The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, <i>Pollicipes pollicipes</i>
Adhesive secretion has a fundamental role in barnacles’ survival, keeping them in an adequate position on the substrate under a variety of hydrologic regimes. It arouses special interest for industrial applications, such as antifouling strategies, underwater industrial and surgical glues, and dental...
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MDPI AG
2020-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/7/2524 |
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| author | Dany Domínguez-Pérez Daniela Almeida Josef Wissing André M. Machado Lothar Jänsch Luís Filipe Castro Agostinho Antunes Vitor Vasconcelos Alexandre Campos Isabel Cunha |
| author_facet | Dany Domínguez-Pérez Daniela Almeida Josef Wissing André M. Machado Lothar Jänsch Luís Filipe Castro Agostinho Antunes Vitor Vasconcelos Alexandre Campos Isabel Cunha |
| author_sort | Dany Domínguez-Pérez |
| collection | DOAJ |
| description | Adhesive secretion has a fundamental role in barnacles’ survival, keeping them in an adequate position on the substrate under a variety of hydrologic regimes. It arouses special interest for industrial applications, such as antifouling strategies, underwater industrial and surgical glues, and dental composites. This study was focused on the goose barnacle <i>Pollicipes pollicipes</i> adhesion system, a species that lives in the Eastern Atlantic strongly exposed intertidal rocky shores and cliffs. The protein composition of <i>P. pollicipes</i> cement multicomplex and cement gland was quantitatively studied using a label-free LC-MS high-throughput proteomic analysis, searched against a custom transcriptome-derived database. Overall, 11,755 peptide sequences were identified in the gland while 2880 peptide sequences were detected in the cement, clustered in 1616 and 1568 protein groups, respectively. The gland proteome was dominated by proteins of the muscle, cytoskeleton, and some uncharacterized proteins, while the cement was, for the first time, reported to be composed by nearly 50% of proteins that are not canonical cement proteins, mainly unannotated proteins, chemical cues, and protease inhibitors, among others. Bulk adhesive proteins accounted for one-third of the cement proteome, with CP52k being the most abundant. Some unannotated proteins highly expressed in the proteomes, as well as at the transcriptomic level, showed similar physicochemical properties to the known surface-coupling barnacle adhesive proteins while the function of the others remains to be discovered. New quantitative and qualitative clues are provided to understand the diversity and function of proteins in the cement of stalked barnacles, contributing to the whole adhesion model in Cirripedia. |
| first_indexed | 2024-03-10T20:39:47Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T20:39:47Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-50418dc148b040d6947bca98e8f233fb2023-11-19T20:46:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01217252410.3390/ijms21072524The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, <i>Pollicipes pollicipes</i>Dany Domínguez-Pérez0Daniela Almeida1Josef Wissing2André M. Machado3Lothar Jänsch4Luís Filipe Castro5Agostinho Antunes6Vitor Vasconcelos7Alexandre Campos8Isabel Cunha9CIIMAR–Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalCIIMAR–Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalCellular Proteomics Research, Helmholtz Centre for Infection Research, Inhoffenstraße. 7, 38124 Braunschweig, GermanyCIIMAR–Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalCellular Proteomics Research, Helmholtz Centre for Infection Research, Inhoffenstraße. 7, 38124 Braunschweig, GermanyCIIMAR–Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalCIIMAR–Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalCIIMAR–Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalCIIMAR–Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalCIIMAR–Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua General Norton de Matos s/n, Terminal de Cruzeiros do Porto de Leixões, 4450-208 Matosinhos, PortugalAdhesive secretion has a fundamental role in barnacles’ survival, keeping them in an adequate position on the substrate under a variety of hydrologic regimes. It arouses special interest for industrial applications, such as antifouling strategies, underwater industrial and surgical glues, and dental composites. This study was focused on the goose barnacle <i>Pollicipes pollicipes</i> adhesion system, a species that lives in the Eastern Atlantic strongly exposed intertidal rocky shores and cliffs. The protein composition of <i>P. pollicipes</i> cement multicomplex and cement gland was quantitatively studied using a label-free LC-MS high-throughput proteomic analysis, searched against a custom transcriptome-derived database. Overall, 11,755 peptide sequences were identified in the gland while 2880 peptide sequences were detected in the cement, clustered in 1616 and 1568 protein groups, respectively. The gland proteome was dominated by proteins of the muscle, cytoskeleton, and some uncharacterized proteins, while the cement was, for the first time, reported to be composed by nearly 50% of proteins that are not canonical cement proteins, mainly unannotated proteins, chemical cues, and protease inhibitors, among others. Bulk adhesive proteins accounted for one-third of the cement proteome, with CP52k being the most abundant. Some unannotated proteins highly expressed in the proteomes, as well as at the transcriptomic level, showed similar physicochemical properties to the known surface-coupling barnacle adhesive proteins while the function of the others remains to be discovered. New quantitative and qualitative clues are provided to understand the diversity and function of proteins in the cement of stalked barnacles, contributing to the whole adhesion model in Cirripedia.https://www.mdpi.com/1422-0067/21/7/2524underwater adhesioncement proteinQ-ExactiveproteogenomicMaxQuantiBAQ |
| spellingShingle | Dany Domínguez-Pérez Daniela Almeida Josef Wissing André M. Machado Lothar Jänsch Luís Filipe Castro Agostinho Antunes Vitor Vasconcelos Alexandre Campos Isabel Cunha The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, <i>Pollicipes pollicipes</i> International Journal of Molecular Sciences underwater adhesion cement protein Q-Exactive proteogenomic MaxQuant iBAQ |
| title | The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, <i>Pollicipes pollicipes</i> |
| title_full | The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, <i>Pollicipes pollicipes</i> |
| title_fullStr | The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, <i>Pollicipes pollicipes</i> |
| title_full_unstemmed | The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, <i>Pollicipes pollicipes</i> |
| title_short | The Quantitative Proteome of the Cement and Adhesive Gland of the Pedunculate Barnacle, <i>Pollicipes pollicipes</i> |
| title_sort | quantitative proteome of the cement and adhesive gland of the pedunculate barnacle i pollicipes pollicipes i |
| topic | underwater adhesion cement protein Q-Exactive proteogenomic MaxQuant iBAQ |
| url | https://www.mdpi.com/1422-0067/21/7/2524 |
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