Melatonin-induced physiology and transcriptome changes in banana seedlings under salt stress conditions
Soil salinization poses a serious threat to the ecological environment and agricultural production and is one of the most common abiotic stresses in global agricultural production. As a salt-sensitive plant, the growth, development, and production of bananas (Musa acuminata L.) are restricted by sal...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.938262/full |
| _version_ | 1811210960451403776 |
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| author | Junya Wei Jinhao Liang Debing Liu Yuewei Liu Guoyin Liu Shouxing Wei |
| author_facet | Junya Wei Jinhao Liang Debing Liu Yuewei Liu Guoyin Liu Shouxing Wei |
| author_sort | Junya Wei |
| collection | DOAJ |
| description | Soil salinization poses a serious threat to the ecological environment and agricultural production and is one of the most common abiotic stresses in global agricultural production. As a salt-sensitive plant, the growth, development, and production of bananas (Musa acuminata L.) are restricted by salt stress. Melatonin is known to improve the resistance of plants to stress. The study analyzed the effects of 100 μM melatonin on physiological and transcriptome changes in banana varieties (AAA group cv. Cavendish) under 60 mmol/l of NaCl salt stress situation. The phenotypic results showed that the application of exogenous melatonin could maintain banana plants’ health growth and alleviate the damage caused by salt stress. The physiological data show that the application of exogenous melatonin can enhance salt tolerance of banana seedlings by increasing the content of proline content and soluble protein, slowing down the degradation of chlorophyll, reducing membrane permeability and recovery of relative water content, increasing the accumulation of MDA, and enhancing antioxidant defense activity. Transcriptome sequencing showed that melatonin-induced salt tolerance of banana seedlings involved biological processes, molecular functions, and cellular components. We also found that differentially expressed genes (DEGs) are involved in a variety of metabolic pathways, including amino sugar and nucleotide sugar metabolism, phenylalanine metabolism, cyanoamino acid metabolism, starch and sucrose metabolism, and linoleic acid metabolism. These major metabolism and biosynthesis may be involved in the potential mechanism of melatonin under salt stress. Furthermore, some members of the transcription factor family, such as MYB, NAC, bHLH, and WRKY, might contribute to melatonin alleviating salt stress tolerance of the banana plant. The result laid a basis for further clarifying the salt stress resistance mechanism of bananas mediated by exogenous melatonin and provides theoretical bases to utilize melatonin to improve banana salt tolerance in the future. |
| first_indexed | 2024-04-12T05:03:28Z |
| format | Article |
| id | doaj.art-505471da73e5487bb1f32fb39f8b6d32 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T05:03:28Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-505471da73e5487bb1f32fb39f8b6d322022-12-22T03:46:55ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.938262938262Melatonin-induced physiology and transcriptome changes in banana seedlings under salt stress conditionsJunya Wei0Jinhao Liang1Debing Liu2Yuewei Liu3Guoyin Liu4Shouxing Wei5Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaApplied Science and Technology College, Hainan University, Haikou, ChinaApplied Science and Technology College, Hainan University, Haikou, ChinaApplied Science and Technology College, Hainan University, Haikou, ChinaForestry College, Hainan University, Haikou, ChinaTropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaSoil salinization poses a serious threat to the ecological environment and agricultural production and is one of the most common abiotic stresses in global agricultural production. As a salt-sensitive plant, the growth, development, and production of bananas (Musa acuminata L.) are restricted by salt stress. Melatonin is known to improve the resistance of plants to stress. The study analyzed the effects of 100 μM melatonin on physiological and transcriptome changes in banana varieties (AAA group cv. Cavendish) under 60 mmol/l of NaCl salt stress situation. The phenotypic results showed that the application of exogenous melatonin could maintain banana plants’ health growth and alleviate the damage caused by salt stress. The physiological data show that the application of exogenous melatonin can enhance salt tolerance of banana seedlings by increasing the content of proline content and soluble protein, slowing down the degradation of chlorophyll, reducing membrane permeability and recovery of relative water content, increasing the accumulation of MDA, and enhancing antioxidant defense activity. Transcriptome sequencing showed that melatonin-induced salt tolerance of banana seedlings involved biological processes, molecular functions, and cellular components. We also found that differentially expressed genes (DEGs) are involved in a variety of metabolic pathways, including amino sugar and nucleotide sugar metabolism, phenylalanine metabolism, cyanoamino acid metabolism, starch and sucrose metabolism, and linoleic acid metabolism. These major metabolism and biosynthesis may be involved in the potential mechanism of melatonin under salt stress. Furthermore, some members of the transcription factor family, such as MYB, NAC, bHLH, and WRKY, might contribute to melatonin alleviating salt stress tolerance of the banana plant. The result laid a basis for further clarifying the salt stress resistance mechanism of bananas mediated by exogenous melatonin and provides theoretical bases to utilize melatonin to improve banana salt tolerance in the future.https://www.frontiersin.org/articles/10.3389/fpls.2022.938262/fullmelatoninbananaphysiological changetranscriptomic analysissalinity |
| spellingShingle | Junya Wei Jinhao Liang Debing Liu Yuewei Liu Guoyin Liu Shouxing Wei Melatonin-induced physiology and transcriptome changes in banana seedlings under salt stress conditions Frontiers in Plant Science melatonin banana physiological change transcriptomic analysis salinity |
| title | Melatonin-induced physiology and transcriptome changes in banana seedlings under salt stress conditions |
| title_full | Melatonin-induced physiology and transcriptome changes in banana seedlings under salt stress conditions |
| title_fullStr | Melatonin-induced physiology and transcriptome changes in banana seedlings under salt stress conditions |
| title_full_unstemmed | Melatonin-induced physiology and transcriptome changes in banana seedlings under salt stress conditions |
| title_short | Melatonin-induced physiology and transcriptome changes in banana seedlings under salt stress conditions |
| title_sort | melatonin induced physiology and transcriptome changes in banana seedlings under salt stress conditions |
| topic | melatonin banana physiological change transcriptomic analysis salinity |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.938262/full |
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