Genes and genetic mechanisms contributing to fall armyworm resistance in maize
Abstract Maize (Zea mays L.) is a crop of major economic and food security importance globally. The fall armyworm (FAW), Spodoptera frugiperda, can devastate entire maize crops, especially in countries or markets that do not allow the use of transgenic crops. Host‐plant insect resistance is an econo...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-06-01
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| Series: | The Plant Genome |
| Online Access: | https://doi.org/10.1002/tpg2.20311 |
| _version_ | 1797800498422611968 |
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| author | Marilyn L. Warburton Sandra W. Woolfolk J. Spencer Smith Leigh K. Hawkins Lina Castano‐Duque Matthew D. Lebar W. Paul Williams |
| author_facet | Marilyn L. Warburton Sandra W. Woolfolk J. Spencer Smith Leigh K. Hawkins Lina Castano‐Duque Matthew D. Lebar W. Paul Williams |
| author_sort | Marilyn L. Warburton |
| collection | DOAJ |
| description | Abstract Maize (Zea mays L.) is a crop of major economic and food security importance globally. The fall armyworm (FAW), Spodoptera frugiperda, can devastate entire maize crops, especially in countries or markets that do not allow the use of transgenic crops. Host‐plant insect resistance is an economical and environmentally benign way to control FAW, and this study sought to identify maize lines, genes, and pathways that contribute to resistance to FAW. Of the 289 maize lines phenotyped for FAW damage in artificially infested, replicated field trials over 3 years, 31 were identified with good levels of resistance that could donate FAW resistance into elite but susceptible hybrid parents. The 289 lines were genotyped by sequencing to provide single nucleotide polymorphism (SNP) markers for a genome‐wide association study (GWAS), followed by a metabolic pathway analysis using the Pathway Association Study Tool (PAST). GWAS identified 15 SNPs linked to 7 genes, and PAST identified multiple pathways, associated with FAW damage. Top pathways, and thus useful resistance mechanisms for further study, include hormone signaling pathways and the biosynthesis of carotenoids (particularly zeaxanthin), chlorophyll compounds, cuticular wax, known antibiosis agents, and 1,4‐dihydroxy‐2‐naphthoate. Targeted metabolite analysis confirmed that maize genotypes with lower levels of FAW damage tend to have higher levels of chlorophyll a than genotypes with high FAW damage, which tend to have lower levels of pheophytin, lutein, chlorophyll b and β‐carotene. The list of resistant genotypes, and the results from the genetic, pathway, and metabolic study, can all contribute to efficient creation of FAW resistant cultivars. |
| first_indexed | 2024-03-13T04:36:11Z |
| format | Article |
| id | doaj.art-5065de707487438c8a3116e2ca8087a9 |
| institution | Directory Open Access Journal |
| issn | 1940-3372 |
| language | English |
| last_indexed | 2024-03-13T04:36:11Z |
| publishDate | 2023-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Plant Genome |
| spelling | doaj.art-5065de707487438c8a3116e2ca8087a92023-06-19T06:48:03ZengWileyThe Plant Genome1940-33722023-06-01162n/an/a10.1002/tpg2.20311Genes and genetic mechanisms contributing to fall armyworm resistance in maizeMarilyn L. Warburton0Sandra W. Woolfolk1J. Spencer Smith2Leigh K. Hawkins3Lina Castano‐Duque4Matthew D. Lebar5W. Paul Williams6USDA ARS Plant Germplasm Introduction and Testing Research Unit Pullman WA USAUSDA ARS Corn Host Plant Resistance Research Unit Mississippi State MS USAUSDA ARS Corn Host Plant Resistance Research Unit Mississippi State MS USAUSDA ARS Corn Host Plant Resistance Research Unit Mississippi State MS USAUSDA ARS Food and Feed Safety Research Unit New Orleans LA USAUSDA ARS Food and Feed Safety Research Unit New Orleans LA USAUSDA ARS Corn Host Plant Resistance Research Unit Mississippi State MS USAAbstract Maize (Zea mays L.) is a crop of major economic and food security importance globally. The fall armyworm (FAW), Spodoptera frugiperda, can devastate entire maize crops, especially in countries or markets that do not allow the use of transgenic crops. Host‐plant insect resistance is an economical and environmentally benign way to control FAW, and this study sought to identify maize lines, genes, and pathways that contribute to resistance to FAW. Of the 289 maize lines phenotyped for FAW damage in artificially infested, replicated field trials over 3 years, 31 were identified with good levels of resistance that could donate FAW resistance into elite but susceptible hybrid parents. The 289 lines were genotyped by sequencing to provide single nucleotide polymorphism (SNP) markers for a genome‐wide association study (GWAS), followed by a metabolic pathway analysis using the Pathway Association Study Tool (PAST). GWAS identified 15 SNPs linked to 7 genes, and PAST identified multiple pathways, associated with FAW damage. Top pathways, and thus useful resistance mechanisms for further study, include hormone signaling pathways and the biosynthesis of carotenoids (particularly zeaxanthin), chlorophyll compounds, cuticular wax, known antibiosis agents, and 1,4‐dihydroxy‐2‐naphthoate. Targeted metabolite analysis confirmed that maize genotypes with lower levels of FAW damage tend to have higher levels of chlorophyll a than genotypes with high FAW damage, which tend to have lower levels of pheophytin, lutein, chlorophyll b and β‐carotene. The list of resistant genotypes, and the results from the genetic, pathway, and metabolic study, can all contribute to efficient creation of FAW resistant cultivars.https://doi.org/10.1002/tpg2.20311 |
| spellingShingle | Marilyn L. Warburton Sandra W. Woolfolk J. Spencer Smith Leigh K. Hawkins Lina Castano‐Duque Matthew D. Lebar W. Paul Williams Genes and genetic mechanisms contributing to fall armyworm resistance in maize The Plant Genome |
| title | Genes and genetic mechanisms contributing to fall armyworm resistance in maize |
| title_full | Genes and genetic mechanisms contributing to fall armyworm resistance in maize |
| title_fullStr | Genes and genetic mechanisms contributing to fall armyworm resistance in maize |
| title_full_unstemmed | Genes and genetic mechanisms contributing to fall armyworm resistance in maize |
| title_short | Genes and genetic mechanisms contributing to fall armyworm resistance in maize |
| title_sort | genes and genetic mechanisms contributing to fall armyworm resistance in maize |
| url | https://doi.org/10.1002/tpg2.20311 |
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