Moringa oleifera leaf alleviates functional constipation via regulating the gut microbiota and the enteric nervous system in mice
Moringa oleifera Lam. leaf is not only a new food resource in China, but also a traditional medicinal plant. It is commonly used in the folk to alleviate constipation, but its laxative mechanism is not fully understood. Hence we investigated it in loperamide-induced functional constipation (FC) mice...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1315402/full |
| _version_ | 1797384945401856000 |
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| author | Xiaoyu Gao Xiaoyu Gao Xiaoyu Gao Weiqian Yang Weiqian Yang Sijin Li Shuangfeng Liu Shuangfeng Liu Weixing Yang Shuang Song Jun Sheng Yan Zhao Yang Tian Yang Tian |
| author_facet | Xiaoyu Gao Xiaoyu Gao Xiaoyu Gao Weiqian Yang Weiqian Yang Sijin Li Shuangfeng Liu Shuangfeng Liu Weixing Yang Shuang Song Jun Sheng Yan Zhao Yang Tian Yang Tian |
| author_sort | Xiaoyu Gao |
| collection | DOAJ |
| description | Moringa oleifera Lam. leaf is not only a new food resource in China, but also a traditional medicinal plant. It is commonly used in the folk to alleviate constipation, but its laxative mechanism is not fully understood. Hence we investigated it in loperamide-induced functional constipation (FC) mice. The results showed that MOAE significantly regulated not only gastrointestinal hormones and neurotransmitters in serum but also important gastrointestinal motility factors in the enteric nervous system (ENS)-interstitial cells of Cajal (ICCs)-smooth muscle cell (SMC) network. Meanwhile, MOAE attenuated intestinal inflammation, increased cecal short-chain fatty acid levels and colonic antimicrobial peptide expression, and improved the impaired intestinal barrier function in loperamide-induced FC mice. In addition, MOAE also increased fecal water content by inhibiting the mRNA expression of colonic aquaporins (Aqp3 and Aqp4) in FC mice. Interestingly and importantly, MOAE affected the intestinal microbiota by inhibiting some key “constipation-causing” microbiota, such as Bacteroidaceae, Clostridiaceae, Bacteroides, and Ruminococcus, and promoting the growth of other important “constipation-curing” microbiota, such as Butyricoccus, Tyzzerella, and Desulfovibrio. These important taxa are significantly associated with a variety of indicators of constipation. These findings suggest that MOAE can promote defecation through its rich chemical composition to modulate the ENS-ICCs-SMCs network and the gut microecosystem. |
| first_indexed | 2024-03-08T21:45:58Z |
| format | Article |
| id | doaj.art-507163f6946449b8b13fa09009acc025 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T21:45:58Z |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-507163f6946449b8b13fa09009acc0252023-12-20T08:32:53ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-12-011410.3389/fmicb.2023.13154021315402Moringa oleifera leaf alleviates functional constipation via regulating the gut microbiota and the enteric nervous system in miceXiaoyu Gao0Xiaoyu Gao1Xiaoyu Gao2Weiqian Yang3Weiqian Yang4Sijin Li5Shuangfeng Liu6Shuangfeng Liu7Weixing Yang8Shuang Song9Jun Sheng10Yan Zhao11Yang Tian12Yang Tian13Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaEngineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaDepartment of Hotel Management, Chongqing Vocational Institute of Tourism, Chongqing, ChinaCollege of Pu’er Tea West Yunnan University of Applied Sciences, Puer, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaEngineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaCollege of Food Science and Technology, Yunnan Agricultural University, Kunming, ChinaEngineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaDivision of Science and Technology, Yunnan Agricultural University, Kunming, ChinaYunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming, ChinaEngineering Research Center of Development and Utilization of Food and Drug Homologous Resources, Ministry of Education, Yunnan Agricultural University, Kunming, ChinaMoringa oleifera Lam. leaf is not only a new food resource in China, but also a traditional medicinal plant. It is commonly used in the folk to alleviate constipation, but its laxative mechanism is not fully understood. Hence we investigated it in loperamide-induced functional constipation (FC) mice. The results showed that MOAE significantly regulated not only gastrointestinal hormones and neurotransmitters in serum but also important gastrointestinal motility factors in the enteric nervous system (ENS)-interstitial cells of Cajal (ICCs)-smooth muscle cell (SMC) network. Meanwhile, MOAE attenuated intestinal inflammation, increased cecal short-chain fatty acid levels and colonic antimicrobial peptide expression, and improved the impaired intestinal barrier function in loperamide-induced FC mice. In addition, MOAE also increased fecal water content by inhibiting the mRNA expression of colonic aquaporins (Aqp3 and Aqp4) in FC mice. Interestingly and importantly, MOAE affected the intestinal microbiota by inhibiting some key “constipation-causing” microbiota, such as Bacteroidaceae, Clostridiaceae, Bacteroides, and Ruminococcus, and promoting the growth of other important “constipation-curing” microbiota, such as Butyricoccus, Tyzzerella, and Desulfovibrio. These important taxa are significantly associated with a variety of indicators of constipation. These findings suggest that MOAE can promote defecation through its rich chemical composition to modulate the ENS-ICCs-SMCs network and the gut microecosystem.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1315402/fullintestinal motilitygut microbiotaBacteroidesButyricoccusRuminococcusDesulfovibrio |
| spellingShingle | Xiaoyu Gao Xiaoyu Gao Xiaoyu Gao Weiqian Yang Weiqian Yang Sijin Li Shuangfeng Liu Shuangfeng Liu Weixing Yang Shuang Song Jun Sheng Yan Zhao Yang Tian Yang Tian Moringa oleifera leaf alleviates functional constipation via regulating the gut microbiota and the enteric nervous system in mice Frontiers in Microbiology intestinal motility gut microbiota Bacteroides Butyricoccus Ruminococcus Desulfovibrio |
| title | Moringa oleifera leaf alleviates functional constipation via regulating the gut microbiota and the enteric nervous system in mice |
| title_full | Moringa oleifera leaf alleviates functional constipation via regulating the gut microbiota and the enteric nervous system in mice |
| title_fullStr | Moringa oleifera leaf alleviates functional constipation via regulating the gut microbiota and the enteric nervous system in mice |
| title_full_unstemmed | Moringa oleifera leaf alleviates functional constipation via regulating the gut microbiota and the enteric nervous system in mice |
| title_short | Moringa oleifera leaf alleviates functional constipation via regulating the gut microbiota and the enteric nervous system in mice |
| title_sort | moringa oleifera leaf alleviates functional constipation via regulating the gut microbiota and the enteric nervous system in mice |
| topic | intestinal motility gut microbiota Bacteroides Butyricoccus Ruminococcus Desulfovibrio |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1315402/full |
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