Improving drought tolerance in soybean by classical breeding leads to physiological adjustments of photosynthesis and stomata functioning
Soybean (Glycine max) is one of the main crops cultivated worldwide and drought is the main abiotic stress affecting its production. However, breeding towards drought tolerance is often set aside due to the difficulties in phenotyping. The aim of this study was to design an effective and simple scre...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Plant Stress |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X23001422 |
| _version_ | 1797422494798315520 |
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| author | Josefina Demicheli Ivana Sabljic Gaston Beguy Edmundo Ploschuk Mariam Sahrawy Antonio J. Serrato Eduardo A. Pagano |
| author_facet | Josefina Demicheli Ivana Sabljic Gaston Beguy Edmundo Ploschuk Mariam Sahrawy Antonio J. Serrato Eduardo A. Pagano |
| author_sort | Josefina Demicheli |
| collection | DOAJ |
| description | Soybean (Glycine max) is one of the main crops cultivated worldwide and drought is the main abiotic stress affecting its production. However, breeding towards drought tolerance is often set aside due to the difficulties in phenotyping. The aim of this study was to design an effective and simple screening technique that allows to discriminate genotypes according to their drought response and to identify physiological traits responsible for a superior tolerance. This rapid screening evaluated morphological (shoot and root length and weight) and developmental traits (days to germination and plant establishment) of 604 genotypes under control and drought conditions. Two genotypes with contrasting responses (Sensitive Genotype and Tolerant Genotype, SG and TG, respectively) were selected according to their performance in the screening, and were subjected to further testing in order to physiologically characterize the drought response of the chosen cultivars. Wilting, normalized transpiration ratio, leaf gas exchange parameters, water use efficiency (WUE) and stomatal characterizations were performed. TG plants exhibited slow wilting and higher WUE when subjected to drought treatments, product of a reduced water loss due to an anticipated stomatal closure which allowed to preserve soil moisture. Besides, under control conditions, the TG genotype presented a higher stomatal aperture index and a bigger density of epidermal cells and stomata than the drought-sensitive genotypes analyzed. In summary, the analysis of easily quantifiable traits, as the growth of the plant aerial organs and root development, together with the plant development timing, allowed us to select two soybean cultivars with contrasting drought responses. In our opinion, this selection method might be efficiently applied for the selection of drought tolerant cultivars in plant species of agronomic interest. |
| first_indexed | 2024-03-09T07:33:07Z |
| format | Article |
| id | doaj.art-5046ff008df045568c5b29511b020d67 |
| institution | Directory Open Access Journal |
| issn | 2667-064X |
| language | English |
| last_indexed | 2024-03-09T07:33:07Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj.art-5046ff008df045568c5b29511b020d672023-12-03T05:43:38ZengElsevierPlant Stress2667-064X2023-12-0110100275Improving drought tolerance in soybean by classical breeding leads to physiological adjustments of photosynthesis and stomata functioningJosefina Demicheli0Ivana Sabljic1Gaston Beguy2Edmundo Ploschuk3Mariam Sahrawy4Antonio J. Serrato5Eduardo A. Pagano6Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Bioquímica. Buenos Aires, ArgentinaUniversidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Bioquímica. Buenos Aires, Argentina; GDM Seeds, Chacabuco, Buenos Aires, ArgentinaUniversidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Bioquímica. Buenos Aires, ArgentinaUniversidad de Buenos Aires. Facultad de Agronomía. Departamento de Producción Vegetal. Cátedra de Cultivos Industriales. Buenos Aires, ArgentinaDepartment of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, 18008 Granada, SpainDepartment of Biochemistry, Cell and Molecular Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, 18008 Granada, Spain; Corresponding author.Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Bioquímica. Buenos Aires, Argentina; CONICET - Universidad de Buenos Aires. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales (INBA. Buenos Aires, ArgentinaSoybean (Glycine max) is one of the main crops cultivated worldwide and drought is the main abiotic stress affecting its production. However, breeding towards drought tolerance is often set aside due to the difficulties in phenotyping. The aim of this study was to design an effective and simple screening technique that allows to discriminate genotypes according to their drought response and to identify physiological traits responsible for a superior tolerance. This rapid screening evaluated morphological (shoot and root length and weight) and developmental traits (days to germination and plant establishment) of 604 genotypes under control and drought conditions. Two genotypes with contrasting responses (Sensitive Genotype and Tolerant Genotype, SG and TG, respectively) were selected according to their performance in the screening, and were subjected to further testing in order to physiologically characterize the drought response of the chosen cultivars. Wilting, normalized transpiration ratio, leaf gas exchange parameters, water use efficiency (WUE) and stomatal characterizations were performed. TG plants exhibited slow wilting and higher WUE when subjected to drought treatments, product of a reduced water loss due to an anticipated stomatal closure which allowed to preserve soil moisture. Besides, under control conditions, the TG genotype presented a higher stomatal aperture index and a bigger density of epidermal cells and stomata than the drought-sensitive genotypes analyzed. In summary, the analysis of easily quantifiable traits, as the growth of the plant aerial organs and root development, together with the plant development timing, allowed us to select two soybean cultivars with contrasting drought responses. In our opinion, this selection method might be efficiently applied for the selection of drought tolerant cultivars in plant species of agronomic interest.http://www.sciencedirect.com/science/article/pii/S2667064X23001422TranspirationWiltingGas exchangeWater use efficiencyGlycine max |
| spellingShingle | Josefina Demicheli Ivana Sabljic Gaston Beguy Edmundo Ploschuk Mariam Sahrawy Antonio J. Serrato Eduardo A. Pagano Improving drought tolerance in soybean by classical breeding leads to physiological adjustments of photosynthesis and stomata functioning Plant Stress Transpiration Wilting Gas exchange Water use efficiency Glycine max |
| title | Improving drought tolerance in soybean by classical breeding leads to physiological adjustments of photosynthesis and stomata functioning |
| title_full | Improving drought tolerance in soybean by classical breeding leads to physiological adjustments of photosynthesis and stomata functioning |
| title_fullStr | Improving drought tolerance in soybean by classical breeding leads to physiological adjustments of photosynthesis and stomata functioning |
| title_full_unstemmed | Improving drought tolerance in soybean by classical breeding leads to physiological adjustments of photosynthesis and stomata functioning |
| title_short | Improving drought tolerance in soybean by classical breeding leads to physiological adjustments of photosynthesis and stomata functioning |
| title_sort | improving drought tolerance in soybean by classical breeding leads to physiological adjustments of photosynthesis and stomata functioning |
| topic | Transpiration Wilting Gas exchange Water use efficiency Glycine max |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X23001422 |
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