Phenolic compounds apigenin, hesperidin and kaempferol reduce in vitro lipid accumulation in human adipocytes
Abstract Background Adipocytes derived from human mesenchymal stem cells (MSCs) are widely used to investigate adipogenesis. Taking into account both the novelty of these MSCs and the scarcity of studies focused on the effects of phenolic compounds, the aim of the present study was to analyze the ef...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-11-01
|
| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12967-017-1343-0 |
| _version_ | 1819078255681994752 |
|---|---|
| author | Saioa Gómez-Zorita Arrate Lasa Naiara Abendaño Alfredo Fernández-Quintela Andrea Mosqueda-Solís Maria Pilar Garcia-Sobreviela Jose M. Arbonés-Mainar Maria P. Portillo |
| author_facet | Saioa Gómez-Zorita Arrate Lasa Naiara Abendaño Alfredo Fernández-Quintela Andrea Mosqueda-Solís Maria Pilar Garcia-Sobreviela Jose M. Arbonés-Mainar Maria P. Portillo |
| author_sort | Saioa Gómez-Zorita |
| collection | DOAJ |
| description | Abstract Background Adipocytes derived from human mesenchymal stem cells (MSCs) are widely used to investigate adipogenesis. Taking into account both the novelty of these MSCs and the scarcity of studies focused on the effects of phenolic compounds, the aim of the present study was to analyze the effect of apigenin, hesperidin and kaempferol on pre-adipocyte and mature adipocytes derived from this type of cells. In addition, the expression of genes involved in TG accumulation was also measured. Methods Pre-adipocytes were cultured from day 0 to day 8 and mature adipocytes for 48 h with the polyphenols at doses of 1, 10 and 25 µM. Results Apigenin did not show an anti-adipogenic action. Pre-adipocytes treated with hesperidin and kaempferol showed reduced TG content at the three experimental doses. Apigenin did not modify the expression of the main adipogenic genes (c/ebpβ, c/ebpα, pparγ and srebp1c), hesperidin inhibited genes involved in the three phases of adipogenesis (c/ebpβ, srebp1c and perilipin) and kaempferol reduced c/ebpβ. In mature adipocytes, the three polyphenols reduced TG accumulation at the dose of 25 µM, but not at lower doses. All compounds increased mRNA levels of atgl. Apigenin and hesperidin decreased fasn expression. The present study shows the anti-adipogenic effect and delipidating effects of apigenin, hesperidin and kaempferol in human adipocytes derived from hMSCs. While hesperidin blocks all the stages of adipogenesis, kaempferol only inhibits the early stage. Regarding mature adipocytes, the three compounds reduce TG accumulation by activating, at least in part, lipolysis, and in the case of hesperidin and apigenin, also by reducing lipogenesis. Conclusions The present study shows for the first time the anti-adipogenic effect and delipidating effect of apigenin, hesperidin and kaempferol in human adipocytes derived from MSCs for the first time. |
| first_indexed | 2024-12-21T19:10:11Z |
| format | Article |
| id | doaj.art-505653f261a24cdcabd1f8dd3e286986 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-21T19:10:11Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-505653f261a24cdcabd1f8dd3e2869862022-12-21T18:53:13ZengBMCJournal of Translational Medicine1479-58762017-11-0115111010.1186/s12967-017-1343-0Phenolic compounds apigenin, hesperidin and kaempferol reduce in vitro lipid accumulation in human adipocytesSaioa Gómez-Zorita0Arrate Lasa1Naiara Abendaño2Alfredo Fernández-Quintela3Andrea Mosqueda-Solís4Maria Pilar Garcia-Sobreviela5Jose M. Arbonés-Mainar6Maria P. Portillo7Nutrition and Obesity Group, Department of Nutrition and Food Science and Lucio Lascaray Research Center, University of the Basque Country (UPV/EHU)Nutrition and Obesity Group, Department of Nutrition and Food Science and Lucio Lascaray Research Center, University of the Basque Country (UPV/EHU)Nutrition and Obesity Group, Department of Nutrition and Food Science and Lucio Lascaray Research Center, University of the Basque Country (UPV/EHU)Nutrition and Obesity Group, Department of Nutrition and Food Science and Lucio Lascaray Research Center, University of the Basque Country (UPV/EHU)Nutrition and Obesity Group, Department of Nutrition and Food Science and Lucio Lascaray Research Center, University of the Basque Country (UPV/EHU)Adipocyte and Fat Biology Laboratory (AdipoFat), Unidad de Investigación Traslacional, Instituto Aragonés de Ciencias de la Salud (IACS), Instituto de Investigación Sanitaria (IIS) AragónAdipocyte and Fat Biology Laboratory (AdipoFat), Unidad de Investigación