Bromelain regulates TRP channels to induce orofacial nociception relief in adult zebrafish
Bromelain, the main protease enzyme found in the pineapple plant (Ananas comosus), has had its antinociceptive effect previously demonstrated. This investigation aimed to appraise the role of TRP (Transient Receptor Potential) channels in the nociception-relieving effects of bromelain in the orofaci...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-03-01
|
| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580823001796 |
| _version_ | 1797317118152146944 |
|---|---|
| author | Saulo Rodrigo Lucas Ribeiro Sacha Aubrey Alves Rodrigues Santos Ana Lívia Oliveira de Sousa Rodrigues Gabriella Mesquita de Sena Antônio Eufrásio Vieira-Neto Adriana Rolim Campos |
| author_facet | Saulo Rodrigo Lucas Ribeiro Sacha Aubrey Alves Rodrigues Santos Ana Lívia Oliveira de Sousa Rodrigues Gabriella Mesquita de Sena Antônio Eufrásio Vieira-Neto Adriana Rolim Campos |
| author_sort | Saulo Rodrigo Lucas Ribeiro |
| collection | DOAJ |
| description | Bromelain, the main protease enzyme found in the pineapple plant (Ananas comosus), has had its antinociceptive effect previously demonstrated. This investigation aimed to appraise the role of TRP (Transient Receptor Potential) channels in the nociception-relieving effects of bromelain in the orofacial region of adult zebrafish. The animals were pretreated with bromelain (3.0, 10.0 or 30.0 mg/mL; gavage) and submitted to open field and acute orofacial (capsaicin - TRPV1 agonist, cinnamaldehyde - TRPA1 agonist or menthol - TRPM8 agonist) nociception tests. The investigation also explored the contribution of central afferent C-fibers. Naive groups were included for comparison. Bromelain did not independently affect the zebrafish movement patterns. However, bromelain decreased the nociceptive responses elicited by all three TRP channel activators. Capsazepine (TRPV1 inhibitor) and AMTB (TRPM8 inhibitor), but not HC-030031 (TRPA1 inhibitor), prevented the antinociceptive effect of bromelain. Moreover, capsaicin-induced desensitization effectively nullified the antinociceptive effect of bromelain. Collectively, these findings corroborate the therapeutic relevance of bromelain as a suppressor of orofacial nociception, which seems to be intricately connected to the modulation of TRP channels. |
| first_indexed | 2024-03-08T03:30:14Z |
| format | Article |
| id | doaj.art-503ee9e5d9a3486cbd225bf3b3068803 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-03-08T03:30:14Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-503ee9e5d9a3486cbd225bf3b30688032024-02-11T05:11:12ZengElsevierBiochemistry and Biophysics Reports2405-58082024-03-0137101598Bromelain regulates TRP channels to induce orofacial nociception relief in adult zebrafishSaulo Rodrigo Lucas Ribeiro0Sacha Aubrey Alves Rodrigues Santos1Ana Lívia Oliveira de Sousa Rodrigues2Gabriella Mesquita de Sena3Antônio Eufrásio Vieira-Neto4Adriana Rolim Campos5Graduation Program in Medical Sciences, Universidade de Fortaleza, Fortaleza, BrazilExperimental Biology Center, Universidade de Fortaleza, Fortaleza, BrazilExperimental Biology Center, Universidade de Fortaleza, Fortaleza, BrazilExperimental Biology Center, Universidade de Fortaleza, Fortaleza, BrazilExperimental Biology Center, Universidade de Fortaleza, Fortaleza, BrazilGraduation Program in Medical Sciences, Universidade de Fortaleza, Fortaleza, Brazil; Experimental Biology Center, Universidade de Fortaleza, Fortaleza, Brazil; Corresponding author. Universidade de Fortaleza Núcleo de Biologia Experimental (NUBEX), Av. Washington Soares, 1321, CEP 60811-905, Edson Queiroz, Fortaleza, Ceará, Brazil.Bromelain, the main protease enzyme found in the pineapple plant (Ananas comosus), has had its antinociceptive effect previously demonstrated. This investigation aimed to appraise the role of TRP (Transient Receptor Potential) channels in the nociception-relieving effects of bromelain in the orofacial region of adult zebrafish. The animals were pretreated with bromelain (3.0, 10.0 or 30.0 mg/mL; gavage) and submitted to open field and acute orofacial (capsaicin - TRPV1 agonist, cinnamaldehyde - TRPA1 agonist or menthol - TRPM8 agonist) nociception tests. The investigation also explored the contribution of central afferent C-fibers. Naive groups were included for comparison. Bromelain did not independently affect the zebrafish movement patterns. However, bromelain decreased the nociceptive responses elicited by all three TRP channel activators. Capsazepine (TRPV1 inhibitor) and AMTB (TRPM8 inhibitor), but not HC-030031 (TRPA1 inhibitor), prevented the antinociceptive effect of bromelain. Moreover, capsaicin-induced desensitization effectively nullified the antinociceptive effect of bromelain. Collectively, these findings corroborate the therapeutic relevance of bromelain as a suppressor of orofacial nociception, which seems to be intricately connected to the modulation of TRP channels.http://www.sciencedirect.com/science/article/pii/S2405580823001796BromelainAntinociceptionZebrafishTRPs |
| spellingShingle | Saulo Rodrigo Lucas Ribeiro Sacha Aubrey Alves Rodrigues Santos Ana Lívia Oliveira de Sousa Rodrigues Gabriella Mesquita de Sena Antônio Eufrásio Vieira-Neto Adriana Rolim Campos Bromelain regulates TRP channels to induce orofacial nociception relief in adult zebrafish Biochemistry and Biophysics Reports Bromelain Antinociception Zebrafish TRPs |
| title | Bromelain regulates TRP channels to induce orofacial nociception relief in adult zebrafish |
| title_full | Bromelain regulates TRP channels to induce orofacial nociception relief in adult zebrafish |
| title_fullStr | Bromelain regulates TRP channels to induce orofacial nociception relief in adult zebrafish |
| title_full_unstemmed | Bromelain regulates TRP channels to induce orofacial nociception relief in adult zebrafish |
| title_short | Bromelain regulates TRP channels to induce orofacial nociception relief in adult zebrafish |
| title_sort | bromelain regulates trp channels to induce orofacial nociception relief in adult zebrafish |
| topic | Bromelain Antinociception Zebrafish TRPs |
| url | http://www.sciencedirect.com/science/article/pii/S2405580823001796 |
| work_keys_str_mv | AT saulorodrigolucasribeiro bromelainregulatestrpchannelstoinduceorofacialnociceptionreliefinadultzebrafish AT sachaaubreyalvesrodriguessantos bromelainregulatestrpchannelstoinduceorofacialnociceptionreliefinadultzebrafish AT analiviaoliveiradesousarodrigues bromelainregulatestrpchannelstoinduceorofacialnociceptionreliefinadultzebrafish AT gabriellamesquitadesena bromelainregulatestrpchannelstoinduceorofacialnociceptionreliefinadultzebrafish AT antonioeufrasiovieiraneto bromelainregulatestrpchannelstoinduceorofacialnociceptionreliefinadultzebrafish AT adrianarolimcampos bromelainregulatestrpchannelstoinduceorofacialnociceptionreliefinadultzebrafish |