Selective Recovery of Polyphenols from Discarded Blueberries (<i>Vaccinium corymbosum</i> L.) Using Hot Pressurized Liquid Extraction Combined with Isopropanol as an Environmentally Friendly Solvent
The use of water–ethanol mixtures in hot pressurized liquid extraction (HPLE) to recover phenolic compounds from agro-industrial waste has been successfully investigated. However, the unresolved challenge of reducing solvent costs associated with the process hinders the scaling of this eco-friendly...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/19/3694 |
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| author | Nils Leander Huamán-Castilla Cecilia Copa-Chipana Luis Omar Mamani-Apaza Olivia Magaly Luque-Vilca Clara Nely Campos-Quiróz Franz Zirena-Vilca María Salomé Mariotti-Celis |
| author_facet | Nils Leander Huamán-Castilla Cecilia Copa-Chipana Luis Omar Mamani-Apaza Olivia Magaly Luque-Vilca Clara Nely Campos-Quiróz Franz Zirena-Vilca María Salomé Mariotti-Celis |
| author_sort | Nils Leander Huamán-Castilla |
| collection | DOAJ |
| description | The use of water–ethanol mixtures in hot pressurized liquid extraction (HPLE) to recover phenolic compounds from agro-industrial waste has been successfully investigated. However, the unresolved challenge of reducing solvent costs associated with the process hinders the scaling of this eco-friendly technology. This study evaluated the use of isopropanol as an alternative, lower-cost solvent for recovering polyphenols from discarded blueberries through the HPLE process. HPLE was carried out using water–isopropanol mixtures (0, 15 and 30%) at 70, 100, and 130 °C. The total polyphenol content (TPC), antioxidant capacity (DPPH and ORAC), glucose and fructose contents, and polyphenol profile of the extracts were determined. HPLE extracts obtained using high isopropanol concentrations (30%) and high temperatures (130 °C) presented the highest TPC (13.57 mg GAE/gdw) and antioxidant capacity (IC50: 9.97 mg/mL, ORAC: 246.47 µmol ET/gdw). Moreover, the use of 30% water–isopropanol resulted in higher yields of polyphenols and removal of reducing sugars compared to atmospheric extraction with water–acetone (60%). The polyphenolic profiles of the extracts showed that flavanols and phenolic acids were more soluble at high concentrations of isopropanol (30%). Contrarily, flavonols and stilbenes were better recovered with 15% isopropanol and pure water. Therefore, isopropanol could be a promising solvent for the selective recovery of different bioactive compounds from discarded blueberries and other agro-industrial residues. |
| first_indexed | 2024-03-10T21:44:35Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T21:44:35Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-5068b9909d8b437a93c11655ec80accc2023-11-19T14:24:25ZengMDPI AGFoods2304-81582023-10-011219369410.3390/foods12193694Selective Recovery of Polyphenols from Discarded Blueberries (<i>Vaccinium corymbosum</i> L.) Using Hot Pressurized Liquid Extraction Combined with Isopropanol as an Environmentally Friendly SolventNils Leander Huamán-Castilla0Cecilia Copa-Chipana1Luis Omar Mamani-Apaza2Olivia Magaly Luque-Vilca3Clara Nely Campos-Quiróz4Franz Zirena-Vilca5María Salomé Mariotti-Celis6Escuela de Ingeniería Agroindustrial, Universidad Nacional de Moquegua, Prolongación Calle Ancash s/n, Moquegua 18001, PeruEscuela de Ingeniería Agroindustrial, Universidad Nacional de Moquegua, Prolongación Calle Ancash s/n, Moquegua 18001, PeruEscuela de Ingeniería Agroindustrial, Universidad Nacional de Moquegua, Prolongación Calle Ancash s/n, Moquegua 18001, PeruEscuela de Ingeniería en Industrias Alimentarias, Universidad Nacional de Juliaca, Av. Nueva Zelandia 631, Juliaca 21101, PeruLaboratorio de Contaminantes Orgánicos y Ambiente, Instituto de Investigación para el Desarrollo del Perú (IINDEP), Universidad Nacional de Moquegua, Moquegua 