Circulating endothelial cells and procoagulant microparticles in patients with glioblastoma: prognostic value.
AIM: Circulating endothelial cells and microparticles are prognostic factors in cancer. However, their prognostic and predictive value in patients with glioblastoma is unclear. The objective of this study was to investigate the potential prognostic value of circulating endothelial cells and micropar...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3726739?pdf=render |
| _version_ | 1819133336239472640 |
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| author | Gaspar Reynés Virtudes Vila Tania Fleitas Edelmiro Reganon Jaime Font de Mora María Jordá Vicenta Martínez-Sales |
| author_facet | Gaspar Reynés Virtudes Vila Tania Fleitas Edelmiro Reganon Jaime Font de Mora María Jordá Vicenta Martínez-Sales |
| author_sort | Gaspar Reynés |
| collection | DOAJ |
| description | AIM: Circulating endothelial cells and microparticles are prognostic factors in cancer. However, their prognostic and predictive value in patients with glioblastoma is unclear. The objective of this study was to investigate the potential prognostic value of circulating endothelial cells and microparticles in patients with newly diagnosed glioblastoma treated with standard radiotherapy and concomitant temozolomide. In addition, we have analyzed the methylation status of the MGMT promoter. METHODS: Peripheral blood samples were obtained before and at the end of the concomitant treatment. Blood samples from healthy volunteers were also obtained as controls. Endothelial cells were measured by an immunomagnetic technique and immunofluorescence microscopy. Microparticles were quantified by flow cytometry. Microparticle-mediated procoagulant activity was measured by endogen thrombin generation and by phospholipid-dependent clotting time. Methylation status of MGMT promoter was determined by multiplex ligation-dependent probe amplification. RESULTS: Pretreatment levels of circulating endothelial cells and microparticles were higher in patients than in controls (p<0.001). After treatment, levels of microparticles and thrombin generation decreased, and phospholipid-dependent clotting time increased significantly. A high pretreatment endothelial cell count, corresponding to the 99(th) percentile in controls, was associated with poor overall survival. MGMT promoter methylation was present in 27% of tumor samples and was associated to a higher overall survival (66 weeks vs 30 weeks, p<0.004). CONCLUSION: Levels of circulating endothelial cells may have prognostic value in patients with glioblastoma. |
| first_indexed | 2024-12-22T09:45:40Z |
| format | Article |
| id | doaj.art-5044acb44b3142fca1c794ff1d3b1f52 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T09:45:40Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5044acb44b3142fca1c794ff1d3b1f522022-12-21T18:30:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0187e6903410.1371/journal.pone.0069034Circulating endothelial cells and procoagulant microparticles in patients with glioblastoma: prognostic value.Gaspar ReynésVirtudes VilaTania FleitasEdelmiro ReganonJaime Font de MoraMaría JordáVicenta Martínez-SalesAIM: Circulating endothelial cells and microparticles are prognostic factors in cancer. However, their prognostic and predictive value in patients with glioblastoma is unclear. The objective of this study was to investigate the potential prognostic value of circulating endothelial cells and microparticles in patients with newly diagnosed glioblastoma treated with standard radiotherapy and concomitant temozolomide. In addition, we have analyzed the methylation status of the MGMT promoter. METHODS: Peripheral blood samples were obtained before and at the end of the concomitant treatment. Blood samples from healthy volunteers were also obtained as controls. Endothelial cells were measured by an immunomagnetic technique and immunofluorescence microscopy. Microparticles were quantified by flow cytometry. Microparticle-mediated procoagulant activity was measured by endogen thrombin generation and by phospholipid-dependent clotting time. Methylation status of MGMT promoter was determined by multiplex ligation-dependent probe amplification. RESULTS: Pretreatment levels of circulating endothelial cells and microparticles were higher in patients than in controls (p<0.001). After treatment, levels of microparticles and thrombin generation decreased, and phospholipid-dependent clotting time increased significantly. A high pretreatment endothelial cell count, corresponding to the 99(th) percentile in controls, was associated with poor overall survival. MGMT promoter methylation was present in 27% of tumor samples and was associated to a higher overall survival (66 weeks vs 30 weeks, p<0.004). CONCLUSION: Levels of circulating endothelial cells may have prognostic value in patients with glioblastoma.http://europepmc.org/articles/PMC3726739?pdf=render |
| spellingShingle | Gaspar Reynés Virtudes Vila Tania Fleitas Edelmiro Reganon Jaime Font de Mora María Jordá Vicenta Martínez-Sales Circulating endothelial cells and procoagulant microparticles in patients with glioblastoma: prognostic value. PLoS ONE |
| title | Circulating endothelial cells and procoagulant microparticles in patients with glioblastoma: prognostic value. |
| title_full | Circulating endothelial cells and procoagulant microparticles in patients with glioblastoma: prognostic value. |
| title_fullStr | Circulating endothelial cells and procoagulant microparticles in patients with glioblastoma: prognostic value. |
| title_full_unstemmed | Circulating endothelial cells and procoagulant microparticles in patients with glioblastoma: prognostic value. |
| title_short | Circulating endothelial cells and procoagulant microparticles in patients with glioblastoma: prognostic value. |
| title_sort | circulating endothelial cells and procoagulant microparticles in patients with glioblastoma prognostic value |
| url | http://europepmc.org/articles/PMC3726739?pdf=render |
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