Histological criteria for selecting patients who need clonality test for non-gastric MALT lymphoma diagnosis
Abstract The histological diagnosis of extranodal marginal zone lymphoma of the mucosa-associated lymphoid tissue (MALT lymphoma) is difficult for pathologists. Recently, digital pathology systems have been widely used to provide tools that can objectively measure lesions on slides. In this study, w...
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| Format: | Article |
| Language: | English |
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BMC
2024-03-01
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| Series: | Diagnostic Pathology |
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| Online Access: | https://doi.org/10.1186/s13000-024-01471-8 |
| _version_ | 1827321546134257664 |
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| author | Dajeong Park Junhun Cho |
| author_facet | Dajeong Park Junhun Cho |
| author_sort | Dajeong Park |
| collection | DOAJ |
| description | Abstract The histological diagnosis of extranodal marginal zone lymphoma of the mucosa-associated lymphoid tissue (MALT lymphoma) is difficult for pathologists. Recently, digital pathology systems have been widely used to provide tools that can objectively measure lesions on slides. In this study, we measured the extent of marginal zone expansion in suspected MALT lymphoma cases and compared the results with those of a molecular clonality test. In total, 115 patients who underwent an IGH gene rearrangement test for suspected MALT lymphoma were included in this study. All cases were histologically classified into three patterns; “small lymphoid aggregates with no germinal center (Pattern 1),” “lymphoid follicles with germinal center (Pattern 2)” and " fused marginal zone or diffuse small lymphocytic proliferation (Pattern 3).” The proportions of monoclonality in Pattern 1, 2, and 3 were 25.0%, 55.0%, and 97.9%, respectively. The ratios of marginal zone thickness to germinal center diameter and entire lymphoid follicle area to germinal center area were measured in Pattern 2 cases using a digital pathology system. Combining the width cutoff of 1.5 and the areal cutoff of 3.5, the sensitivity, specificity, positive predictive value, and negative predictive value for MALT lymphoma were 96.97%, 70.37%, 80.00%, and 95.00%, respectively. In conclusion, through objective measurement of the marginal zone, suspected cases of MALT lymphoma requiring a molecular clonality test can be effectively selected. |
| first_indexed | 2024-04-25T01:09:14Z |
| format | Article |
| id | doaj.art-50654ac7f8324fd1b9af6098b07eb84d |
| institution | Directory Open Access Journal |
| issn | 1746-1596 |
| language | English |
| last_indexed | 2024-04-25T01:09:14Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Diagnostic Pathology |
| spelling | doaj.art-50654ac7f8324fd1b9af6098b07eb84d2024-03-10T12:04:44ZengBMCDiagnostic Pathology1746-15962024-03-011911710.1186/s13000-024-01471-8Histological criteria for selecting patients who need clonality test for non-gastric MALT lymphoma diagnosisDajeong Park0Junhun Cho1Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of MedicineDepartment of Pathology, Samsung Medical Center, Sungkyunkwan University School of MedicineAbstract The histological diagnosis of extranodal marginal zone lymphoma of the mucosa-associated lymphoid tissue (MALT lymphoma) is difficult for pathologists. Recently, digital pathology systems have been widely used to provide tools that can objectively measure lesions on slides. In this study, we measured the extent of marginal zone expansion in suspected MALT lymphoma cases and compared the results with those of a molecular clonality test. In total, 115 patients who underwent an IGH gene rearrangement test for suspected MALT lymphoma were included in this study. All cases were histologically classified into three patterns; “small lymphoid aggregates with no germinal center (Pattern 1),” “lymphoid follicles with germinal center (Pattern 2)” and " fused marginal zone or diffuse small lymphocytic proliferation (Pattern 3).” The proportions of monoclonality in Pattern 1, 2, and 3 were 25.0%, 55.0%, and 97.9%, respectively. The ratios of marginal zone thickness to germinal center diameter and entire lymphoid follicle area to germinal center area were measured in Pattern 2 cases using a digital pathology system. Combining the width cutoff of 1.5 and the areal cutoff of 3.5, the sensitivity, specificity, positive predictive value, and negative predictive value for MALT lymphoma were 96.97%, 70.37%, 80.00%, and 95.00%, respectively. In conclusion, through objective measurement of the marginal zone, suspected cases of MALT lymphoma requiring a molecular clonality test can be effectively selected.https://doi.org/10.1186/s13000-024-01471-8Extranodal marginal zone lymphoma of MALTMALT lymphomaMarginal zone expansionDigital pathology |
| spellingShingle | Dajeong Park Junhun Cho Histological criteria for selecting patients who need clonality test for non-gastric MALT lymphoma diagnosis Diagnostic Pathology Extranodal marginal zone lymphoma of MALT MALT lymphoma Marginal zone expansion Digital pathology |
| title | Histological criteria for selecting patients who need clonality test for non-gastric MALT lymphoma diagnosis |
| title_full | Histological criteria for selecting patients who need clonality test for non-gastric MALT lymphoma diagnosis |
| title_fullStr | Histological criteria for selecting patients who need clonality test for non-gastric MALT lymphoma diagnosis |
| title_full_unstemmed | Histological criteria for selecting patients who need clonality test for non-gastric MALT lymphoma diagnosis |
| title_short | Histological criteria for selecting patients who need clonality test for non-gastric MALT lymphoma diagnosis |
| title_sort | histological criteria for selecting patients who need clonality test for non gastric malt lymphoma diagnosis |
| topic | Extranodal marginal zone lymphoma of MALT MALT lymphoma Marginal zone expansion Digital pathology |
| url | https://doi.org/10.1186/s13000-024-01471-8 |
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