Simultaneous determination of compounds in Septalen® pellets by derivative spectrophotometry
In this paper, a second-derivative spectrophotometric method of assaying Septalen ® pellets (Krka, Novo Mesto, Slovenia), which contain lidocaine 1 mg, and cetrimoniumbromide 2mg, is described. Lidocaine, 2-(diethylamino)-N-(2,6-dimethyl- phenyl)-acetamide, is a local anesthetic with pro...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2000-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2000/0352-51390006339M.pdf |
| _version_ | 1818876062867652608 |
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| author | Medenica Mirjana Ivanović Darko Malenović Anđelija |
| author_facet | Medenica Mirjana Ivanović Darko Malenović Anđelija |
| author_sort | Medenica Mirjana |
| collection | DOAJ |
| description | In this paper, a second-derivative spectrophotometric method of assaying
Septalen ® pellets (Krka, Novo Mesto, Slovenia), which contain lidocaine 1
mg, and cetrimoniumbromide 2mg, is described. Lidocaine,
2-(diethylamino)-N-(2,6-dimethyl- phenyl)-acetamide, is a local anesthetic
with pronounced antiarhythmic and anticonvulsant properties.
Cetrimoniumbromide, N,N,N-trimethyl-l-hexadecanaminium bromide, is a topical
antiseptic and cleansing agent. Lidocaine was determined at 250 nm using the
"zero crossing" technique because the signals of centrimonium bromide and the
colour ingredient are zero at this wavelength. Cetrimonium bromide was
determined by correction of the peak amplitude at 215 nm according to
lidocaine. In choosing the optimal magnitudes for the simultaneous
determination of both drugs, the following criteria were considered: (1) the
linearity of the calibration graphs as given by the correlation coefficients,
(2) the intercept, (3) the sensitivity as given by the regression
coefficient, (4) the degree of interference in the derivative measurement by
the presence of the other compound, as given by the relative percent error
and by the relative recovery, and (5) the reproducibility, as given by the
coefficient of variation, calculated by recording the second-derivative
spectra. |
| first_indexed | 2024-12-19T13:36:25Z |
| format | Article |
| id | doaj.art-5055abfaecd44569b53bf39cde77bb00 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-12-19T13:36:25Z |
| publishDate | 2000-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-5055abfaecd44569b53bf39cde77bb002022-12-21T20:19:11ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212000-01-01655-633934410.2298/JSC0006339M0352-51390006339MSimultaneous determination of compounds in Septalen® pellets by derivative spectrophotometryMedenica Mirjana0Ivanović Darko1Malenović Anđelija2Faculty of Pharmacy, Institute of Physical Chemistry, BelgradeFaculty of Pharmacy, Institute of Drug Analysis, BelgradeFaculty of Pharmacy, Institute of Drug Analysis, BelgradeIn this paper, a second-derivative spectrophotometric method of assaying Septalen ® pellets (Krka, Novo Mesto, Slovenia), which contain lidocaine 1 mg, and cetrimoniumbromide 2mg, is described. Lidocaine, 2-(diethylamino)-N-(2,6-dimethyl- phenyl)-acetamide, is a local anesthetic with pronounced antiarhythmic and anticonvulsant properties. Cetrimoniumbromide, N,N,N-trimethyl-l-hexadecanaminium bromide, is a topical antiseptic and cleansing agent. Lidocaine was determined at 250 nm using the "zero crossing" technique because the signals of centrimonium bromide and the colour ingredient are zero at this wavelength. Cetrimonium bromide was determined by correction of the peak amplitude at 215 nm according to lidocaine. In choosing the optimal magnitudes for the simultaneous determination of both drugs, the following criteria were considered: (1) the linearity of the calibration graphs as given by the correlation coefficients, (2) the intercept, (3) the sensitivity as given by the regression coefficient, (4) the degree of interference in the derivative measurement by the presence of the other compound, as given by the relative percent error and by the relative recovery, and (5) the reproducibility, as given by the coefficient of variation, calculated by recording the second-derivative spectra.http://www.doiserbia.nb.rs/img/doi/0352-5139/2000/0352-51390006339M.pdfsecond-derivative spectrophotometryzero-crossing methodmethod with correctionlidocainecetrimonium bromideseptalen® pellets |
| spellingShingle | Medenica Mirjana Ivanović Darko Malenović Anđelija Simultaneous determination of compounds in Septalen® pellets by derivative spectrophotometry Journal of the Serbian Chemical Society second-derivative spectrophotometry zero-crossing method method with correction lidocaine cetrimonium bromide septalen® pellets |
| title | Simultaneous determination of compounds in Septalen® pellets by derivative spectrophotometry |
| title_full | Simultaneous determination of compounds in Septalen® pellets by derivative spectrophotometry |
| title_fullStr | Simultaneous determination of compounds in Septalen® pellets by derivative spectrophotometry |
| title_full_unstemmed | Simultaneous determination of compounds in Septalen® pellets by derivative spectrophotometry |
| title_short | Simultaneous determination of compounds in Septalen® pellets by derivative spectrophotometry |
| title_sort | simultaneous determination of compounds in septalen r pellets by derivative spectrophotometry |
| topic | second-derivative spectrophotometry zero-crossing method method with correction lidocaine cetrimonium bromide septalen® pellets |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2000/0352-51390006339M.pdf |
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