LncRNA Jpx induces Xist expression in mice using both trans and cis mechanisms.
Mammalian X chromosome dosage compensation balances X-linked gene products between sexes and is coordinated by the long noncoding RNA (lncRNA) Xist. Multiple cis and trans-acting factors modulate Xist expression; however, the primary competence factor responsible for activating Xist remains a subjec...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-05-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC5957434?pdf=render |
| _version_ | 1818315283763298304 |
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| author | Sarah Carmona Benjamin Lin Tristan Chou Katti Arroyo Sha Sun |
| author_facet | Sarah Carmona Benjamin Lin Tristan Chou Katti Arroyo Sha Sun |
| author_sort | Sarah Carmona |
| collection | DOAJ |
| description | Mammalian X chromosome dosage compensation balances X-linked gene products between sexes and is coordinated by the long noncoding RNA (lncRNA) Xist. Multiple cis and trans-acting factors modulate Xist expression; however, the primary competence factor responsible for activating Xist remains a subject of dispute. The lncRNA Jpx is a proposed competence factor, yet it remains unknown if Jpx is sufficient to activate Xist expression in mice. Here, we utilize a novel transgenic mouse system to demonstrate a dose-dependent relationship between Jpx copy number and ensuing Jpx and Xist expression. By localizing transcripts of Jpx and Xist using RNA Fluorescence in situ Hybridization (FISH) in mouse embryonic cells, we provide evidence of Jpx acting in both trans and cis to activate Xist. Our data contribute functional and mechanistic insight for lncRNA activity in mice, and argue that Jpx is a competence factor for Xist activation in vivo. |
| first_indexed | 2024-12-13T09:03:05Z |
| format | Article |
| id | doaj.art-505979ea3c1b4b00bbb707d2bc2c67bf |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-13T09:03:05Z |
| publishDate | 2018-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-505979ea3c1b4b00bbb707d2bc2c67bf2022-12-21T23:53:08ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042018-05-01145e100737810.1371/journal.pgen.1007378LncRNA Jpx induces Xist expression in mice using both trans and cis mechanisms.Sarah CarmonaBenjamin LinTristan ChouKatti ArroyoSha SunMammalian X chromosome dosage compensation balances X-linked gene products between sexes and is coordinated by the long noncoding RNA (lncRNA) Xist. Multiple cis and trans-acting factors modulate Xist expression; however, the primary competence factor responsible for activating Xist remains a subject of dispute. The lncRNA Jpx is a proposed competence factor, yet it remains unknown if Jpx is sufficient to activate Xist expression in mice. Here, we utilize a novel transgenic mouse system to demonstrate a dose-dependent relationship between Jpx copy number and ensuing Jpx and Xist expression. By localizing transcripts of Jpx and Xist using RNA Fluorescence in situ Hybridization (FISH) in mouse embryonic cells, we provide evidence of Jpx acting in both trans and cis to activate Xist. Our data contribute functional and mechanistic insight for lncRNA activity in mice, and argue that Jpx is a competence factor for Xist activation in vivo.http://europepmc.org/articles/PMC5957434?pdf=render |
| spellingShingle | Sarah Carmona Benjamin Lin Tristan Chou Katti Arroyo Sha Sun LncRNA Jpx induces Xist expression in mice using both trans and cis mechanisms. PLoS Genetics |
| title | LncRNA Jpx induces Xist expression in mice using both trans and cis mechanisms. |
| title_full | LncRNA Jpx induces Xist expression in mice using both trans and cis mechanisms. |
| title_fullStr | LncRNA Jpx induces Xist expression in mice using both trans and cis mechanisms. |
| title_full_unstemmed | LncRNA Jpx induces Xist expression in mice using both trans and cis mechanisms. |
| title_short | LncRNA Jpx induces Xist expression in mice using both trans and cis mechanisms. |
| title_sort | lncrna jpx induces xist expression in mice using both trans and cis mechanisms |
| url | http://europepmc.org/articles/PMC5957434?pdf=render |
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