Nanospheres Loaded with Curcumin Improve the Bioactivity of Umbilical Cord Blood-Mesenchymal Stem Cells via c-Src Activation during the Skin Wound Healing Process
Curcumin, a hydrophobic polyphenol derived from turmeric, has been used a food additive and as a herbal medicine for the treatment of various diseases, but the clinical application of curcumin is restricted by its poor aqueous solubility and its low permeability and bioavailability levels. In the pr...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-06-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/9/6/1467 |
| _version_ | 1797565224703754240 |
|---|---|
| author | Do-Wan Kim Chang-Hyung Choi Jong Pil Park Sei-Jung Lee |
| author_facet | Do-Wan Kim Chang-Hyung Choi Jong Pil Park Sei-Jung Lee |
| author_sort | Do-Wan Kim |
| collection | DOAJ |
| description | Curcumin, a hydrophobic polyphenol derived from turmeric, has been used a food additive and as a herbal medicine for the treatment of various diseases, but the clinical application of curcumin is restricted by its poor aqueous solubility and its low permeability and bioavailability levels. In the present study, we investigate the functional role of a nanosphere loaded with curcumin (CN) in the promotion of the motility of human mesenchymal stem cells (MSCs) during the skin wound healing process. CN significantly increased the motility of umbilical cord blood (UCB)-MSCs and showed 10,000-fold greater migration efficacy than curcumin. CN stimulated the phosphorylation of c-Src and protein kinase C which are responsible for the distinctive activation of the MAPKs. Interestingly, CN significantly induced the expression levels of α-actinin-1, profilin-1 and filamentous-actin, as regulated by the phosphorylation of nuclear factor-kappa B during its promotion of cell migration. In a mouse skin excisional wound model, we found that transplantation of UCB-MSCs pre-treated with CN enhanced wound closure, granulation, and re-epithelialization at mouse skin wound sites. These results indicate that CN is a functional agent that promotes the mobilization of UCB-MSCs for cutaneous wound repair. |
| first_indexed | 2024-03-10T19:09:04Z |
| format | Article |
| id | doaj.art-505872a0b6004f0d9f0a9b2d2907e511 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T19:09:04Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-505872a0b6004f0d9f0a9b2d2907e5112023-11-20T03:54:58ZengMDPI AGCells2073-44092020-06-0196146710.3390/cells9061467Nanospheres Loaded with Curcumin Improve the Bioactivity of Umbilical Cord Blood-Mesenchymal Stem Cells via c-Src Activation during the Skin Wound Healing ProcessDo-Wan Kim0Chang-Hyung Choi1Jong Pil Park2Sei-Jung Lee3Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan 38610, KoreaDivision of Cosmetic Science and Technology, Daegu Haany University, Gyeongsan 38610, KoreaDepartment of Food Science and Technology, Chung-Ang University, Anseong 17546, KoreaDepartment of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan 38610, KoreaCurcumin, a hydrophobic polyphenol derived from turmeric, has been used a food additive and as a herbal medicine for the treatment of various diseases, but the clinical application of curcumin is restricted by its poor aqueous solubility and its low permeability and bioavailability levels. In the present study, we investigate the functional role of a nanosphere loaded with curcumin (CN) in the promotion of the motility of human mesenchymal stem cells (MSCs) during the skin wound healing process. CN significantly increased the motility of umbilical cord blood (UCB)-MSCs and showed 10,000-fold greater migration efficacy than curcumin. CN stimulated the phosphorylation of c-Src and protein kinase C which are responsible for the distinctive activation of the MAPKs. Interestingly, CN significantly induced the expression levels of α-actinin-1, profilin-1 and filamentous-actin, as regulated by the phosphorylation of nuclear factor-kappa B during its promotion of cell migration. In a mouse skin excisional wound model, we found that transplantation of UCB-MSCs pre-treated with CN enhanced wound closure, granulation, and re-epithelialization at mouse skin wound sites. These results indicate that CN is a functional agent that promotes the mobilization of UCB-MSCs for cutaneous wound repair.https://www.mdpi.com/2073-4409/9/6/1467curcumin nanospheresUCB-MSCsmotilityc-Srcwound repair |
| spellingShingle | Do-Wan Kim Chang-Hyung Choi Jong Pil Park Sei-Jung Lee Nanospheres Loaded with Curcumin Improve the Bioactivity of Umbilical Cord Blood-Mesenchymal Stem Cells via c-Src Activation during the Skin Wound Healing Process Cells curcumin nanospheres UCB-MSCs motility c-Src wound repair |
| title | Nanospheres Loaded with Curcumin Improve the Bioactivity of Umbilical Cord Blood-Mesenchymal Stem Cells via c-Src Activation during the Skin Wound Healing Process |
| title_full | Nanospheres Loaded with Curcumin Improve the Bioactivity of Umbilical Cord Blood-Mesenchymal Stem Cells via c-Src Activation during the Skin Wound Healing Process |
| title_fullStr | Nanospheres Loaded with Curcumin Improve the Bioactivity of Umbilical Cord Blood-Mesenchymal Stem Cells via c-Src Activation during the Skin Wound Healing Process |
| title_full_unstemmed | Nanospheres Loaded with Curcumin Improve the Bioactivity of Umbilical Cord Blood-Mesenchymal Stem Cells via c-Src Activation during the Skin Wound Healing Process |
| title_short | Nanospheres Loaded with Curcumin Improve the Bioactivity of Umbilical Cord Blood-Mesenchymal Stem Cells via c-Src Activation during the Skin Wound Healing Process |
| title_sort | nanospheres loaded with curcumin improve the bioactivity of umbilical cord blood mesenchymal stem cells via c src activation during the skin wound healing process |
| topic | curcumin nanospheres UCB-MSCs motility c-Src wound repair |
| url | https://www.mdpi.com/2073-4409/9/6/1467 |
| work_keys_str_mv | AT dowankim nanospheresloadedwithcurcuminimprovethebioactivityofumbilicalcordbloodmesenchymalstemcellsviacsrcactivationduringtheskinwoundhealingprocess AT changhyungchoi nanospheresloadedwithcurcuminimprovethebioactivityofumbilicalcordbloodmesenchymalstemcellsviacsrcactivationduringtheskinwoundhealingprocess AT jongpilpark nanospheresloadedwithcurcuminimprovethebioactivityofumbilicalcordbloodmesenchymalstemcellsviacsrcactivationduringtheskinwoundhealingprocess AT seijunglee nanospheresloadedwithcurcuminimprovethebioactivityofumbilicalcordbloodmesenchymalstemcellsviacsrcactivationduringtheskinwoundhealingprocess |