Recent Advances in Biological Applications of Aptamer-Based Fluorescent Biosensors
Aptamers have been spotlighted as promising bio-recognition elements because they can be tailored to specific target molecules, bind to targets with a high affinity and specificity, and are easy to chemically synthesize and introduce functional groups to. In particular, fluorescent aptasensors are w...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/21/7327 |
| _version_ | 1797631557759926272 |
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| author | Minhyuk Lee Seonhye Shin Sungjee Kim Nokyoung Park |
| author_facet | Minhyuk Lee Seonhye Shin Sungjee Kim Nokyoung Park |
| author_sort | Minhyuk Lee |
| collection | DOAJ |
| description | Aptamers have been spotlighted as promising bio-recognition elements because they can be tailored to specific target molecules, bind to targets with a high affinity and specificity, and are easy to chemically synthesize and introduce functional groups to. In particular, fluorescent aptasensors are widely used in biological applications to diagnose diseases as well as prevent diseases by detecting cancer cells, viruses, and various biomarkers including nucleic acids and proteins as well as biotoxins and bacteria from food because they have the advantages of a high sensitivity, selectivity, rapidity, a simple detection process, and a low price. We introduce screening methods for isolating aptamers with q high specificity and summarize the sequences and affinities of the aptamers in a table. This review focuses on aptamer-based fluorescence detection sensors for biological applications, from fluorescent probes to mechanisms of action and signal amplification strategies. |
| first_indexed | 2024-03-11T11:25:15Z |
| format | Article |
| id | doaj.art-50430871a4e54f4cb4a7ee6712a7acff |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T11:25:15Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-50430871a4e54f4cb4a7ee6712a7acff2023-11-10T15:08:37ZengMDPI AGMolecules1420-30492023-10-012821732710.3390/molecules28217327Recent Advances in Biological Applications of Aptamer-Based Fluorescent BiosensorsMinhyuk Lee0Seonhye Shin1Sungjee Kim2Nokyoung Park3Department of Chemistry, Pohang University of Science and Technology, Pohang 37673, Republic of KoreaDepartment of Chemistry, The Natural Science Research Institute, Myongji University, 116 Myongji-ro, Yongin-si 17058, Republic of KoreaDepartment of Chemistry, Pohang University of Science and Technology, Pohang 37673, Republic of KoreaDepartment of Chemistry, The Natural Science Research Institute, Myongji University, 116 Myongji-ro, Yongin-si 17058, Republic of KoreaAptamers have been spotlighted as promising bio-recognition elements because they can be tailored to specific target molecules, bind to targets with a high affinity and specificity, and are easy to chemically synthesize and introduce functional groups to. In particular, fluorescent aptasensors are widely used in biological applications to diagnose diseases as well as prevent diseases by detecting cancer cells, viruses, and various biomarkers including nucleic acids and proteins as well as biotoxins and bacteria from food because they have the advantages of a high sensitivity, selectivity, rapidity, a simple detection process, and a low price. We introduce screening methods for isolating aptamers with q high specificity and summarize the sequences and affinities of the aptamers in a table. This review focuses on aptamer-based fluorescence detection sensors for biological applications, from fluorescent probes to mechanisms of action and signal amplification strategies.https://www.mdpi.com/1420-3049/28/21/7327aptameraptamer-based sensorsbiosensorfluorescencesignal amplification strategies |
| spellingShingle | Minhyuk Lee Seonhye Shin Sungjee Kim Nokyoung Park Recent Advances in Biological Applications of Aptamer-Based Fluorescent Biosensors Molecules aptamer aptamer-based sensors biosensor fluorescence signal amplification strategies |
| title | Recent Advances in Biological Applications of Aptamer-Based Fluorescent Biosensors |
| title_full | Recent Advances in Biological Applications of Aptamer-Based Fluorescent Biosensors |
| title_fullStr | Recent Advances in Biological Applications of Aptamer-Based Fluorescent Biosensors |
| title_full_unstemmed | Recent Advances in Biological Applications of Aptamer-Based Fluorescent Biosensors |
| title_short | Recent Advances in Biological Applications of Aptamer-Based Fluorescent Biosensors |
| title_sort | recent advances in biological applications of aptamer based fluorescent biosensors |
| topic | aptamer aptamer-based sensors biosensor fluorescence signal amplification strategies |
| url | https://www.mdpi.com/1420-3049/28/21/7327 |
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