Porcine Epidemic Diarrhea Virus Replication in Human Intestinal Cells Reveals Potential Susceptibility to Cross-Species Infection
Various coronaviruses have emerged as a result of cross-species transmission among humans and domestic animals. Porcine epidemic diarrhea virus (PEDV; family Coronaviridae, genus Alphacoronavirus) causes acute diarrhea, vomiting, dehydration, and high mortality in neonatal piglets. Porcine small int...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/4/956 |
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| author | Zheng Niu Shujuan Zhang Shasha Xu Jing Wang Siying Wang Xia Hu Li Zhang Lixin Ren Jingyi Zhang Xiangyang Liu Yang Zhou Liu Yang Zhenhui Song |
| author_facet | Zheng Niu Shujuan Zhang Shasha Xu Jing Wang Siying Wang Xia Hu Li Zhang Lixin Ren Jingyi Zhang Xiangyang Liu Yang Zhou Liu Yang Zhenhui Song |
| author_sort | Zheng Niu |
| collection | DOAJ |
| description | Various coronaviruses have emerged as a result of cross-species transmission among humans and domestic animals. Porcine epidemic diarrhea virus (PEDV; family Coronaviridae, genus Alphacoronavirus) causes acute diarrhea, vomiting, dehydration, and high mortality in neonatal piglets. Porcine small intestinal epithelial cells (IPEC-J2 cells) can be used as target cells for PEDV infection. However, the origin of PEDV in pigs, the host range, and cross-species infection of PEDV remain unclear. To determine whether PEDV has the ability to infect human cells in vitro, human small intestinal epithelial cells (FHs 74 Int cells) were inoculated with PEDV LJX and PEDV CV777 strains. The results indicated that PEDV LJX, but not PEDV CV777, could infect FHs 74 Int cells. Furthermore, we observed M gene mRNA transcripts and N protein expression in infected FHs 74 Int cells. A one-step growth curve showed that the highest viral titer of PEDV occurred at 12 h post infection. Viral particles in vacuoles were observed in FHs 74 Int cells at 24 h post infection. The results proved that human small intestinal epithelial cells are susceptible to PEDV infection, suggesting the possibility of cross-species transmission of PEDV. |
| first_indexed | 2024-03-11T04:26:03Z |
| format | Article |
| id | doaj.art-50628e8c0ba74b82bbcf345f9276f36c |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T04:26:03Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-50628e8c0ba74b82bbcf345f9276f36c2023-11-17T21:46:10ZengMDPI AGViruses1999-49152023-04-0115495610.3390/v15040956Porcine Epidemic Diarrhea Virus Replication in Human Intestinal Cells Reveals Potential Susceptibility to Cross-Species InfectionZheng Niu0Shujuan Zhang1Shasha Xu2Jing Wang3Siying Wang4Xia Hu5Li Zhang6Lixin Ren7Jingyi Zhang8Xiangyang Liu9Yang Zhou10Liu Yang11Zhenhui Song12College of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaNational Center of Technology Innovation for Pigs, Chongqing 402460, ChinaCollege of Veterinary Medicine, Southwest University, Chongqing 402460, ChinaVarious coronaviruses have emerged as a result of cross-species transmission among humans and domestic animals. Porcine epidemic diarrhea virus (PEDV; family Coronaviridae, genus Alphacoronavirus) causes acute diarrhea, vomiting, dehydration, and high mortality in neonatal piglets. Porcine small intestinal epithelial cells (IPEC-J2 cells) can be used as target cells for PEDV infection. However, the origin of PEDV in pigs, the host range, and cross-species infection of PEDV remain unclear. To determine whether PEDV has the ability to infect human cells in vitro, human small intestinal epithelial cells (FHs 74 Int cells) were inoculated with PEDV LJX and PEDV CV777 strains. The results indicated that PEDV LJX, but not PEDV CV777, could infect FHs 74 Int cells. Furthermore, we observed M gene mRNA transcripts and N protein expression in infected FHs 74 Int cells. A one-step growth curve showed that the highest viral titer of PEDV occurred at 12 h post infection. Viral particles in vacuoles were observed in FHs 74 Int cells at 24 h post infection. The results proved that human small intestinal epithelial cells are susceptible to PEDV infection, suggesting the possibility of cross-species transmission of PEDV.https://www.mdpi.com/1999-4915/15/4/956PEDVhuman small intestinal epithelial cellscross-species transmission |
| spellingShingle | Zheng Niu Shujuan Zhang Shasha Xu Jing Wang Siying Wang Xia Hu Li Zhang Lixin Ren Jingyi Zhang Xiangyang Liu Yang Zhou Liu Yang Zhenhui Song Porcine Epidemic Diarrhea Virus Replication in Human Intestinal Cells Reveals Potential Susceptibility to Cross-Species Infection Viruses PEDV human small intestinal epithelial cells cross-species transmission |
| title | Porcine Epidemic Diarrhea Virus Replication in Human Intestinal Cells Reveals Potential Susceptibility to Cross-Species Infection |
| title_full | Porcine Epidemic Diarrhea Virus Replication in Human Intestinal Cells Reveals Potential Susceptibility to Cross-Species Infection |
| title_fullStr | Porcine Epidemic Diarrhea Virus Replication in Human Intestinal Cells Reveals Potential Susceptibility to Cross-Species Infection |
| title_full_unstemmed | Porcine Epidemic Diarrhea Virus Replication in Human Intestinal Cells Reveals Potential Susceptibility to Cross-Species Infection |
| title_short | Porcine Epidemic Diarrhea Virus Replication in Human Intestinal Cells Reveals Potential Susceptibility to Cross-Species Infection |
| title_sort | porcine epidemic diarrhea virus replication in human intestinal cells reveals potential susceptibility to cross species infection |
| topic | PEDV human small intestinal epithelial cells cross-species transmission |
| url | https://www.mdpi.com/1999-4915/15/4/956 |
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