Ameliorative Effects of <i>Lactobacillus plantarum</i> HAC01 Lysate on 3T3-L1 Adipocyte Differentiation via AMPK Activation and MAPK Inhibition
<i>Lactobacillus plantarum</i> HAC01 has been shown to effectively treat metabolic diseases. However, the precise pharmacological effects and molecular mechanisms of <i>L. plantarum</i> HAC01 remain unclear. In this study, we investigate the anti-adipogenic effects of <i&g...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/11/5901 |
| _version_ | 1797493295241232384 |
|---|---|
| author | Jong-Yeon Kim Eun-Jung Park Hae-Jeung Lee |
| author_facet | Jong-Yeon Kim Eun-Jung Park Hae-Jeung Lee |
| author_sort | Jong-Yeon Kim |
| collection | DOAJ |
| description | <i>Lactobacillus plantarum</i> HAC01 has been shown to effectively treat metabolic diseases. However, the precise pharmacological effects and molecular mechanisms of <i>L. plantarum</i> HAC01 remain unclear. In this study, we investigate the anti-adipogenic effects of <i>L. plantarum</i> HAC01 lysate and its associated mechanism of action. To induce lipid accumulation, 3T3-L1 cells were incubated in differentiation media with or without <i>L. plantarum</i> HAC01 lysate. Our results show that <i>L. plantarum</i> HAC01 lysate treatment not only reduced lipid accumulation during the differentiation of 3T3-L1 cells, but also decreased the expression of adipogenic and lipogenic genes involved in lipid metabolism in a dose-dependent manner. Additionally, <i>L. plantarum</i> HAC01 lysate inhibited CCAAT/enhancer-binding protein (C/EBP) beta within 4 h of differentiation induction and inhibited peroxisome proliferator-activated receptor gamma, C/EBP alpha, and sterol regulatory element-binding proteins within 2 d. Moreover, treatment with <i>L. plantarum</i> HAC01 lysate increased the phosphorylation of adenosine monophosphate-activated protein kinase, an important regulator of energy metabolism, and decreased the phosphorylation of mitogen-activated protein kinase. These results indicate that <i>L. plantarum</i> HAC01 lysate may have anti-adipogenic effects and support its potential as a useful agent for the treatment of obesity. |
| first_indexed | 2024-03-10T01:17:58Z |
| format | Article |
| id | doaj.art-503d8bface0645e4a10270455636e7c5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:17:58Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-503d8bface0645e4a10270455636e7c52023-11-23T14:06:06ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-05-012311590110.3390/ijms23115901Ameliorative Effects of <i>Lactobacillus plantarum</i> HAC01 Lysate on 3T3-L1 Adipocyte Differentiation via AMPK Activation and MAPK InhibitionJong-Yeon Kim0Eun-Jung Park1Hae-Jeung Lee2Department of Food Science and Biotechnology, Gachon University, Seongnam 13120, Gyeonggi-do, KoreaDepartment of Food and Nutrition, Gachon University, Seongnam 13120, Gyeonggi-do, KoreaDepartment of Food Science and Biotechnology, Gachon University, Seongnam 13120, Gyeonggi-do, Korea<i>Lactobacillus plantarum</i> HAC01 has been shown to effectively treat metabolic diseases. However, the precise pharmacological effects and molecular mechanisms of <i>L. plantarum</i> HAC01 remain unclear. In this study, we investigate the anti-adipogenic effects of <i>L. plantarum</i> HAC01 lysate and its associated mechanism of action. To induce lipid accumulation, 3T3-L1 cells were incubated in differentiation media with or without <i>L. plantarum</i> HAC01 lysate. Our results show that <i>L. plantarum</i> HAC01 lysate treatment not only reduced lipid accumulation during the differentiation of 3T3-L1 cells, but also decreased the expression of adipogenic and lipogenic genes involved in lipid metabolism in a dose-dependent manner. Additionally, <i>L. plantarum</i> HAC01 lysate inhibited CCAAT/enhancer-binding protein (C/EBP) beta within 4 h of differentiation induction and inhibited peroxisome proliferator-activated receptor gamma, C/EBP alpha, and sterol regulatory element-binding proteins within 2 d. Moreover, treatment with <i>L. plantarum</i> HAC01 lysate increased the phosphorylation of adenosine monophosphate-activated protein kinase, an important regulator of energy metabolism, and decreased the phosphorylation of mitogen-activated protein kinase. These results indicate that <i>L. plantarum</i> HAC01 lysate may have anti-adipogenic effects and support its potential as a useful agent for the treatment of obesity.https://www.mdpi.com/1422-0067/23/11/5901<i>Lactobacillus plantarum</i> HAC01anti-obesitylipid metabolismAMPKMAPK |
| spellingShingle | Jong-Yeon Kim Eun-Jung Park Hae-Jeung Lee Ameliorative Effects of <i>Lactobacillus plantarum</i> HAC01 Lysate on 3T3-L1 Adipocyte Differentiation via AMPK Activation and MAPK Inhibition International Journal of Molecular Sciences <i>Lactobacillus plantarum</i> HAC01 anti-obesity lipid metabolism AMPK MAPK |
| title | Ameliorative Effects of <i>Lactobacillus plantarum</i> HAC01 Lysate on 3T3-L1 Adipocyte Differentiation via AMPK Activation and MAPK Inhibition |
| title_full | Ameliorative Effects of <i>Lactobacillus plantarum</i> HAC01 Lysate on 3T3-L1 Adipocyte Differentiation via AMPK Activation and MAPK Inhibition |
| title_fullStr | Ameliorative Effects of <i>Lactobacillus plantarum</i> HAC01 Lysate on 3T3-L1 Adipocyte Differentiation via AMPK Activation and MAPK Inhibition |
| title_full_unstemmed | Ameliorative Effects of <i>Lactobacillus plantarum</i> HAC01 Lysate on 3T3-L1 Adipocyte Differentiation via AMPK Activation and MAPK Inhibition |
| title_short | Ameliorative Effects of <i>Lactobacillus plantarum</i> HAC01 Lysate on 3T3-L1 Adipocyte Differentiation via AMPK Activation and MAPK Inhibition |
| title_sort | ameliorative effects of i lactobacillus plantarum i hac01 lysate on 3t3 l1 adipocyte differentiation via ampk activation and mapk inhibition |
| topic | <i>Lactobacillus plantarum</i> HAC01 anti-obesity lipid metabolism AMPK MAPK |
| url | https://www.mdpi.com/1422-0067/23/11/5901 |
| work_keys_str_mv | AT jongyeonkim ameliorativeeffectsofilactobacillusplantarumihac01lysateon3t3l1adipocytedifferentiationviaampkactivationandmapkinhibition AT eunjungpark ameliorativeeffectsofilactobacillusplantarumihac01lysateon3t3l1adipocytedifferentiationviaampkactivationandmapkinhibition AT haejeunglee ameliorativeeffectsofilactobacillusplantarumihac01lysateon3t3l1adipocytedifferentiationviaampkactivationandmapkinhibition |