = 1.558, P < 0.001), but not inflammation (B = − 0.031, P = 0.920) and ballooning (B = 0.216, P = 0.458). After adjusting for inflammation and ballooning, the AUROC of the mean elasticity for identifying S ≥ S1 was 0.956 (95%CI: 0.872–0.998) and the AUDCA, 0.621. The AUROC for distinguishing S ≥ S2 and S = S3 was 0.987 (95%CI: 0.951–1.000) and 0.920 (95%CI: 0.816–0.986) and the AUDCA was 0.506 and 0.256, respectively. Conclusions Steatosis is associated with liver stiffness and SWE may have the feasibility to be introduced as an assistive technology in grading steatosis for patients with NAFLD in absence of fibrosis. |
| first_indexed | 2024-04-10T15:45:12Z |
| format | Article |
| id | doaj.art-503c370e15294488bf22c0f003b58df0 |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-04-10T15:45:12Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-503c370e15294488bf22c0f003b58df02023-02-12T12:07:32ZengBMCEuropean Journal of Medical Research2047-783X2023-02-0128111010.1186/s40001-023-01042-5Diagnostic efficiency on ultrasound shear wave elastography in evaluation of steatosis severity for non-alcoholic fatty liver disease: a rat modelYuhui Wu0Qianjiao Liu1Yan Wang2Fangyan Li3Lawrence Wing-Chi Chan4Yong Wen5Fan Yang6Yining Xiang7Qinghong Duan8Peng Luo9Pinggui Lei10Department of Radiology, the Affiliated Hospital of Guizhou Medical UniversityDepartment of Radiology, the Affiliated Hospital of Guizhou Medical UniversityDepartment of Radiology, the Affiliated Hospital of Guizhou Medical UniversityDepartment of Radiology, the Affiliated Hospital of Guizhou Medical UniversityDepartment of Health Technology and Informatics, The Hong Kong Polytechnic UniversityDepartment of Radiology, the Affiliated Hospital of Guizhou Medical UniversitySchool of Biology & Engineering, Guizhou Medical UniversityDepartment of Pathology, The Affiliated Hospital of Guizhou Medical UniversityDepartment of Radiology, the Affiliated Hospital of Guizhou Medical UniversitySchool of Public Health, Guizhou Medical UniversityDepartment of Radiology, the Affiliated Hospital of Guizhou Medical UniversityAbstract Background The pathological feature of steatosis affects the elasticity values measured by shear wave elastography (SWE) is still controversial in non-alcoholic fatty liver disease (NAFLD). The aim of this study is to demonstrate the influence of steatosis on liver stiffness measured by SWE on a rat model with NAFLD and analyze feasibility of SWE for grading steatosis in absence of fibrosis. Methods Sixty-six rats were fed with methionine choline deficient diet or standard diet to produce various stages of steatosis; 48 rats were available for final analysis. Rats underwent abdominal ultrasound SWE examination and pathological assessment. Liver histopathology was analyzed to assess the degree of steatosis, inflammation, ballooning, and fibrosis according to the non-alcoholic fatty liver disease activity score. The diagnostic performance of SWE for differentiating steatosis stages was estimated according to the receiver operating characteristic (ROC) curve. Decision curve analysis (DCA) was conducted to determine clinical usefulness and the areas under DCA (AUDCAs) calculated. Results In multivariate analysis, steatosis was an independent factor affecting the mean elastic modules (B = 1.558, P < 0.001), but not inflammation (B = − 0.031, P = 0.920) and ballooning (B = 0.216, P = 0.458). After adjusting for inflammation and ballooning, the AUROC of the mean elasticity for identifying S ≥ S1 was 0.956 (95%CI: 0.872–0.998) and the AUDCA, 0.621. The AUROC for distinguishing S ≥ S2 and S = S3 was 0.987 (95%CI: 0.951–1.000) and 0.920 (95%CI: 0.816–0.986) and the AUDCA was 0.506 and 0.256, respectively. Conclusions Steatosis is associated with liver stiffness and SWE may have the feasibility to be introduced as an assistive technology in grading steatosis for patients with NAFLD in absence of fibrosis.https://doi.org/10.1186/s40001-023-01042-5Shear wave elastographyNon-alcoholic fatty liver diseaseHepatic steatosisMethionine choline deficiencyFat quantification |
| spellingShingle | Yuhui Wu Qianjiao Liu Yan Wang Fangyan Li Lawrence Wing-Chi Chan Yong Wen Fan Yang Yining Xiang Qinghong Duan Peng Luo Pinggui Lei Diagnostic efficiency on ultrasound shear wave elastography in evaluation of steatosis severity for non-alcoholic fatty liver disease: a rat model European Journal of Medical Research Shear wave elastography Non-alcoholic fatty liver disease Hepatic steatosis Methionine choline deficiency Fat quantification |
| title | Diagnostic efficiency on ultrasound shear wave elastography in evaluation of steatosis severity for non-alcoholic fatty liver disease: a rat model |
| title_full | Diagnostic efficiency on ultrasound shear wave elastography in evaluation of steatosis severity for non-alcoholic fatty liver disease: a rat model |
| title_fullStr | Diagnostic efficiency on ultrasound shear wave elastography in evaluation of steatosis severity for non-alcoholic fatty liver disease: a rat model |
| title_full_unstemmed | Diagnostic efficiency on ultrasound shear wave elastography in evaluation of steatosis severity for non-alcoholic fatty liver disease: a rat model |
| title_short | Diagnostic efficiency on ultrasound shear wave elastography in evaluation of steatosis severity for non-alcoholic fatty liver disease: a rat model |
| title_sort | diagnostic efficiency on ultrasound shear wave elastography in evaluation of steatosis severity for non alcoholic fatty liver disease a rat model |
| topic | Shear wave elastography Non-alcoholic fatty liver disease Hepatic steatosis Methionine choline deficiency Fat quantification |
| url | https://doi.org/10.1186/s40001-023-01042-5 |
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