Japanese Society of Allergology task force report on standardization of house dust mite allergen vaccines – Secondary publication
Background: In the 1990s, the Japanese Society of Allergology (JSA) standardized Japanese cedar pollen allergen vaccines. In the present study, the task force for house dust mite (HDM) allergen standardization of the Committee for Allergens and Immunotherapy of JSA reports the standardization of HDM...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-04-01
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| Series: | Allergology International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1323893015000672 |
| _version_ | 1811275129497321472 |
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| author | Toshiro Takai Yoshitaka Okamoto Kimihiro Okubo Makoto Nagata Masahiro Sakaguchi Yuma Fukutomi Akemi Saito Hiroshi Yasueda Keisuke Masuyama |
| author_facet | Toshiro Takai Yoshitaka Okamoto Kimihiro Okubo Makoto Nagata Masahiro Sakaguchi Yuma Fukutomi Akemi Saito Hiroshi Yasueda Keisuke Masuyama |
| author_sort | Toshiro Takai |
| collection | DOAJ |
| description | Background: In the 1990s, the Japanese Society of Allergology (JSA) standardized Japanese cedar pollen allergen vaccines. In the present study, the task force for house dust mite (HDM) allergen standardization of the Committee for Allergens and Immunotherapy of JSA reports the standardization of HDM allergen vaccines in Japan.
Methods: In vivo allergenic potency was determined by intradermal testing of 51 Japanese adults with positive serum specific IgE to HDM allergens. In vitro total IgE binding potency was analyzed by competition ELISA using a pooled serum, with sera obtained from 10 allergic patients. The amounts of HDM group 1 (Der 1) and group 2 major allergens in eight HDM allergen extracts were measured by sandwich ELISAs. Correlation between the in vitro total IgE binding potency and major allergen levels was analyzed.
Results: We selected a JSA reference HDM extract and determined its in vivo allergenic potency. The in vitro total IgE binding potency significantly correlated with Der 1 content, group 2 allergen content, and their combined amount, indicating that measurement of major allergen contents can be used as a surrogate in vitro assay.
Conclusions: The task force determined the in vivo allergenic potency (100,000 JAU/ml) and Der 1 content (38.5 μg/ml) of the JSA reference HDM extract, selected the measurement of Der 1 content as the surrogate in vitro assay, and decided that manufacturers can label a HDM allergen extract as having a titer of 100,000 JAU/ml if it contains 22.2–66.7 μg/ml of Der 1. |
| first_indexed | 2024-04-12T23:32:36Z |
| format | Article |
| id | doaj.art-50612728b2644d18b8376852f1ad2881 |
| institution | Directory Open Access Journal |
| issn | 1323-8930 |
| language | English |
| last_indexed | 2024-04-12T23:32:36Z |
| publishDate | 2015-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Allergology International |
| spelling | doaj.art-50612728b2644d18b8376852f1ad28812022-12-22T03:12:14ZengElsevierAllergology International1323-89302015-04-0164218118610.1016/j.alit.2015.01.005Japanese Society of Allergology task force report on standardization of house dust mite allergen vaccines – Secondary publicationToshiro Takai0Yoshitaka Okamoto1Kimihiro Okubo2Makoto Nagata3Masahiro Sakaguchi4Yuma Fukutomi5Akemi Saito6Hiroshi Yasueda7Keisuke Masuyama8Task Force for House Dust Mite Allergen Standardization of the Committee for Allergens and Immunotherapy of the Japanese Society of Allergology, Tokyo, JapanTask Force for House Dust Mite Allergen Standardization of the Committee for Allergens and Immunotherapy of the Japanese Society of Allergology, Tokyo, JapanTask Force for House Dust Mite Allergen Standardization of the Committee for Allergens and Immunotherapy of the Japanese Society of Allergology, Tokyo, JapanTask Force for House Dust Mite Allergen Standardization of the Committee for Allergens and Immunotherapy of the Japanese Society of Allergology, Tokyo, JapanTask Force for House Dust Mite Allergen Standardization of the Committee for Allergens and Immunotherapy of the Japanese Society of Allergology, Tokyo, JapanTask Force for House Dust Mite Allergen Standardization of the Committee for Allergens and Immunotherapy of the Japanese Society of Allergology, Tokyo, JapanClinical Research Center for Allergy and Rheumatology, Sagamihara National Hospital, Kanagawa, JapanTask Force for House Dust Mite Allergen Standardization of the Committee for Allergens and Immunotherapy of the Japanese Society of Allergology, Tokyo, JapanTask Force for House Dust Mite Allergen Standardization of the Committee for Allergens and Immunotherapy of the Japanese Society of Allergology, Tokyo, JapanBackground: In the 1990s, the Japanese Society of Allergology (JSA) standardized Japanese cedar pollen allergen vaccines. In the present study, the task force for house dust mite (HDM) allergen standardization of the Committee for Allergens and Immunotherapy of JSA reports the standardization of HDM allergen vaccines in Japan. Methods: In vivo allergenic potency was determined by intradermal testing of 51 Japanese adults with positive serum specific IgE to HDM allergens. In vitro total IgE binding potency was analyzed by competition ELISA using a pooled serum, with sera obtained from 10 allergic patients. The amounts of HDM group 1 (Der 1) and group 2 major allergens in eight HDM allergen extracts were measured by sandwich ELISAs. Correlation between the in vitro total IgE binding potency and major allergen levels was analyzed. Results: We selected a JSA reference HDM extract and determined its in vivo allergenic potency. The in vitro total IgE binding potency significantly correlated with Der 1 content, group 2 allergen content, and their combined amount, indicating that measurement of major allergen contents can be used as a surrogate in vitro assay. Conclusions: The task force determined the in vivo allergenic potency (100,000 JAU/ml) and Der 1 content (38.5 μg/ml) of the JSA reference HDM extract, selected the measurement of Der 1 content as the surrogate in vitro assay, and decided that manufacturers can label a HDM allergen extract as having a titer of 100,000 JAU/ml if it contains 22.2–66.7 μg/ml of Der 1.http://www.sciencedirect.com/science/article/pii/S1323893015000672House dust mite allergen standardizationIntradermal testingIn vivo allergenic potencyMajor allergen contentSurrogate in vitro assay |
| spellingShingle | Toshiro Takai Yoshitaka Okamoto Kimihiro Okubo Makoto Nagata Masahiro Sakaguchi Yuma Fukutomi Akemi Saito Hiroshi Yasueda Keisuke Masuyama Japanese Society of Allergology task force report on standardization of house dust mite allergen vaccines – Secondary publication Allergology International House dust mite allergen standardization Intradermal testing In vivo allergenic potency Major allergen content Surrogate in vitro assay |
| title | Japanese Society of Allergology task force report on standardization of house dust mite allergen vaccines – Secondary publication |
| title_full | Japanese Society of Allergology task force report on standardization of house dust mite allergen vaccines – Secondary publication |
| title_fullStr | Japanese Society of Allergology task force report on standardization of house dust mite allergen vaccines – Secondary publication |
| title_full_unstemmed | Japanese Society of Allergology task force report on standardization of house dust mite allergen vaccines – Secondary publication |
| title_short | Japanese Society of Allergology task force report on standardization of house dust mite allergen vaccines – Secondary publication |
| title_sort | japanese society of allergology task force report on standardization of house dust mite allergen vaccines secondary publication |
| topic | House dust mite allergen standardization Intradermal testing In vivo allergenic potency Major allergen content Surrogate in vitro assay |
| url | http://www.sciencedirect.com/science/article/pii/S1323893015000672 |
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