Allergic Potency of Japanese Cedar Pollen Cry j 1 Is Reduced by a Low Concentration of Hypochlorous Acid Generated by Electolysis
Background: Although Japanese cedar (Cryptomeria japonica) pollinosis has developed into a health problem, few methods eradicate indoor allergens completely. In a recent study, however, the effectiveness of inactivation with sodium hypochlorite (NaOCl) treatment was revealed. Therefore, the present...
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| Format: | Article |
| Language: | English |
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Elsevier
2009-01-01
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| Series: | Allergology International |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1323893015307048 |
| _version_ | 1819054532331569152 |
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| author | Tetsuya Terada Yoko Akimoto Sawako Hyo Daisuke Suzuki Kazuhiko Tanaka Hiroshi Takenaka |
| author_facet | Tetsuya Terada Yoko Akimoto Sawako Hyo Daisuke Suzuki Kazuhiko Tanaka Hiroshi Takenaka |
| author_sort | Tetsuya Terada |
| collection | DOAJ |
| description | Background: Although Japanese cedar (Cryptomeria japonica) pollinosis has developed into a health problem, few methods eradicate indoor allergens completely. In a recent study, however, the effectiveness of inactivation with sodium hypochlorite (NaOCl) treatment was revealed. Therefore, the present study aimed to elucidate the ability of chlorine bleach (NaOCl) to reduce the immunogenicity of the major allergenic protein of Japanese cedar (Cry j 1).
Methods: Sodium dodecyl sulfate polyacrylamide gel electrophoresis, Western blotting, enzyme-linked immunosorbant assay, and skin testing were carried out in 7 individuals.
Results: The allergenic protein was undetectable using sodium dodecyl sulfate polyacrylamide gel electrophoresis and silver staining at a sodium hypochlorite/allergenic protein molar ratio of 457. Western blotting with human sera showed the same dose-dependent efficacy. The immunogenicity of the purified protein and cedar pollen was also demonstrated on enzyme-linked immunosorbant assay to be reduced by sodium hypochlorite treatment in a dose- and time-dependent manner. Moreover, sodium hypochlorite-treatment inhibited the skin test response to the protein in all 7 individuals.
Conclusions: Hypochlorous acid generated by electrolysis is an effective method for significantly reducing the immunogenicity of Cry j 1. |
| first_indexed | 2024-12-21T12:53:07Z |
| format | Article |
| id | doaj.art-503d709993674433a9acebe284920648 |
| institution | Directory Open Access Journal |
| issn | 1323-8930 |
| language | English |
| last_indexed | 2024-12-21T12:53:07Z |
| publishDate | 2009-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Allergology International |
| spelling | doaj.art-503d709993674433a9acebe2849206482022-12-21T19:03:26ZengElsevierAllergology International1323-89302009-01-0158225526010.2332/allergolint.08-OA-0043Allergic Potency of Japanese Cedar Pollen Cry j 1 Is Reduced by a Low Concentration of Hypochlorous Acid Generated by ElectolysisTetsuya Terada0Yoko Akimoto1Sawako Hyo2Daisuke Suzuki3Kazuhiko Tanaka4Hiroshi Takenaka5Department of Otorhinolaryngology, Osaka Medical College, Osaka, JapanDepartment of Otorhinolaryngology, Osaka Medical College, Osaka, JapanDepartment of Otorhinolaryngology, Osaka Medical College, Osaka, JapanR & D H.Q. Human Ecology Research Center, SANYO Electric Co., Gunma, Japan.Clinical Pharmacy and Clinical Pharmacokinetics, Osaka University of Pharmaceutical Sciences, Osaka, JapanDepartment of Otorhinolaryngology, Osaka Medical College, Osaka, JapanBackground: Although Japanese cedar (Cryptomeria japonica) pollinosis has developed into a health problem, few methods eradicate indoor allergens completely. In a recent study, however, the effectiveness of inactivation with sodium hypochlorite (NaOCl) treatment was revealed. Therefore, the present study aimed to elucidate the ability of chlorine bleach (NaOCl) to reduce the immunogenicity of the major allergenic protein of Japanese cedar (Cry j 1). Methods: Sodium dodecyl sulfate polyacrylamide gel electrophoresis, Western blotting, enzyme-linked immunosorbant assay, and skin testing were carried out in 7 individuals. Results: The allergenic protein was undetectable using sodium dodecyl sulfate polyacrylamide gel electrophoresis and silver staining at a sodium hypochlorite/allergenic protein molar ratio of 457. Western blotting with human sera showed the same dose-dependent efficacy. The immunogenicity of the purified protein and cedar pollen was also demonstrated on enzyme-linked immunosorbant assay to be reduced by sodium hypochlorite treatment in a dose- and time-dependent manner. Moreover, sodium hypochlorite-treatment inhibited the skin test response to the protein in all 7 individuals. Conclusions: Hypochlorous acid generated by electrolysis is an effective method for significantly reducing the immunogenicity of Cry j 1.http://www.sciencedirect.com/science/article/pii/S1323893015307048Cry j 1humanhypochlorous acidimmunogenicityJapanese cedar pollinosis |
| spellingShingle | Tetsuya Terada Yoko Akimoto Sawako Hyo Daisuke Suzuki Kazuhiko Tanaka Hiroshi Takenaka Allergic Potency of Japanese Cedar Pollen Cry j 1 Is Reduced by a Low Concentration of Hypochlorous Acid Generated by Electolysis Allergology International Cry j 1 human hypochlorous acid immunogenicity Japanese cedar pollinosis |
| title | Allergic Potency of Japanese Cedar Pollen Cry j 1 Is Reduced by a Low Concentration of Hypochlorous Acid Generated by Electolysis |
| title_full | Allergic Potency of Japanese Cedar Pollen Cry j 1 Is Reduced by a Low Concentration of Hypochlorous Acid Generated by Electolysis |
| title_fullStr | Allergic Potency of Japanese Cedar Pollen Cry j 1 Is Reduced by a Low Concentration of Hypochlorous Acid Generated by Electolysis |
| title_full_unstemmed | Allergic Potency of Japanese Cedar Pollen Cry j 1 Is Reduced by a Low Concentration of Hypochlorous Acid Generated by Electolysis |
| title_short | Allergic Potency of Japanese Cedar Pollen Cry j 1 Is Reduced by a Low Concentration of Hypochlorous Acid Generated by Electolysis |
| title_sort | allergic potency of japanese cedar pollen cry j 1 is reduced by a low concentration of hypochlorous acid generated by electolysis |
| topic | Cry j 1 human hypochlorous acid immunogenicity Japanese cedar pollinosis |
| url | http://www.sciencedirect.com/science/article/pii/S1323893015307048 |
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