Effect of temperature and humidity on dynamics and transmission of Pseudomonas amygdali pv. lachrymans aerosols
Cucumber angular leaf spot (ALS) disease, caused by Pseudomonas amygdali pv. lachrymans (Pal), is an emerging disease with a high incidence that causes severe damage to cucumber worldwide. Bacterial aerosols play a crucial role in the epidemiology of greenhouse ALS disease. However, little is known...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1087496/full |
| _version_ | 1811173012784807936 |
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| author | Ali Chai Lifang Yuan Lifang Yuan Xin Li Lei Li Yanxia Shi Xuewen Xie Baoju Li |
| author_facet | Ali Chai Lifang Yuan Lifang Yuan Xin Li Lei Li Yanxia Shi Xuewen Xie Baoju Li |
| author_sort | Ali Chai |
| collection | DOAJ |
| description | Cucumber angular leaf spot (ALS) disease, caused by Pseudomonas amygdali pv. lachrymans (Pal), is an emerging disease with a high incidence that causes severe damage to cucumber worldwide. Bacterial aerosols play a crucial role in the epidemiology of greenhouse ALS disease. However, little is known about the influence of temperature and relative humidity (RH) on the dynamics of Pal in aerosols. A study was conducted to investigate the relationships between the concentration of Pal aerosols and their dependence on temperature and RH in aerosol chambers and greenhouses. The results demonstrated that temperature and RH are both significant factors influencing the release amount, survival time and infectivity of Pal in aerosols, while RH has a greater influence on particle size than temperature across the range of conditions tested. The release amount and survival time of Pal in aerosols under high RH (95%) and low temperature (≤ 25°C) conditions were significantly higher than those under low RH (35%) and high temperature (35°C) conditions. The highest release amount of Pal aerosol (96 CFU/m3) and highest survival rate (98.41%) were found at 18°C and 95% RH, while the highest disease index (DI = 60.9) caused by Pal aerosol was found at 25°C and 95% RH. In addition, Pal aerosols presented a larger diameter (4.7->7.0 μm) under high RH (95% RH) than under dry conditions (≤ 65% RH). These findings will play a crucial role in elucidating the influence of environmental parameters on the dynamics and transmission of Pal in aerosols. Based on our findings, preliminary recommendations for controlling airborne Pal spread involve controlling air temperature and RH, which will contribute to the effective alleviation and control of cucumber ALS disease. |
| first_indexed | 2024-04-10T17:40:58Z |
| format | Article |
| id | doaj.art-5064659679c544ce8ea7be7298f09bef |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T17:40:58Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-5064659679c544ce8ea7be7298f09bef2023-02-03T13:46:06ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-02-011410.3389/fpls.2023.10874961087496Effect of temperature and humidity on dynamics and transmission of Pseudomonas amygdali pv. lachrymans aerosolsAli Chai0Lifang Yuan1Lifang Yuan2Xin Li3Lei Li4Yanxia Shi5Xuewen Xie6Baoju Li7Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaShandong Academy of Agricultural Sciences, Shandong Academy of Grape, Jinan, Shandong, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, ChinaCucumber angular leaf spot (ALS) disease, caused by Pseudomonas amygdali pv. lachrymans (Pal), is an emerging disease with a high incidence that causes severe damage to cucumber worldwide. Bacterial aerosols play a crucial role in the epidemiology of greenhouse ALS disease. However, little is known about the influence of temperature and relative humidity (RH) on the dynamics of Pal in aerosols. A study was conducted to investigate the relationships between the concentration of Pal aerosols and their dependence on temperature and RH in aerosol chambers and greenhouses. The results demonstrated that temperature and RH are both significant factors influencing the release amount, survival time and infectivity of Pal in aerosols, while RH has a greater influence on particle size than temperature across the range of conditions tested. The release amount and survival time of Pal in aerosols under high RH (95%) and low temperature (≤ 25°C) conditions were significantly higher than those under low RH (35%) and high temperature (35°C) conditions. The highest release amount of Pal aerosol (96 CFU/m3) and highest survival rate (98.41%) were found at 18°C and 95% RH, while the highest disease index (DI = 60.9) caused by Pal aerosol was found at 25°C and 95% RH. In addition, Pal aerosols presented a larger diameter (4.7->7.0 μm) under high RH (95% RH) than under dry conditions (≤ 65% RH). These findings will play a crucial role in elucidating the influence of environmental parameters on the dynamics and transmission of Pal in aerosols. Based on our findings, preliminary recommendations for controlling airborne Pal spread involve controlling air temperature and RH, which will contribute to the effective alleviation and control of cucumber ALS disease.https://www.frontiersin.org/articles/10.3389/fpls.2023.1087496/fulltemperaturerelative humiditybioaerosolPseudomonas amygdali pv. lachrymanscucumber angular leaf spot disease |
| spellingShingle | Ali Chai Lifang Yuan Lifang Yuan Xin Li Lei Li Yanxia Shi Xuewen Xie Baoju Li Effect of temperature and humidity on dynamics and transmission of Pseudomonas amygdali pv. lachrymans aerosols Frontiers in Plant Science temperature relative humidity bioaerosol Pseudomonas amygdali pv. lachrymans cucumber angular leaf spot disease |
| title | Effect of temperature and humidity on dynamics and transmission of Pseudomonas amygdali pv. lachrymans aerosols |
| title_full | Effect of temperature and humidity on dynamics and transmission of Pseudomonas amygdali pv. lachrymans aerosols |
| title_fullStr | Effect of temperature and humidity on dynamics and transmission of Pseudomonas amygdali pv. lachrymans aerosols |
| title_full_unstemmed | Effect of temperature and humidity on dynamics and transmission of Pseudomonas amygdali pv. lachrymans aerosols |
| title_short | Effect of temperature and humidity on dynamics and transmission of Pseudomonas amygdali pv. lachrymans aerosols |
| title_sort | effect of temperature and humidity on dynamics and transmission of pseudomonas amygdali pv lachrymans aerosols |
| topic | temperature relative humidity bioaerosol Pseudomonas amygdali pv. lachrymans cucumber angular leaf spot disease |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1087496/full |
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