Ameliorative Effects of <i>Lactobacillus plantarum</i> HAC01 Lysate on 3T3-L1 Adipocyte Differentiation via AMPK Activation and MAPK Inhibition
<i>Lactobacillus plantarum</i> HAC01 has been shown to effectively treat metabolic diseases. However, the precise pharmacological effects and molecular mechanisms of <i>L. plantarum</i> HAC01 remain unclear. In this study, we investigate the anti-adipogenic effects of <i&g...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/11/5901 |
| Summary: | <i>Lactobacillus plantarum</i> HAC01 has been shown to effectively treat metabolic diseases. However, the precise pharmacological effects and molecular mechanisms of <i>L. plantarum</i> HAC01 remain unclear. In this study, we investigate the anti-adipogenic effects of <i>L. plantarum</i> HAC01 lysate and its associated mechanism of action. To induce lipid accumulation, 3T3-L1 cells were incubated in differentiation media with or without <i>L. plantarum</i> HAC01 lysate. Our results show that <i>L. plantarum</i> HAC01 lysate treatment not only reduced lipid accumulation during the differentiation of 3T3-L1 cells, but also decreased the expression of adipogenic and lipogenic genes involved in lipid metabolism in a dose-dependent manner. Additionally, <i>L. plantarum</i> HAC01 lysate inhibited CCAAT/enhancer-binding protein (C/EBP) beta within 4 h of differentiation induction and inhibited peroxisome proliferator-activated receptor gamma, C/EBP alpha, and sterol regulatory element-binding proteins within 2 d. Moreover, treatment with <i>L. plantarum</i> HAC01 lysate increased the phosphorylation of adenosine monophosphate-activated protein kinase, an important regulator of energy metabolism, and decreased the phosphorylation of mitogen-activated protein kinase. These results indicate that <i>L. plantarum</i> HAC01 lysate may have anti-adipogenic effects and support its potential as a useful agent for the treatment of obesity. |
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| ISSN: | 1661-6596 1422-0067 |