Reversible On-Off Photoswitching of DNA Replication Using a Dumbbell Oligodeoxynucleotide
In most organisms, DNA extension is highly regulated; however, most studies have focused on controlling the initiation of replication, and few have been done to control the regulation of DNA extension. In this study, we adopted a new strategy for azODNs to regulate DNA extension, which is based on a...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/24/8992 |
| _version_ | 1797455997261840384 |
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| author | Yu Wang Heming Ji Jian Ma Hang Luo Yujian He Xinjing Tang Li Wu |
| author_facet | Yu Wang Heming Ji Jian Ma Hang Luo Yujian He Xinjing Tang Li Wu |
| author_sort | Yu Wang |
| collection | DOAJ |
| description | In most organisms, DNA extension is highly regulated; however, most studies have focused on controlling the initiation of replication, and few have been done to control the regulation of DNA extension. In this study, we adopted a new strategy for azODNs to regulate DNA extension, which is based on azobenzene oligonucleotide chimeras regulated by substrate binding affinity, and the conformation of the chimera can be regulated by a light source with a light wavelength of 365 nm. The results showed that the primer was extended with Taq DNA polymerase after visible light treatment, and DNA extension could be effectively hindered with UV light treatment. We also verify the reversibility of the photoregulation of primer extension through photoswitching of dumbbell asODNs by alternate irradiation with UV and visible light. Our method has the advantages of fast and simple, green response and reversible operations, providing a new strategy for regulating gene replication. |
| first_indexed | 2024-03-09T16:02:09Z |
| format | Article |
| id | doaj.art-504a2969aa9f48d2bed233518d769d43 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T16:02:09Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-504a2969aa9f48d2bed233518d769d432023-11-24T17:00:59ZengMDPI AGMolecules1420-30492022-12-012724899210.3390/molecules27248992Reversible On-Off Photoswitching of DNA Replication Using a Dumbbell OligodeoxynucleotideYu Wang0Heming Ji1Jian Ma2Hang Luo3Yujian He4Xinjing Tang5Li Wu6School of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, ChinaSchool of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, ChinaSchool of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, ChinaSchool of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, ChinaSchool of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, ChinaSchool of Chemical Sciences, University of Chinese Academy of Sciences, Beijing 100049, ChinaIn most organisms, DNA extension is highly regulated; however, most studies have focused on controlling the initiation of replication, and few have been done to control the regulation of DNA extension. In this study, we adopted a new strategy for azODNs to regulate DNA extension, which is based on azobenzene oligonucleotide chimeras regulated by substrate binding affinity, and the conformation of the chimera can be regulated by a light source with a light wavelength of 365 nm. The results showed that the primer was extended with Taq DNA polymerase after visible light treatment, and DNA extension could be effectively hindered with UV light treatment. We also verify the reversibility of the photoregulation of primer extension through photoswitching of dumbbell asODNs by alternate irradiation with UV and visible light. Our method has the advantages of fast and simple, green response and reversible operations, providing a new strategy for regulating gene replication.https://www.mdpi.com/1420-3049/27/24/8992DNA extensionazODNsUVcontrol |
| spellingShingle | Yu Wang Heming Ji Jian Ma Hang Luo Yujian He Xinjing Tang Li Wu Reversible On-Off Photoswitching of DNA Replication Using a Dumbbell Oligodeoxynucleotide Molecules DNA extension azODNs UV control |
| title | Reversible On-Off Photoswitching of DNA Replication Using a Dumbbell Oligodeoxynucleotide |
| title_full | Reversible On-Off Photoswitching of DNA Replication Using a Dumbbell Oligodeoxynucleotide |
| title_fullStr | Reversible On-Off Photoswitching of DNA Replication Using a Dumbbell Oligodeoxynucleotide |
| title_full_unstemmed | Reversible On-Off Photoswitching of DNA Replication Using a Dumbbell Oligodeoxynucleotide |
| title_short | Reversible On-Off Photoswitching of DNA Replication Using a Dumbbell Oligodeoxynucleotide |
| title_sort | reversible on off photoswitching of dna replication using a dumbbell oligodeoxynucleotide |
| topic | DNA extension azODNs UV control |
| url | https://www.mdpi.com/1420-3049/27/24/8992 |
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