A Simple Ex Vivo Semiquantitative Fluorescent Imaging Utilizing Planar Laser Scanner: Detection of Reactive Oxygen Species Generation in Mouse Brain and Kidney
Objective: Oxidative stress plays an important role in the onset of many neuronal and peripheral disorders. We examined the feasibility of obtaining semiquantitative fluorescent images of reactive oxygen species (ROS) generation in mouse brain and kidney utilizing a planar laser scanner and dihydroe...
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Format: | Article |
Language: | English |
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SAGE Publications
2019-01-01
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Series: | Molecular Imaging |
Online Access: | https://doi.org/10.1177/1536012118820421 |
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author | Rie Hosoi PhD Sota Sato BS Miho Shukuri PhD Yuka Fujii BS Kenichiro Todoroki PhD Yasushi Arano PhD Toshihiro Sakai PhD Osamu Inoue PhD |
author_facet | Rie Hosoi PhD Sota Sato BS Miho Shukuri PhD Yuka Fujii BS Kenichiro Todoroki PhD Yasushi Arano PhD Toshihiro Sakai PhD Osamu Inoue PhD |
author_sort | Rie Hosoi PhD |
collection | DOAJ |
description | Objective: Oxidative stress plays an important role in the onset of many neuronal and peripheral disorders. We examined the feasibility of obtaining semiquantitative fluorescent images of reactive oxygen species (ROS) generation in mouse brain and kidney utilizing a planar laser scanner and dihydroethidium (DHE). Methods: To investigate ROS generation in brain, sodium nitroprusside was injected into the striatum. Dihydroethidium was injected into the tail vein. After DHE injection, tissue slices were analyzed utilizing a planar laser scanner. For kidney study, cis-diamminedichloroplatinum [II] (cisplatin) was intraperitoneally administrated into mice. Results: Clear and semiquantitative fluorescent images of ROS generation in the mouse brain and kidney were obtained. Furthermore, the fluorescence intensity was stable and not affected by fading. Sodium nitroprusside induced approximately 6 times the fluorescence accumulation in the brain. Cisplatin caused renal injury in all mice, and in comparison with control mice, more than 10 times fluorescence accumulation was observed in the renal medulla with tubular necrosis and vacuolization. Conclusions: We successfully obtained ex vivo semiquantitative fluorescent images of ROS generation utilizing a planar laser scanner and DHE. This simple method is useful for ROS detection in several ROS-related animal models and would be applicable to a variety of biochemical processes. |
first_indexed | 2024-03-07T17:09:42Z |
format | Article |
id | doaj.art-504ab024350e40c2aa1f833d21d2211a |
institution | Directory Open Access Journal |
issn | 1536-0121 |
language | English |
last_indexed | 2024-03-07T17:09:42Z |
publishDate | 2019-01-01 |
publisher | SAGE Publications |
record_format | Article |
series | Molecular Imaging |
spelling | doaj.art-504ab024350e40c2aa1f833d21d2211a2024-03-03T01:20:31ZengSAGE PublicationsMolecular Imaging1536-01212019-01-011810.1177/1536012118820421A Simple Ex Vivo Semiquantitative Fluorescent Imaging Utilizing Planar Laser Scanner: Detection of Reactive Oxygen Species Generation in Mouse Brain and KidneyRie Hosoi PhD0Sota Sato BS1Miho Shukuri PhD2Yuka Fujii BS3Kenichiro Todoroki PhD4Yasushi Arano PhD5Toshihiro Sakai PhD6Osamu Inoue PhD7 Division of Health Sciences, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan Division of Health Sciences, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan Laboratory of Physical Chemistry, Showa Pharmaceutical University, Machida, Tokyo, Japan Division of Health Sciences, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan Department of Analytical and Bio-Analytical Chemistry, School of Pharmaceutical Sciences, University of Shizuoka, Suruga, Shizuoka, Japan Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba, Japan Hanwa Intelligent Medical Center, Hanwa Daini Senboku Hospital, Sakai, Osaka, Japan Hanwa Intelligent Medical Center, Hanwa Daini Senboku Hospital, Sakai, Osaka, JapanObjective: Oxidative stress plays an important role in the onset of many neuronal and peripheral disorders. We examined the feasibility of obtaining semiquantitative fluorescent images of reactive oxygen species (ROS) generation in mouse brain and kidney utilizing a planar laser scanner and dihydroethidium (DHE). Methods: To investigate ROS generation in brain, sodium nitroprusside was injected into the striatum. Dihydroethidium was injected into the tail vein. After DHE injection, tissue slices were analyzed utilizing a planar laser scanner. For kidney study, cis-diamminedichloroplatinum [II] (cisplatin) was intraperitoneally administrated into mice. Results: Clear and semiquantitative fluorescent images of ROS generation in the mouse brain and kidney were obtained. Furthermore, the fluorescence intensity was stable and not affected by fading. Sodium nitroprusside induced approximately 6 times the fluorescence accumulation in the brain. Cisplatin caused renal injury in all mice, and in comparison with control mice, more than 10 times fluorescence accumulation was observed in the renal medulla with tubular necrosis and vacuolization. Conclusions: We successfully obtained ex vivo semiquantitative fluorescent images of ROS generation utilizing a planar laser scanner and DHE. This simple method is useful for ROS detection in several ROS-related animal models and would be applicable to a variety of biochemical processes.https://doi.org/10.1177/1536012118820421 |
spellingShingle | Rie Hosoi PhD Sota Sato BS Miho Shukuri PhD Yuka Fujii BS Kenichiro Todoroki PhD Yasushi Arano PhD Toshihiro Sakai PhD Osamu Inoue PhD A Simple Ex Vivo Semiquantitative Fluorescent Imaging Utilizing Planar Laser Scanner: Detection of Reactive Oxygen Species Generation in Mouse Brain and Kidney Molecular Imaging |
title | A Simple Ex Vivo Semiquantitative Fluorescent Imaging Utilizing Planar Laser Scanner: Detection of Reactive Oxygen Species Generation in Mouse Brain and Kidney |
title_full | A Simple Ex Vivo Semiquantitative Fluorescent Imaging Utilizing Planar Laser Scanner: Detection of Reactive Oxygen Species Generation in Mouse Brain and Kidney |
title_fullStr | A Simple Ex Vivo Semiquantitative Fluorescent Imaging Utilizing Planar Laser Scanner: Detection of Reactive Oxygen Species Generation in Mouse Brain and Kidney |
title_full_unstemmed | A Simple Ex Vivo Semiquantitative Fluorescent Imaging Utilizing Planar Laser Scanner: Detection of Reactive Oxygen Species Generation in Mouse Brain and Kidney |
title_short | A Simple Ex Vivo Semiquantitative Fluorescent Imaging Utilizing Planar Laser Scanner: Detection of Reactive Oxygen Species Generation in Mouse Brain and Kidney |
title_sort | simple ex vivo semiquantitative fluorescent imaging utilizing planar laser scanner detection of reactive oxygen species generation in mouse brain and kidney |
url | https://doi.org/10.1177/1536012118820421 |
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