Self-snapping hydrogel-based electroactive microchannels as nerve guidance conduits
Peripheral nerve regeneration with large defects needs innovative design of nerve guidance conduits (NGCs) which possess anisotropic guidance, electrical induction and right mechanical properties in one. Herein, we present, for the first time, facile fabrication and efficient neural differentiation...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
|
| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006422002356 |
| _version_ | 1798030785514569728 |
|---|---|
| author | Jordi Amagat Yingchun Su Frederik Høbjerg Svejsø Alice Le Friec Steffan Møller Sønderskov Mingdong Dong Ying Fang Menglin Chen |
| author_facet | Jordi Amagat Yingchun Su Frederik Høbjerg Svejsø Alice Le Friec Steffan Møller Sønderskov Mingdong Dong Ying Fang Menglin Chen |
| author_sort | Jordi Amagat |
| collection | DOAJ |
| description | Peripheral nerve regeneration with large defects needs innovative design of nerve guidance conduits (NGCs) which possess anisotropic guidance, electrical induction and right mechanical properties in one. Herein, we present, for the first time, facile fabrication and efficient neural differentiation guidance of anisotropic, conductive, self-snapping, hydrogel-based NGCs. The hydrogels were fabricated via crosslinking of graphitic carbon nitride (g-C3N4) upon exposure with blue light, incorporated with graphene oxide (GO). Incorporation of GO and in situ reduction greatly enhanced surface charges, while decayed light penetration endowed the hydrogel with an intriguing self-snapping feature by the virtue of a crosslinking gradient. The hydrogels were in the optimal mechanical stiffness range for peripheral nerve regeneration and supported normal viability and proliferation of neural cells. The PC12 cells differentiated on the electroactive g-C3N4 H/rGO3 (3 mg/mL GO loading) hydrogel presented 47% longer neurite length than that of the pristine g-C3N4 H hydrogel. Furthermore, the NGC with aligned microchannels was successfully fabricated using sacrificial melt electrowriting (MEW) moulding, the anisotropic microchannels of the 10 μm width showed optimal neurite guidance. Such anisotropic, electroactive, self-snapping NGCs may possess great potential for repairing peripheral nerve injuries. |
| first_indexed | 2024-04-11T19:46:57Z |
| format | Article |
| id | doaj.art-5058d98e1de74f29b96e57fac8dd87ef |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-04-11T19:46:57Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-5058d98e1de74f29b96e57fac8dd87ef2022-12-22T04:06:27ZengElsevierMaterials Today Bio2590-00642022-12-0116100437Self-snapping hydrogel-based electroactive microchannels as nerve guidance conduitsJordi Amagat0Yingchun Su1Frederik Høbjerg Svejsø2Alice Le Friec3Steffan Møller Sønderskov4Mingdong Dong5Ying Fang6Menglin Chen7Department of Biological and Chemical Engineering, Aarhus University, Denmark; Sino-Danish College (SDC), University of Chinese Academy of Sciences, Beijing, 101400, ChinaDepartment of Biological and Chemical Engineering, Aarhus University, Denmark; School of Electrical Engineering and Computer Science, KTH Royal Institute of Technology, Electrum 229, 16440, Kista, SwedenDepartment of Biological and Chemical Engineering, Aarhus University, DenmarkDepartment of Biological and Chemical Engineering, Aarhus University, DenmarkInterdisciplinary Nanoscience Center, INANO, Aarhus University, DenmarkInterdisciplinary Nanoscience Center, INANO, Aarhus University, DenmarkCAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, 100190, China; CAS Center for Excellence in Brain Science and Intelligence Technology, Institute of Neuroscience, Chinese Academy of Sciences, Shanghai 200031, ChinaDepartment of Biological and Chemical Engineering, Aarhus University, Denmark; Interdisciplinary Nanoscience Center, INANO, Aarhus University, Denmark; Corresponding author. Aarhus University, Universitetsbyen 36, 8000, Aarhus C, Denmark.Peripheral nerve regeneration with large defects needs innovative design of nerve guidance conduits (NGCs) which possess anisotropic guidance, electrical induction and right mechanical properties in one. Herein, we present, for the first time, facile fabrication and efficient neural differentiation guidance of anisotropic, conductive, self-snapping, hydrogel-based NGCs. The hydrogels were fabricated via crosslinking of graphitic carbon nitride (g-C3N4) upon exposure with blue light, incorporated with graphene oxide (GO). Incorporation of GO and in situ reduction greatly enhanced surface charges, while decayed light penetration endowed the hydrogel with an intriguing self-snapping feature by the virtue of a crosslinking gradient. The hydrogels were in the optimal mechanical stiffness range for peripheral nerve regeneration and supported normal viability and proliferation of neural cells. The PC12 cells differentiated on the electroactive g-C3N4 H/rGO3 (3 mg/mL GO loading) hydrogel presented 47% longer neurite length than that of the pristine g-C3N4 H hydrogel. Furthermore, the NGC with aligned microchannels was successfully fabricated using sacrificial melt electrowriting (MEW) moulding, the anisotropic microchannels of the 10 μm width showed optimal neurite guidance. Such anisotropic, electroactive, self-snapping NGCs may possess great potential for repairing peripheral nerve injuries.http://www.sciencedirect.com/science/article/pii/S2590006422002356HydrogelAnisotropicGraphitic carbon nitrideGraphene oxideSnappingNerve guidance conduit |
| spellingShingle | Jordi Amagat Yingchun Su Frederik Høbjerg Svejsø Alice Le Friec Steffan Møller Sønderskov Mingdong Dong Ying Fang Menglin Chen Self-snapping hydrogel-based electroactive microchannels as nerve guidance conduits Materials Today Bio Hydrogel Anisotropic Graphitic carbon nitride Graphene oxide Snapping Nerve guidance conduit |
| title | Self-snapping hydrogel-based electroactive microchannels as nerve guidance conduits |
| title_full | Self-snapping hydrogel-based electroactive microchannels as nerve guidance conduits |
| title_fullStr | Self-snapping hydrogel-based electroactive microchannels as nerve guidance conduits |
| title_full_unstemmed | Self-snapping hydrogel-based electroactive microchannels as nerve guidance conduits |
| title_short | Self-snapping hydrogel-based electroactive microchannels as nerve guidance conduits |
| title_sort | self snapping hydrogel based electroactive microchannels as nerve guidance conduits |
| topic | Hydrogel Anisotropic Graphitic carbon nitride Graphene oxide Snapping Nerve guidance conduit |
| url | http://www.sciencedirect.com/science/article/pii/S2590006422002356 |
| work_keys_str_mv | AT jordiamagat selfsnappinghydrogelbasedelectroactivemicrochannelsasnerveguidanceconduits AT yingchunsu selfsnappinghydrogelbasedelectroactivemicrochannelsasnerveguidanceconduits AT frederikhøbjergsvejsø selfsnappinghydrogelbasedelectroactivemicrochannelsasnerveguidanceconduits AT alicelefriec selfsnappinghydrogelbasedelectroactivemicrochannelsasnerveguidanceconduits AT steffanmøllersønderskov selfsnappinghydrogelbasedelectroactivemicrochannelsasnerveguidanceconduits AT mingdongdong selfsnappinghydrogelbasedelectroactivemicrochannelsasnerveguidanceconduits AT yingfang selfsnappinghydrogelbasedelectroactivemicrochannelsasnerveguidanceconduits AT menglinchen selfsnappinghydrogelbasedelectroactivemicrochannelsasnerveguidanceconduits |