Revealing alteration of membrane structures during ischema using impedance spectroscopy
Alterations of membrane structure and function are essential characteristics of cells undergoing ischemia. Noninvasive monitoring of tissue alterations during ischemia and the estimation of the reversibility domain (corresponding to organ capability to fully recover its functions after shifting back...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2002-11-01
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| Series: | Songklanakarin Journal of Science and Technology (SJST) |
| Subjects: | |
| Online Access: | http://www.sjst.psu.ac.th/journal/24_Suppl_membrane/02ischema.pdf |
| _version_ | 1818553757655367680 |
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| author | Mihaela Gheorghiu Eberhard Gersing |
| author_facet | Mihaela Gheorghiu Eberhard Gersing |
| author_sort | Mihaela Gheorghiu |
| collection | DOAJ |
| description | Alterations of membrane structure and function are essential characteristics of cells undergoing ischemia. Noninvasive monitoring of tissue alterations during ischemia and the estimation of the reversibility domain (corresponding to organ capability to fully recover its functions after shifting back to normal blood perfusion) are important for biomedical applications allowing better time management during surgical interventions, especially in organ transplantation. Due to it’s capability to reveal inhomogeneities, as well as it’s noninvasive character, impedance spectroscopy was used for continuous monitoring of the progression of excised tissue samples during ischemia. We have developed a fast, noninvasive, automated method for quantitative analysis of impedance spectra of tissue samples, capable of revealing, through characteristic parameters (dispersion amplitudes, time constants and distribution parameters) membrane based microscopic processes like the closure ofgap-junctions (a characteristic of the early alterations of ischemic tissues in the reversibility phase). Microscopic and equivalent circuit modeling was used to probe the effect of closure of cell connections and of changes in electrical properties of cell constituents on impedance spectra. We have developed a normalizing procedure emphasizing the pattern of ischemic alterations and enabling the comparison of different data sets. |
| first_indexed | 2024-12-12T09:30:00Z |
| format | Article |
| id | doaj.art-5065506bbccd46fdbb93942c8e96f463 |
| institution | Directory Open Access Journal |
| issn | 0125-3395 |
| language | English |
| last_indexed | 2024-12-12T09:30:00Z |
| publishDate | 2002-11-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Songklanakarin Journal of Science and Technology (SJST) |
| spelling | doaj.art-5065506bbccd46fdbb93942c8e96f4632022-12-22T00:28:53ZengPrince of Songkla UniversitySongklanakarin Journal of Science and Technology (SJST)0125-33952002-11-0124Suppl.777784Revealing alteration of membrane structures during ischema using impedance spectroscopyMihaela GheorghiuEberhard GersingAlterations of membrane structure and function are essential characteristics of cells undergoing ischemia. Noninvasive monitoring of tissue alterations during ischemia and the estimation of the reversibility domain (corresponding to organ capability to fully recover its functions after shifting back to normal blood perfusion) are important for biomedical applications allowing better time management during surgical interventions, especially in organ transplantation. Due to it’s capability to reveal inhomogeneities, as well as it’s noninvasive character, impedance spectroscopy was used for continuous monitoring of the progression of excised tissue samples during ischemia. We have developed a fast, noninvasive, automated method for quantitative analysis of impedance spectra of tissue samples, capable of revealing, through characteristic parameters (dispersion amplitudes, time constants and distribution parameters) membrane based microscopic processes like the closure ofgap-junctions (a characteristic of the early alterations of ischemic tissues in the reversibility phase). Microscopic and equivalent circuit modeling was used to probe the effect of closure of cell connections and of changes in electrical properties of cell constituents on impedance spectra. We have developed a normalizing procedure emphasizing the pattern of ischemic alterations and enabling the comparison of different data sets.http://www.sjst.psu.ac.th/journal/24_Suppl_membrane/02ischema.pdfalteration of membrane structureischemaimpedance spectroscopy |
| spellingShingle | Mihaela Gheorghiu Eberhard Gersing Revealing alteration of membrane structures during ischema using impedance spectroscopy Songklanakarin Journal of Science and Technology (SJST) alteration of membrane structure ischema impedance spectroscopy |
| title | Revealing alteration of membrane structures during ischema using impedance spectroscopy |
| title_full | Revealing alteration of membrane structures during ischema using impedance spectroscopy |
| title_fullStr | Revealing alteration of membrane structures during ischema using impedance spectroscopy |
| title_full_unstemmed | Revealing alteration of membrane structures during ischema using impedance spectroscopy |
| title_short | Revealing alteration of membrane structures during ischema using impedance spectroscopy |
| title_sort | revealing alteration of membrane structures during ischema using impedance spectroscopy |
| topic | alteration of membrane structure ischema impedance spectroscopy |
| url | http://www.sjst.psu.ac.th/journal/24_Suppl_membrane/02ischema.pdf |
| work_keys_str_mv | AT mihaelagheorghiu revealingalterationofmembranestructuresduringischemausingimpedancespectroscopy AT eberhardgersing revealingalterationofmembranestructuresduringischemausingimpedancespectroscopy |