Genome-Wide Analysis of the <i>IQM</i> Gene Family in Rice (<i>Oryza sativa</i> L.)
Members of the <i>IQM</i> (IQ-Motif Containing) gene family are involved in plant growth and developmental processes, biotic and abiotic stress response. To systematically analyze the <i>IQM</i> gene family and their expression profiles under diverse biotic and abiotic stress...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/9/1949 |
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| author | Tian Fan Tianxiao Lv Chuping Xie Yuping Zhou Changen Tian |
| author_facet | Tian Fan Tianxiao Lv Chuping Xie Yuping Zhou Changen Tian |
| author_sort | Tian Fan |
| collection | DOAJ |
| description | Members of the <i>IQM</i> (IQ-Motif Containing) gene family are involved in plant growth and developmental processes, biotic and abiotic stress response. To systematically analyze the <i>IQM</i> gene family and their expression profiles under diverse biotic and abiotic stresses, we identified 8 <i>IQM</i> genes in the rice genome. In the current study, the whole genome identification and characterization of <i>OsIQMs</i>, including the gene and protein structure, genome localization, phylogenetic relationship, gene expression and yeast two-hybrid were performed. Eight <i>IQM</i> genes were classified into three subfamilies (I–III) according to the phylogenetic analysis. Gene structure and protein motif analyses showed that these <i>IQM</i> genes are relatively conserved within each subfamily of rice. The 8 <i>OsIQM</i> genes are distributed on seven out of the twelve chromosomes, with three <i>IQM</i> gene pairs involved in segmental duplication events. The evolutionary patterns analysis revealed that the <i>IQM</i> genes underwent a large-scale event within the last 20 to 9 million years. In addition, quantitative real-time PCR analysis of eight <i>OsIQMs</i> genes displayed different expression patterns at different developmental stages and in different tissues as well as showed that most <i>IQM</i> genes were responsive to PEG, NaCl, jasmonic acid (JA), abscisic acid (ABA) treatment, suggesting their crucial roles in biotic, and abiotic stress response. Additionally, a yeast two-hybrid assay showed that OsIQMs can interact with OsCaMs, and the IQ motif of OsIQMs is required for OsIQMs to combine with OsCaMs. Our results will be valuable to further characterize the important biological functions of rice <i>IQM</i> genes. |
| first_indexed | 2024-03-10T07:17:19Z |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T07:17:19Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-50684d75bbc84a17965a945a25a116222023-11-22T14:54:13ZengMDPI AGPlants2223-77472021-09-01109194910.3390/plants10091949Genome-Wide Analysis of the <i>IQM</i> Gene Family in Rice (<i>Oryza sativa</i> L.)Tian Fan0Tianxiao Lv1Chuping Xie2Yuping Zhou3Changen Tian4Guangzhou Key Laboratory for Functional Study on Plant Stress-Resistant Genes, School of Life Sciences, Guangzhou University, Guangzhou 510006, ChinaGuangzhou Key Laboratory for Functional Study on Plant Stress-Resistant Genes, School of Life Sciences, Guangzhou University, Guangzhou 510006, ChinaGuangzhou Key Laboratory for Functional Study on Plant Stress-Resistant Genes, School of Life Sciences, Guangzhou University, Guangzhou 510006, ChinaGuangzhou Key Laboratory for Functional Study on Plant Stress-Resistant Genes, School of Life Sciences, Guangzhou University, Guangzhou 510006, ChinaGuangzhou Key Laboratory for Functional Study on Plant Stress-Resistant Genes, School of Life Sciences, Guangzhou University, Guangzhou 510006, ChinaMembers of the <i>IQM</i> (IQ-Motif Containing) gene family are involved in plant growth and developmental processes, biotic and abiotic stress response. To systematically analyze the <i>IQM</i> gene family and their expression profiles under diverse biotic and abiotic stresses, we identified 8 <i>IQM</i> genes in the rice genome. In the current study, the whole genome identification and characterization of <i>OsIQMs</i>, including the gene and protein structure, genome localization, phylogenetic relationship, gene expression and yeast two-hybrid were performed. Eight <i>IQM</i> genes were classified into three subfamilies (I–III) according to the phylogenetic analysis. Gene structure and protein motif analyses showed that these <i>IQM</i> genes are relatively conserved within each subfamily of rice. The 8 <i>OsIQM</i> genes are distributed on seven out of the twelve chromosomes, with three <i>IQM</i> gene pairs involved in segmental duplication events. The evolutionary patterns analysis revealed that the <i>IQM</i> genes underwent a large-scale event within the last 20 to 9 million years. In addition, quantitative real-time PCR analysis of eight <i>OsIQMs</i> genes displayed different expression patterns at different developmental stages and in different tissues as well as showed that most <i>IQM</i> genes were responsive to PEG, NaCl, jasmonic acid (JA), abscisic acid (ABA) treatment, suggesting their crucial roles in biotic, and abiotic stress response. Additionally, a yeast two-hybrid assay showed that OsIQMs can interact with OsCaMs, and the IQ motif of OsIQMs is required for OsIQMs to combine with OsCaMs. Our results will be valuable to further characterize the important biological functions of rice <i>IQM</i> genes.https://www.mdpi.com/2223-7747/10/9/1949rice<i>IQM</i> genesexpression patternIQ motif |
| spellingShingle | Tian Fan Tianxiao Lv Chuping Xie Yuping Zhou Changen Tian Genome-Wide Analysis of the <i>IQM</i> Gene Family in Rice (<i>Oryza sativa</i> L.) Plants rice <i>IQM</i> genes expression pattern IQ motif |
| title | Genome-Wide Analysis of the <i>IQM</i> Gene Family in Rice (<i>Oryza sativa</i> L.) |
| title_full | Genome-Wide Analysis of the <i>IQM</i> Gene Family in Rice (<i>Oryza sativa</i> L.) |
| title_fullStr | Genome-Wide Analysis of the <i>IQM</i> Gene Family in Rice (<i>Oryza sativa</i> L.) |
| title_full_unstemmed | Genome-Wide Analysis of the <i>IQM</i> Gene Family in Rice (<i>Oryza sativa</i> L.) |
| title_short | Genome-Wide Analysis of the <i>IQM</i> Gene Family in Rice (<i>Oryza sativa</i> L.) |
| title_sort | genome wide analysis of the i iqm i gene family in rice i oryza sativa i l |
| topic | rice <i>IQM</i> genes expression pattern IQ motif |
| url | https://www.mdpi.com/2223-7747/10/9/1949 |
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