Regulation of enzymatic reactions by chemical composition of peptide biomolecular condensates
Abstract Biomolecular condensates are condensed intracellular phases that are formed by liquid-liquid phase separation (LLPS) of proteins, either in the absence or presence of nucleic acids. These condensed phases regulate various biochemical reactions by recruitment of enzymes and substrates. Devel...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2024-04-01
|
| Series: | Communications Chemistry |
| Online Access: | https://doi.org/10.1038/s42004-024-01174-7 |
| _version_ | 1797199604048986112 |
|---|---|
| author | Rif Harris Shirel Veretnik Simran Dewan Avigail Baruch Leshem Ayala Lampel |
| author_facet | Rif Harris Shirel Veretnik Simran Dewan Avigail Baruch Leshem Ayala Lampel |
| author_sort | Rif Harris |
| collection | DOAJ |
| description | Abstract Biomolecular condensates are condensed intracellular phases that are formed by liquid-liquid phase separation (LLPS) of proteins, either in the absence or presence of nucleic acids. These condensed phases regulate various biochemical reactions by recruitment of enzymes and substrates. Developments in the field of LLPS facilitated new insights on the regulation of compartmentalized enzymatic reactions. Yet, the influence of condensate chemical composition on enzymatic reactions is still poorly understood. Here, by using peptides as minimalistic condensate building blocks and β-galactosidase as a simple enzymatic model we show that the reaction is restricted in homotypic peptide condensates, while product formation is enhanced in peptide-RNA condensates. Our findings also show that condensate composition affects the recruitment of substrate, the spatial distribution, and the kinetics of the reaction. Thus, these findings can be further employed for the development of microreactors for biotechnological applications. |
| first_indexed | 2024-04-24T07:18:23Z |
| format | Article |
| id | doaj.art-503f54db13814324a8a646f1f94f2980 |
| institution | Directory Open Access Journal |
| issn | 2399-3669 |
| language | English |
| last_indexed | 2024-04-24T07:18:23Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Chemistry |
| spelling | doaj.art-503f54db13814324a8a646f1f94f29802024-04-21T11:11:41ZengNature PortfolioCommunications Chemistry2399-36692024-04-01711910.1038/s42004-024-01174-7Regulation of enzymatic reactions by chemical composition of peptide biomolecular condensatesRif Harris0Shirel Veretnik1Simran Dewan2Avigail Baruch Leshem3Ayala Lampel4Shmunis School of Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv UniversityShmunis School of Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv UniversityShmunis School of Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv UniversityShmunis School of Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv UniversityShmunis School of Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Tel Aviv UniversityAbstract Biomolecular condensates are condensed intracellular phases that are formed by liquid-liquid phase separation (LLPS) of proteins, either in the absence or presence of nucleic acids. These condensed phases regulate various biochemical reactions by recruitment of enzymes and substrates. Developments in the field of LLPS facilitated new insights on the regulation of compartmentalized enzymatic reactions. Yet, the influence of condensate chemical composition on enzymatic reactions is still poorly understood. Here, by using peptides as minimalistic condensate building blocks and β-galactosidase as a simple enzymatic model we show that the reaction is restricted in homotypic peptide condensates, while product formation is enhanced in peptide-RNA condensates. Our findings also show that condensate composition affects the recruitment of substrate, the spatial distribution, and the kinetics of the reaction. Thus, these findings can be further employed for the development of microreactors for biotechnological applications.https://doi.org/10.1038/s42004-024-01174-7 |
| spellingShingle | Rif Harris Shirel Veretnik Simran Dewan Avigail Baruch Leshem Ayala Lampel Regulation of enzymatic reactions by chemical composition of peptide biomolecular condensates Communications Chemistry |
| title | Regulation of enzymatic reactions by chemical composition of peptide biomolecular condensates |
| title_full | Regulation of enzymatic reactions by chemical composition of peptide biomolecular condensates |
| title_fullStr | Regulation of enzymatic reactions by chemical composition of peptide biomolecular condensates |
| title_full_unstemmed | Regulation of enzymatic reactions by chemical composition of peptide biomolecular condensates |
| title_short | Regulation of enzymatic reactions by chemical composition of peptide biomolecular condensates |
| title_sort | regulation of enzymatic reactions by chemical composition of peptide biomolecular condensates |
| url | https://doi.org/10.1038/s42004-024-01174-7 |
| work_keys_str_mv | AT rifharris regulationofenzymaticreactionsbychemicalcompositionofpeptidebiomolecularcondensates AT shirelveretnik regulationofenzymaticreactionsbychemicalcompositionofpeptidebiomolecularcondensates AT simrandewan regulationofenzymaticreactionsbychemicalcompositionofpeptidebiomolecularcondensates AT avigailbaruchleshem regulationofenzymaticreactionsbychemicalcompositionofpeptidebiomolecularcondensates AT ayalalampel regulationofenzymaticreactionsbychemicalcompositionofpeptidebiomolecularcondensates |