Field evaluations of four SARS-CoV-2 rapid antigen tests during SARS-CoV-2 Delta variant wave in South Africa
Abstract Background Rapid antigen tests detecting SARS-CoV-2 were shown to be a useful tool in managing the COVID-19 pandemic. Here, we report on the results of a prospective diagnostic accuracy study of four SARS-CoV-2 rapid antigen tests in a South African setting. Methods Rapid antigen test evalu...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | Diagnostic and Prognostic Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s41512-023-00151-3 |
| _version_ | 1797769298121326592 |
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| author | Natasha Samsunder Gila Lustig Slindile Ngubane Thando Glory Maseko Santhuri Rambaran Sinaye Ngcapu Stanley Nzuzo Magini Lara Lewis Cherie Cawood Ayesha B. M. Kharsany Quarraisha Abdool Karim Salim Abdool Karim Kogieleum Naidoo Aida Sivro |
| author_facet | Natasha Samsunder Gila Lustig Slindile Ngubane Thando Glory Maseko Santhuri Rambaran Sinaye Ngcapu Stanley Nzuzo Magini Lara Lewis Cherie Cawood Ayesha B. M. Kharsany Quarraisha Abdool Karim Salim Abdool Karim Kogieleum Naidoo Aida Sivro |
| author_sort | Natasha Samsunder |
| collection | DOAJ |
| description | Abstract Background Rapid antigen tests detecting SARS-CoV-2 were shown to be a useful tool in managing the COVID-19 pandemic. Here, we report on the results of a prospective diagnostic accuracy study of four SARS-CoV-2 rapid antigen tests in a South African setting. Methods Rapid antigen test evaluations were performed through drive-through testing centres in Durban, South Africa, from July to December 2021. Two evaluation studies were performed: nasal Panbio COVID-19 Ag Rapid Test Device (Abbott) was evaluated in parallel with the nasopharyngeal Espline SARS-CoV-2 Ag test (Fujirebio), followed by the evaluation of nasal RightSign COVID-19 Antigen Rapid test Cassette (Hangzhou Biotest Biotech) in parallel with the nasopharyngeal STANDARD Q COVID-19 Ag test (SD Biosensor). The Abbott RealTime SARS-CoV-2 assay was used as a reference test. Results Evaluation of Panbio and Espline Ag tests was performed on 494 samples (31% positivity), while the evaluation of Standard Q and RightTest Ag tests was performed on 539 samples (13.17% positivity). The overall sensitivity for all four tests ranged between 60 and 72% with excellent specificity values (> 98%). Sensitivity increased to > 80% in all tests in samples with cycle number value < 20. All four tests performed best in samples from patients presenting within the first week of symptom onset. Conclusions All four evaluated tests detected a majority of the cases within the first week of symptom onset with high viral load. |
| first_indexed | 2024-03-12T21:05:58Z |
| format | Article |
| id | doaj.art-505b48ee824d48f691d115c05059f399 |
| institution | Directory Open Access Journal |
| issn | 2397-7523 |
| language | English |
| last_indexed | 2024-03-12T21:05:58Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Diagnostic and Prognostic Research |
| spelling | doaj.art-505b48ee824d48f691d115c05059f3992023-07-30T11:27:00ZengBMCDiagnostic and Prognostic Research2397-75232023-07-01711610.1186/s41512-023-00151-3Field evaluations of four SARS-CoV-2 rapid antigen tests during SARS-CoV-2 Delta variant wave in South AfricaNatasha Samsunder0Gila Lustig1Slindile Ngubane2Thando Glory Maseko3Santhuri Rambaran4Sinaye Ngcapu5Stanley Nzuzo Magini6Lara Lewis7Cherie Cawood8Ayesha B. M. Kharsany9Quarraisha Abdool Karim10Salim Abdool Karim11Kogieleum Naidoo12Aida Sivro13Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Epicentre AIDS Risk Management (Pty) LimitedCentre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Centre for the AIDS Programme of Research in South Africa (CAPRISA)Abstract Background Rapid antigen tests detecting SARS-CoV-2 were shown to be a useful tool in managing the COVID-19 pandemic. Here, we report on the results of a prospective diagnostic accuracy study of four SARS-CoV-2 rapid antigen tests in a South African setting. Methods Rapid antigen test evaluations were performed through drive-through testing centres in Durban, South Africa, from July to December 2021. Two evaluation studies were performed: nasal Panbio COVID-19 Ag Rapid Test Device (Abbott) was evaluated in parallel with the nasopharyngeal Espline SARS-CoV-2 Ag test (Fujirebio), followed by the evaluation of nasal RightSign COVID-19 Antigen Rapid test Cassette (Hangzhou Biotest Biotech) in parallel with the nasopharyngeal STANDARD Q COVID-19 Ag test (SD Biosensor). The Abbott RealTime SARS-CoV-2 assay was used as a reference test. Results Evaluation of Panbio and Espline Ag tests was performed on 494 samples (31% positivity), while the evaluation of Standard Q and RightTest Ag tests was performed on 539 samples (13.17% positivity). The overall sensitivity for all four tests ranged between 60 and 72% with excellent specificity values (> 98%). Sensitivity increased to > 80% in all tests in samples with cycle number value < 20. All four tests performed best in samples from patients presenting within the first week of symptom onset. Conclusions All four evaluated tests detected a majority of the cases within the first week of symptom onset with high viral load.https://doi.org/10.1186/s41512-023-00151-3COVID-19SARS-CoV-2Antigen rapid diagnostic testPerformance evaluation |
| spellingShingle | Natasha Samsunder Gila Lustig Slindile Ngubane Thando Glory Maseko Santhuri Rambaran Sinaye Ngcapu Stanley Nzuzo Magini Lara Lewis Cherie Cawood Ayesha B. M. Kharsany Quarraisha Abdool Karim Salim Abdool Karim Kogieleum Naidoo Aida Sivro Field evaluations of four SARS-CoV-2 rapid antigen tests during SARS-CoV-2 Delta variant wave in South Africa Diagnostic and Prognostic Research COVID-19 SARS-CoV-2 Antigen rapid diagnostic test Performance evaluation |
| title | Field evaluations of four SARS-CoV-2 rapid antigen tests during SARS-CoV-2 Delta variant wave in South Africa |
| title_full | Field evaluations of four SARS-CoV-2 rapid antigen tests during SARS-CoV-2 Delta variant wave in South Africa |
| title_fullStr | Field evaluations of four SARS-CoV-2 rapid antigen tests during SARS-CoV-2 Delta variant wave in South Africa |
| title_full_unstemmed | Field evaluations of four SARS-CoV-2 rapid antigen tests during SARS-CoV-2 Delta variant wave in South Africa |
| title_short | Field evaluations of four SARS-CoV-2 rapid antigen tests during SARS-CoV-2 Delta variant wave in South Africa |
| title_sort | field evaluations of four sars cov 2 rapid antigen tests during sars cov 2 delta variant wave in south africa |
| topic | COVID-19 SARS-CoV-2 Antigen rapid diagnostic test Performance evaluation |
| url | https://doi.org/10.1186/s41512-023-00151-3 |
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