Traslacional, Instituto Aragonés de Ciencias de la Salud (IACS), Instituto de Investigación Sanitaria (IIS) AragónNutrition and Obesity Group, Department of Nutrition and Food Science and Lucio Lascaray Research Center, University of the Basque Country (UPV/EHU)Abstract Background Adipocytes derived from human mesenchymal stem cells (MSCs) are widely used to investigate adipogenesis. Taking into account both the novelty of these MSCs and the scarcity of studies focused on the effects of phenolic compounds, the aim of the present study was to analyze the effect of apigenin, hesperidin and kaempferol on pre-adipocyte and mature adipocytes derived from this type of cells. In addition, the expression of genes involved in TG accumulation was also measured. Methods Pre-adipocytes were cultured from day 0 to day 8 and mature adipocytes for 48 h with the polyphenols at doses of 1, 10 and 25 µM. Results Apigenin did not show an anti-adipogenic action. Pre-adipocytes treated with hesperidin and kaempferol showed reduced TG content at the three experimental doses. Apigenin did not modify the expression of the main adipogenic genes (c/ebpβ, c/ebpα, pparγ and srebp1c), hesperidin inhibited genes involved in the three phases of adipogenesis (c/ebpβ, srebp1c and perilipin) and kaempferol reduced c/ebpβ. In mature adipocytes, the three polyphenols reduced TG accumulation at the dose of 25 µM, but not at lower doses. All compounds increased mRNA levels of atgl. Apigenin and hesperidin decreased fasn expression. The present study shows the anti-adipogenic effect and delipidating effects of apigenin, hesperidin and kaempferol in human adipocytes derived from hMSCs. While hesperidin blocks all the stages of adipogenesis, kaempferol only inhibits the early stage. Regarding mature adipocytes, the three compounds reduce TG accumulation by activating, at least in part, lipolysis, and in the case of hesperidin and apigenin, also by reducing lipogenesis. Conclusions The present study shows for the first time the anti-adipogenic effect and delipidating effect of apigenin, hesperidin and kaempferol in human adipocytes derived from MSCs for the first time.http://link.springer.com/article/10.1186/s12967-017-1343-0AdipocytesApigeninHesperidinKaempferolObesityHuman mesenchymal stem cells |
| spellingShingle | Saioa Gómez-Zorita Arrate Lasa Naiara Abendaño Alfredo Fernández-Quintela Andrea Mosqueda-Solís Maria Pilar Garcia-Sobreviela Jose M. Arbonés-Mainar Maria P. Portillo Phenolic compounds apigenin, hesperidin and kaempferol reduce in vitro lipid accumulation in human adipocytes Journal of Translational Medicine Adipocytes Apigenin Hesperidin Kaempferol Obesity Human mesenchymal stem cells |
| title | Phenolic compounds apigenin, hesperidin and kaempferol reduce in vitro lipid accumulation in human adipocytes |
| title_full | Phenolic compounds apigenin, hesperidin and kaempferol reduce in vitro lipid accumulation in human adipocytes |
| title_fullStr | Phenolic compounds apigenin, hesperidin and kaempferol reduce in vitro lipid accumulation in human adipocytes |
| title_full_unstemmed | Phenolic compounds apigenin, hesperidin and kaempferol reduce in vitro lipid accumulation in human adipocytes |
| title_short | Phenolic compounds apigenin, hesperidin and kaempferol reduce in vitro lipid accumulation in human adipocytes |
| title_sort | phenolic compounds apigenin hesperidin and kaempferol reduce in vitro lipid accumulation in human adipocytes |
| topic | Adipocytes Apigenin Hesperidin Kaempferol Obesity Human mesenchymal stem cells |
| url | http://link.springer.com/article/10.1186/s12967-017-1343-0 |
| work_keys_str_mv | AT saioagomezzorita phenoliccompoundsapigeninhesperidinandkaempferolreduceinvitrolipidaccumulationinhumanadipocytes AT arratelasa phenoliccompoundsapigeninhesperidinandkaempferolreduceinvitrolipidaccumulationinhumanadipocytes AT naiaraabendano phenoliccompoundsapigeninhesperidinandkaempferolreduceinvitrolipidaccumulationinhumanadipocytes AT alfredofernandezquintela phenoliccompoundsapigeninhesperidinandkaempferolreduceinvitrolipidaccumulationinhumanadipocytes AT andreamosquedasolis phenoliccompoundsapigeninhesperidinandkaempferolreduceinvitrolipidaccumulationinhumanadipocytes AT mariapilargarciasobreviela phenoliccompoundsapigeninhesperidinandkaempferolreduceinvitrolipidaccumulationinhumanadipocytes AT josemarbonesmainar phenoliccompoundsapigeninhesperidinandkaempferolreduceinvitrolipidaccumulationinhumanadipocytes AT mariapportillo phenoliccompoundsapigeninhesperidinandkaempferolreduceinvitrolipidaccumulationinhumanadipocytes |