The effect of controlled ovarian hyperstimulation on ovarian reserve via PTEN pathway
This study was carried out to investigate whether repeated controlled ovarian hyperstimulation (COH) affects ovarian reserve. For this reason, we aimed to show possible changes in the expression of PTEN and FOXO3, which are involved in preserving the over-reserve, after applying the COH protocol met...
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| Language: | English |
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Bioscientifica
2022-09-01
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| Series: | Reproduction and Fertility |
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| Online Access: | https://raf.bioscientifica.com/view/journals/raf/3/3/RAF-21-0075.xml |
| _version_ | 1818023354289881088 |
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| author | Perihan Sezginer Cigdem Elmas Fatma Yıldız |
| author_facet | Perihan Sezginer Cigdem Elmas Fatma Yıldız |
| author_sort | Perihan Sezginer |
| collection | DOAJ |
| description | This study was carried out to investigate whether repeated controlled ovarian hyperstimulation (COH) affects ovarian reserve. For this reason, we aimed to show possible changes in the expression of PTEN and FOXO3, which are involved in preserving the over-reserve, after applying the COH protocol methods. For this purpose, 18 young Wistar albino female rats (8 weeks old) were randomly assigned as group 1 (control), group 2, and group 3 as 6 subjects in each group. Experimental groups were treated with 10 IU/0.1 mL pregnant mare’s serum gonadotropin and a COH protocol consisting of 10 IU/0.1 mL human chorionic gonadotropin injection after 48 h. This procedure was applied three and five times to group 2 and group 3, respectively. For the control groups, the same procedures were performed with 0.1 mL of 0.9% sodium chloride solution. At the end of the experiment, the ovarium tissues were placed in a 10% neutral formaldehyde solution for light microscopic examinations. In histological sections stained with hematoxylin and eosin, the number of ovarian follicles was determined using the physical dissector method. However, the expression of PTEN, FOXO3, and LH-R molecules was evaluated by immunohistochemical methods. As a result of our study, it was concluded that COH administration reduces the expression levels of PTEN and FOXO3 proteins and LH-R, which are among the essential components of the PIK3 intracellular signaling pathway and also increased the levels of hormones such as follicle-stimulating hormone, estradiol, and luteinizing hormone, which are over-reserve markers, and causes adverse effects on the histological structure, oocyte morphology, and number of ovaries. |
| first_indexed | 2024-12-10T03:42:59Z |
| format | Article |
| id | doaj.art-505e45b234f04327a57c8a960fae7c34 |
| institution | Directory Open Access Journal |
| issn | 2633-8386 |
| language | English |
| last_indexed | 2024-12-10T03:42:59Z |
| publishDate | 2022-09-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Reproduction and Fertility |
| spelling | doaj.art-505e45b234f04327a57c8a960fae7c342022-12-22T02:03:30ZengBioscientificaReproduction and Fertility2633-83862022-09-0133187197https://doi.org/10.1530/RAF-21-0075The effect of controlled ovarian hyperstimulation on ovarian reserve via PTEN pathwayPerihan Sezginer0Cigdem Elmas1Fatma Yıldız2Department of Medical Laboratory Techniques, Alanya Alaaddin Keykubat University, Health Services Vocational School, Alanya, TurkeyDepartment of Histology and Embryology, Gazi University, Faculty of Medicine, Ankara, TurkeyDepartment of Medical Laboratory Techniques, Alanya Alaaddin Keykubat University, Health Services Vocational School, Alanya, TurkeyThis study was carried out to investigate whether repeated controlled ovarian hyperstimulation (COH) affects ovarian reserve. For this reason, we aimed to show possible changes in the expression of PTEN and FOXO3, which are involved in preserving the over-reserve, after applying the COH protocol methods. For this purpose, 18 young Wistar albino female rats (8 weeks old) were randomly assigned as group 1 (control), group 2, and group 3 as 6 subjects in each group. Experimental groups were treated with 10 IU/0.1 mL pregnant mare’s serum gonadotropin and a COH protocol consisting of 10 IU/0.1 mL human chorionic gonadotropin injection after 48 h. This procedure was applied three and five times to group 2 and group 3, respectively. For the control groups, the same procedures were performed with 0.1 mL of 0.9% sodium chloride solution. At the end of the experiment, the ovarium tissues were placed in a 10% neutral formaldehyde solution for light microscopic examinations. In histological sections stained with hematoxylin and eosin, the number of ovarian follicles was determined using the physical dissector method. However, the expression of PTEN, FOXO3, and LH-R molecules was evaluated by immunohistochemical methods. As a result of our study, it was concluded that COH administration reduces the expression levels of PTEN and FOXO3 proteins and LH-R, which are among the essential components of the PIK3 intracellular signaling pathway and also increased the levels of hormones such as follicle-stimulating hormone, estradiol, and luteinizing hormone, which are over-reserve markers, and causes adverse effects on the histological structure, oocyte morphology, and number of ovaries.https://raf.bioscientifica.com/view/journals/raf/3/3/RAF-21-0075.xmlcontrolled ovarian hyperstimulationptenfoxo3lh-rovarian reserve |
| spellingShingle | Perihan Sezginer Cigdem Elmas Fatma Yıldız The effect of controlled ovarian hyperstimulation on ovarian reserve via PTEN pathway Reproduction and Fertility controlled ovarian hyperstimulation pten foxo3 lh-r ovarian reserve |
| title | The effect of controlled ovarian hyperstimulation on ovarian reserve via PTEN pathway |
| title_full | The effect of controlled ovarian hyperstimulation on ovarian reserve via PTEN pathway |
| title_fullStr | The effect of controlled ovarian hyperstimulation on ovarian reserve via PTEN pathway |
| title_full_unstemmed | The effect of controlled ovarian hyperstimulation on ovarian reserve via PTEN pathway |
| title_short | The effect of controlled ovarian hyperstimulation on ovarian reserve via PTEN pathway |
| title_sort | effect of controlled ovarian hyperstimulation on ovarian reserve via pten pathway |
| topic | controlled ovarian hyperstimulation pten foxo3 lh-r ovarian reserve |
| url | https://raf.bioscientifica.com/view/journals/raf/3/3/RAF-21-0075.xml |
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