Intra-Arterially Delivered Mesenchymal Stem Cells Are Not Detected in the Brain Parenchyma in an Alzheimer's Disease Mouse Model.
Mesenchymal stem cells (MSCs) have a promising role as a therapeutic agent for neurodegenerative diseases such as Alzheimer's disease (AD). Prior studies suggested that intra-arterially administered MSCs are engrafted into the brain in stroke or traumatic brain injury (TBI) animal models. Howev...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4874686?pdf=render |
| _version_ | 1819177750151299072 |
|---|---|
| author | Na Kyung Lee Jehoon Yang Eun Hyuk Chang Sang Eon Park Jeongmin Lee Soo Jin Choi Wonil Oh Jong Wook Chang Duk L Na |
| author_facet | Na Kyung Lee Jehoon Yang Eun Hyuk Chang Sang Eon Park Jeongmin Lee Soo Jin Choi Wonil Oh Jong Wook Chang Duk L Na |
| author_sort | Na Kyung Lee |
| collection | DOAJ |
| description | Mesenchymal stem cells (MSCs) have a promising role as a therapeutic agent for neurodegenerative diseases such as Alzheimer's disease (AD). Prior studies suggested that intra-arterially administered MSCs are engrafted into the brain in stroke or traumatic brain injury (TBI) animal models. However, a controversial standpoint exists in terms of the integrity of the blood brain barrier (BBB) in transgenic AD mice. The primary goal of this study was to explore the feasibility of delivering human umbilical cord-blood derived mesenchymal stem cells (hUCB-MSCs) into the brains of non-transgenic WT (C3H/C57) and transgenic AD (APP/PS1) mice through the intra-arterial (IA) route. Through two experiments, mice were infused with hUCB-MSCs via the right internal carotid artery and were sacrificed at two different time points: 6 hours (experiment 1) or 5 minutes (experiment 2) after infusion. In both experiments, no cells were detected in the brain parenchyma while MSCs were detected in the cerebrovasculature in experiment 2. The results from this study highlight that intra-arterial delivery of MSCs is not the most favorable route to be implemented as a potential therapeutic approach for AD. |
| first_indexed | 2024-12-22T21:31:37Z |
| format | Article |
| id | doaj.art-5069380c3f5742108501660ab513df91 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T21:31:37Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-5069380c3f5742108501660ab513df912022-12-21T18:11:54ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01115e015591210.1371/journal.pone.0155912Intra-Arterially Delivered Mesenchymal Stem Cells Are Not Detected in the Brain Parenchyma in an Alzheimer's Disease Mouse Model.Na Kyung LeeJehoon YangEun Hyuk ChangSang Eon ParkJeongmin LeeSoo Jin ChoiWonil OhJong Wook ChangDuk L NaMesenchymal stem cells (MSCs) have a promising role as a therapeutic agent for neurodegenerative diseases such as Alzheimer's disease (AD). Prior studies suggested that intra-arterially administered MSCs are engrafted into the brain in stroke or traumatic brain injury (TBI) animal models. However, a controversial standpoint exists in terms of the integrity of the blood brain barrier (BBB) in transgenic AD mice. The primary goal of this study was to explore the feasibility of delivering human umbilical cord-blood derived mesenchymal stem cells (hUCB-MSCs) into the brains of non-transgenic WT (C3H/C57) and transgenic AD (APP/PS1) mice through the intra-arterial (IA) route. Through two experiments, mice were infused with hUCB-MSCs via the right internal carotid artery and were sacrificed at two different time points: 6 hours (experiment 1) or 5 minutes (experiment 2) after infusion. In both experiments, no cells were detected in the brain parenchyma while MSCs were detected in the cerebrovasculature in experiment 2. The results from this study highlight that intra-arterial delivery of MSCs is not the most favorable route to be implemented as a potential therapeutic approach for AD.http://europepmc.org/articles/PMC4874686?pdf=render |
| spellingShingle | Na Kyung Lee Jehoon Yang Eun Hyuk Chang Sang Eon Park Jeongmin Lee Soo Jin Choi Wonil Oh Jong Wook Chang Duk L Na Intra-Arterially Delivered Mesenchymal Stem Cells Are Not Detected in the Brain Parenchyma in an Alzheimer's Disease Mouse Model. PLoS ONE |
| title | Intra-Arterially Delivered Mesenchymal Stem Cells Are Not Detected in the Brain Parenchyma in an Alzheimer's Disease Mouse Model. |
| title_full | Intra-Arterially Delivered Mesenchymal Stem Cells Are Not Detected in the Brain Parenchyma in an Alzheimer's Disease Mouse Model. |
| title_fullStr | Intra-Arterially Delivered Mesenchymal Stem Cells Are Not Detected in the Brain Parenchyma in an Alzheimer's Disease Mouse Model. |
| title_full_unstemmed | Intra-Arterially Delivered Mesenchymal Stem Cells Are Not Detected in the Brain Parenchyma in an Alzheimer's Disease Mouse Model. |
| title_short | Intra-Arterially Delivered Mesenchymal Stem Cells Are Not Detected in the Brain Parenchyma in an Alzheimer's Disease Mouse Model. |
| title_sort | intra arterially delivered mesenchymal stem cells are not detected in the brain parenchyma in an alzheimer s disease mouse model |
| url | http://europepmc.org/articles/PMC4874686?pdf=render |
| work_keys_str_mv | AT nakyunglee intraarteriallydeliveredmesenchymalstemcellsarenotdetectedinthebrainparenchymainanalzheimersdiseasemousemodel AT jehoonyang intraarteriallydeliveredmesenchymalstemcellsarenotdetectedinthebrainparenchymainanalzheimersdiseasemousemodel AT eunhyukchang intraarteriallydeliveredmesenchymalstemcellsarenotdetectedinthebrainparenchymainanalzheimersdiseasemousemodel AT sangeonpark intraarteriallydeliveredmesenchymalstemcellsarenotdetectedinthebrainparenchymainanalzheimersdiseasemousemodel AT jeongminlee intraarteriallydeliveredmesenchymalstemcellsarenotdetectedinthebrainparenchymainanalzheimersdiseasemousemodel AT soojinchoi intraarteriallydeliveredmesenchymalstemcellsarenotdetectedinthebrainparenchymainanalzheimersdiseasemousemodel AT woniloh intraarteriallydeliveredmesenchymalstemcellsarenotdetectedinthebrainparenchymainanalzheimersdiseasemousemodel AT jongwookchang intraarteriallydeliveredmesenchymalstemcellsarenotdetectedinthebrainparenchymainanalzheimersdiseasemousemodel AT duklna intraarteriallydeliveredmesenchymalstemcellsarenotdetectedinthebrainparenchymainanalzheimersdiseasemousemodel |