Insights into the early transcriptomic response against watermelon mosaic virus in melon
Abstract Background Watermelon mosaic virus (WMV) is one of the most prevalent viruses affecting melon worldwide. Recessive resistance to WMV in melon has previously been reported in the African accession TGR-1551. Moreover, the genomic regions associated to the resistance have also been described....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-024-04745-x |
| _version_ | 1797349990784303104 |
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| author | María López-Martín Javier Montero-Pau Guillem Ylla María Luisa Gómez-Guillamón Belén Picó Ana Pérez-de-Castro |
| author_facet | María López-Martín Javier Montero-Pau Guillem Ylla María Luisa Gómez-Guillamón Belén Picó Ana Pérez-de-Castro |
| author_sort | María López-Martín |
| collection | DOAJ |
| description | Abstract Background Watermelon mosaic virus (WMV) is one of the most prevalent viruses affecting melon worldwide. Recessive resistance to WMV in melon has previously been reported in the African accession TGR-1551. Moreover, the genomic regions associated to the resistance have also been described. Nevertheless, the transcriptomic response that might infer the resistance to this potyvirus has not been explored. Results We have performed a comparative transcriptomic analysis using mock and WMV-inoculated plants of the susceptible cultivar “Bola de oro” (BO) and a resistant RIL (Recombinant inbred line) derived from the initial cross between “TGR-1551” and BO. In total, 616 genes were identified as differentially expressed and the weighted gene co-expression network analysis (WGCNA) detected 19 gene clusters (GCs), of which 7 were differentially expressed for the genotype x treatment interaction term. SNPs with a predicted high impact on the protein function were detected within the coding regions of most of the detected DEGs. Moreover, 3 and 16 DEGs were detected within the QTL regions previously described in chromosomes 11 and 5, respectively. In addition to these two specific genomic regions, we also observde large transcriptomic changes from genes spread across the genome in the resistant plants in response to the virus infection. This early response against WMV implied genes involved in plant-pathogen interaction, plant hormone signal transduction, the MAPK signaling pathway or ubiquitin mediated proteolysis, in detriment to the photosynthetic and basal metabolites pathways. Moreover, the gene MELO3C021395, which coded a mediator of RNA polymerase II transcription subunit 33A (MED33A), has been proposed as the candidate gene located on chromosome 11 conferring resistance to WMV. Conclusions The comparative transcriptomic analysis presented here showed that, even though the resistance to WMV in TGR-1551 has a recessive nature, it triggers an active defense response at a transcriptomic level, which involves broad-spectrum resistance mechanisms. Thus, this study represents a step forward on our understanding of the mechanisms underlaying WMV resistance in melon. In addition, it sheds light into a broader topic on the mechanisms of recessive resistances. |
| first_indexed | 2024-03-08T12:39:18Z |
| format | Article |
| id | doaj.art-50591c1df4ce49e0b837bba3369bb0f8 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-08T12:39:18Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-50591c1df4ce49e0b837bba3369bb0f82024-01-21T12:16:14ZengBMCBMC Plant Biology1471-22292024-01-0124112010.1186/s12870-024-04745-xInsights into the early transcriptomic response against watermelon mosaic virus in melonMaría López-Martín0Javier Montero-Pau1Guillem Ylla2María Luisa Gómez-Guillamón3Belén Picó4Ana Pérez-de-Castro5COMAV, Instituto de Conservación y Mejora de la Agrodiversidad Valenciana, Universitat Politècnica de ValènciaInstituto Cavanilles de biodiversidad y la biología evolutiva (ICBIBE), Universidad de ValenciaLaboratory of Bioinformatics and Genome Biology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian UniversityInstituto de Hortofruticultura Subtropical y Mediterránea La Mayora, CSIC-UMACOMAV, Instituto de Conservación y Mejora de la Agrodiversidad Valenciana, Universitat Politècnica de ValènciaCOMAV, Instituto de Conservación y Mejora de la Agrodiversidad Valenciana, Universitat Politècnica de ValènciaAbstract Background Watermelon mosaic virus (WMV) is one of the most prevalent viruses affecting melon worldwide. Recessive resistance to WMV in melon has previously been reported in the African accession TGR-1551. Moreover, the genomic regions associated to the resistance have also been described. Nevertheless, the transcriptomic response that might infer the resistance to this potyvirus has not been explored. Results We have performed a comparative transcriptomic analysis using mock and WMV-inoculated plants of the susceptible cultivar “Bola de oro” (BO) and a resistant RIL (Recombinant inbred line) derived from the initial cross between “TGR-1551” and BO. In total, 616 genes were identified as differentially expressed and the weighted gene co-expression network analysis (WGCNA) detected 19 gene clusters (GCs), of which 7 were differentially expressed for the genotype x treatment interaction term. SNPs with a predicted high impact on the protein function were detected within the coding regions of most of the detected DEGs. Moreover, 3 and 16 DEGs were detected within the QTL regions previously described in chromosomes 11 and 5, respectively. In addition to these two specific genomic regions, we also observde large transcriptomic changes from genes spread across the genome in the resistant plants in response to the virus infection. This early response against WMV implied genes involved in plant-pathogen interaction, plant hormone signal transduction, the MAPK signaling pathway or ubiquitin mediated proteolysis, in detriment to the photosynthetic and basal metabolites pathways. Moreover, the gene MELO3C021395, which coded a mediator of RNA polymerase II transcription subunit 33A (MED33A), has been proposed as the candidate gene located on chromosome 11 conferring resistance to WMV. Conclusions The comparative transcriptomic analysis presented here showed that, even though the resistance to WMV in TGR-1551 has a recessive nature, it triggers an active defense response at a transcriptomic level, which involves broad-spectrum resistance mechanisms. Thus, this study represents a step forward on our understanding of the mechanisms underlaying WMV resistance in melon. In addition, it sheds light into a broader topic on the mechanisms of recessive resistances.https://doi.org/10.1186/s12870-024-04745-xWMVMelonTGR-1551RNA-seqWGCNASNPeff |
| spellingShingle | María López-Martín Javier Montero-Pau Guillem Ylla María Luisa Gómez-Guillamón Belén Picó Ana Pérez-de-Castro Insights into the early transcriptomic response against watermelon mosaic virus in melon BMC Plant Biology WMV Melon TGR-1551 RNA-seq WGCNA SNPeff |
| title | Insights into the early transcriptomic response against watermelon mosaic virus in melon |
| title_full | Insights into the early transcriptomic response against watermelon mosaic virus in melon |
| title_fullStr | Insights into the early transcriptomic response against watermelon mosaic virus in melon |
| title_full_unstemmed | Insights into the early transcriptomic response against watermelon mosaic virus in melon |
| title_short | Insights into the early transcriptomic response against watermelon mosaic virus in melon |
| title_sort | insights into the early transcriptomic response against watermelon mosaic virus in melon |
| topic | WMV Melon TGR-1551 RNA-seq WGCNA SNPeff |
| url | https://doi.org/10.1186/s12870-024-04745-x |
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