Unnatural activities and mechanistic insights of cytochrome P450 PikC gained from site-specific mutagenesis by non-canonical amino acids
The 20 proteinogenic amino acids cannot always satisfy the requirements of protein engineering. Here, the authors practice semi-rational non-canonical amino acid mutagenesis for a cytochrome P450 enzyme, leading to unnatural activities and significant mechanistic insights.
Bibliographic Details
Main Authors: |
Yunjun Pan,
Guobang Li,
Ruxin Liu,
Jiawei Guo,
Yunjie Liu,
Mingyu Liu,
Xingwang Zhang,
Luping Chi,
Kangwei Xu,
Ruibo Wu,
Yuzhong Zhang,
Yuezhong Li,
Xiang Gao,
Shengying Li |
Format: | Article
|
Language: | English |
Published: |
Nature Portfolio
2023-03-01
|
Series: | Nature Communications
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Online Access: | https://doi.org/10.1038/s41467-023-37288-0
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