An All-in-One Platform for On-Site Multiplex Foodborne Pathogen Detection Based on Channel-Digital Hybrid Microfluidics
Recently, significant progress has been made in the development of microdevices for point-of-care infectious disease detection. However, most microdevices only allow limited steps, such as DNA amplification on the chip, while sample preparation, such as lysis and DNA extraction, is conducted off the...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/14/1/50 |
| _version_ | 1797344478631034880 |
|---|---|
| author | Mei Xie Tianlan Chen Zongwei Cai Bo Lei Cheng Dong |
| author_facet | Mei Xie Tianlan Chen Zongwei Cai Bo Lei Cheng Dong |
| author_sort | Mei Xie |
| collection | DOAJ |
| description | Recently, significant progress has been made in the development of microdevices for point-of-care infectious disease detection. However, most microdevices only allow limited steps, such as DNA amplification on the chip, while sample preparation, such as lysis and DNA extraction, is conducted off the chip using the traditional method. In this study, an all-in-one platform was developed, which incorporated all necessary procedures for nucleic acid detection. Our on-chip DNA extraction method utilized the magnetic bead-based technology on a hybrid channel-digital microfluidics (C-DMF) microdevice. It yielded high recovery rates, varying from 88.43% to 95.83%, with pathogen concentrations of 10<sup>3</sup>–10<sup>6</sup> CFU/mL. In particular, the on-chip method exhibited significantly higher efficacy compared to the traditional off-chip manual method, for the DNA extraction of <i>E. coli</i> and <i>S. aureus</i>, representing Gram-negative and Gram-positive bacteria, respectively, at a sample concentration of 10<sup>3</sup> CFU/mL. To address the need for rapid and accessible diagnostics, colorimetric LAMP amplification was integrated into the proposed microdevice. The results were visually detectable with the naked eye, making it user-friendly for non-specialists. In addition, this platform demonstrated impressive sensitivity in simultaneously detecting common foodborne pathogens in spiked meat samples, achieving the LOD of 10<sup>2</sup>–10<sup>3</sup> CFU/mL. The entire process, from sampling to result, was fully automated and only required approximately 60 min, offering promising applicability in resource-limited and on-site testing scenarios. |
| first_indexed | 2024-03-08T11:03:12Z |
| format | Article |
| id | doaj.art-504fb0be32974027a510564d545f99b8 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-08T11:03:12Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-504fb0be32974027a510564d545f99b82024-01-26T15:23:22ZengMDPI AGBiosensors2079-63742024-01-011415010.3390/bios14010050An All-in-One Platform for On-Site Multiplex Foodborne Pathogen Detection Based on Channel-Digital Hybrid MicrofluidicsMei Xie0Tianlan Chen1Zongwei Cai2Bo Lei3Cheng Dong4Department of Life Sciences, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai 519000, ChinaDigifluidic Biotech Ltd., Zhuhai 519000, ChinaDepartment of Chemistry, Hong Kong Baptist University, Hong Kong SAR, ChinaDepartment of Life Sciences, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai 519000, ChinaSchool of Intelligent Systems Science and Engineering, Jinan University, Zhuhai 519000, ChinaRecently, significant progress has been made in the development of microdevices for point-of-care infectious disease detection. However, most microdevices only allow limited steps, such as DNA amplification on the chip, while sample preparation, such as lysis and DNA extraction, is conducted off the chip using the traditional method. In this study, an all-in-one platform was developed, which incorporated all necessary procedures for nucleic acid detection. Our on-chip DNA extraction method utilized the magnetic bead-based technology on a hybrid channel-digital microfluidics (C-DMF) microdevice. It yielded high recovery rates, varying from 88.43% to 95.83%, with pathogen concentrations of 10<sup>3</sup>–10<sup>6</sup> CFU/mL. In particular, the on-chip method exhibited significantly higher efficacy compared to the traditional off-chip manual method, for the DNA extraction of <i>E. coli</i> and <i>S. aureus</i>, representing Gram-negative and Gram-positive bacteria, respectively, at a sample concentration of 10<sup>3</sup> CFU/mL. To address the need for rapid and accessible diagnostics, colorimetric LAMP amplification was integrated into the proposed microdevice. The results were visually detectable with the naked eye, making it user-friendly for non-specialists. In addition, this platform demonstrated impressive sensitivity in simultaneously detecting common foodborne pathogens in spiked meat samples, achieving the LOD of 10<sup>2</sup>–10<sup>3</sup> CFU/mL. The entire process, from sampling to result, was fully automated and only required approximately 60 min, offering promising applicability in resource-limited and on-site testing scenarios.https://www.mdpi.com/2079-6374/14/1/50channel-digital microdevicefull integrationpathogen detectionvisually detectablepoint-of-care testing |
| spellingShingle | Mei Xie Tianlan Chen Zongwei Cai Bo Lei Cheng Dong An All-in-One Platform for On-Site Multiplex Foodborne Pathogen Detection Based on Channel-Digital Hybrid Microfluidics Biosensors channel-digital microdevice full integration pathogen detection visually detectable point-of-care testing |
| title | An All-in-One Platform for On-Site Multiplex Foodborne Pathogen Detection Based on Channel-Digital Hybrid Microfluidics |
| title_full | An All-in-One Platform for On-Site Multiplex Foodborne Pathogen Detection Based on Channel-Digital Hybrid Microfluidics |
| title_fullStr | An All-in-One Platform for On-Site Multiplex Foodborne Pathogen Detection Based on Channel-Digital Hybrid Microfluidics |
| title_full_unstemmed | An All-in-One Platform for On-Site Multiplex Foodborne Pathogen Detection Based on Channel-Digital Hybrid Microfluidics |
| title_short | An All-in-One Platform for On-Site Multiplex Foodborne Pathogen Detection Based on Channel-Digital Hybrid Microfluidics |
| title_sort | all in one platform for on site multiplex foodborne pathogen detection based on channel digital hybrid microfluidics |
| topic | channel-digital microdevice full integration pathogen detection visually detectable point-of-care testing |
| url | https://www.mdpi.com/2079-6374/14/1/50 |
| work_keys_str_mv | AT meixie anallinoneplatformforonsitemultiplexfoodbornepathogendetectionbasedonchanneldigitalhybridmicrofluidics AT tianlanchen anallinoneplatformforonsitemultiplexfoodbornepathogendetectionbasedonchanneldigitalhybridmicrofluidics AT zongweicai anallinoneplatformforonsitemultiplexfoodbornepathogendetectionbasedonchanneldigitalhybridmicrofluidics AT bolei anallinoneplatformforonsitemultiplexfoodbornepathogendetectionbasedonchanneldigitalhybridmicrofluidics AT chengdong anallinoneplatformforonsitemultiplexfoodbornepathogendetectionbasedonchanneldigitalhybridmicrofluidics AT meixie allinoneplatformforonsitemultiplexfoodbornepathogendetectionbasedonchanneldigitalhybridmicrofluidics AT tianlanchen allinoneplatformforonsitemultiplexfoodbornepathogendetectionbasedonchanneldigitalhybridmicrofluidics AT zongweicai allinoneplatformforonsitemultiplexfoodbornepathogendetectionbasedonchanneldigitalhybridmicrofluidics AT bolei allinoneplatformforonsitemultiplexfoodbornepathogendetectionbasedonchanneldigitalhybridmicrofluidics AT chengdong allinoneplatformforonsitemultiplexfoodbornepathogendetectionbasedonchanneldigitalhybridmicrofluidics |