18001, PeruEscuela de Ingeniería Agroindustrial, Universidad Nacional de Moquegua, Prolongación Calle Ancash s/n, Moquegua 18001, PeruEscuela de Nutrición y Dietética, Facultad de Medicina, Universidad Finis Terrae, Santiago 7501015, ChileThe use of water–ethanol mixtures in hot pressurized liquid extraction (HPLE) to recover phenolic compounds from agro-industrial waste has been successfully investigated. However, the unresolved challenge of reducing solvent costs associated with the process hinders the scaling of this eco-friendly technology. This study evaluated the use of isopropanol as an alternative, lower-cost solvent for recovering polyphenols from discarded blueberries through the HPLE process. HPLE was carried out using water–isopropanol mixtures (0, 15 and 30%) at 70, 100, and 130 °C. The total polyphenol content (TPC), antioxidant capacity (DPPH and ORAC), glucose and fructose contents, and polyphenol profile of the extracts were determined. HPLE extracts obtained using high isopropanol concentrations (30%) and high temperatures (130 °C) presented the highest TPC (13.57 mg GAE/gdw) and antioxidant capacity (IC50: 9.97 mg/mL, ORAC: 246.47 µmol ET/gdw). Moreover, the use of 30% water–isopropanol resulted in higher yields of polyphenols and removal of reducing sugars compared to atmospheric extraction with water–acetone (60%). The polyphenolic profiles of the extracts showed that flavanols and phenolic acids were more soluble at high concentrations of isopropanol (30%). Contrarily, flavonols and stilbenes were better recovered with 15% isopropanol and pure water. Therefore, isopropanol could be a promising solvent for the selective recovery of different bioactive compounds from discarded blueberries and other agro-industrial residues.https://www.mdpi.com/2304-8158/12/19/3694discarded blueberriesisopropanolsubcritical conditionspolyphenolsantioxidant capacity |
| spellingShingle | Nils Leander Huamán-Castilla Cecilia Copa-Chipana Luis Omar Mamani-Apaza Olivia Magaly Luque-Vilca Clara Nely Campos-Quiróz Franz Zirena-Vilca María Salomé Mariotti-Celis Selective Recovery of Polyphenols from Discarded Blueberries (<i>Vaccinium corymbosum</i> L.) Using Hot Pressurized Liquid Extraction Combined with Isopropanol as an Environmentally Friendly Solvent Foods discarded blueberries isopropanol subcritical conditions polyphenols antioxidant capacity |
| title | Selective Recovery of Polyphenols from Discarded Blueberries (<i>Vaccinium corymbosum</i> L.) Using Hot Pressurized Liquid Extraction Combined with Isopropanol as an Environmentally Friendly Solvent |
| title_full | Selective Recovery of Polyphenols from Discarded Blueberries (<i>Vaccinium corymbosum</i> L.) Using Hot Pressurized Liquid Extraction Combined with Isopropanol as an Environmentally Friendly Solvent |
| title_fullStr | Selective Recovery of Polyphenols from Discarded Blueberries (<i>Vaccinium corymbosum</i> L.) Using Hot Pressurized Liquid Extraction Combined with Isopropanol as an Environmentally Friendly Solvent |
| title_full_unstemmed | Selective Recovery of Polyphenols from Discarded Blueberries (<i>Vaccinium corymbosum</i> L.) Using Hot Pressurized Liquid Extraction Combined with Isopropanol as an Environmentally Friendly Solvent |
| title_short | Selective Recovery of Polyphenols from Discarded Blueberries (<i>Vaccinium corymbosum</i> L.) Using Hot Pressurized Liquid Extraction Combined with Isopropanol as an Environmentally Friendly Solvent |
| title_sort | selective recovery of polyphenols from discarded blueberries i vaccinium corymbosum i l using hot pressurized liquid extraction combined with isopropanol as an environmentally friendly solvent |
| topic | discarded blueberries isopropanol subcritical conditions polyphenols antioxidant capacity |
| url | https://www.mdpi.com/2304-8158/12/19/3694 |
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