The Biological Effects of Polystyrene Nanoplastics on Human Peripheral Blood Lymphocytes
Environmental exposure to microplastics (MPs) and nanoplastics (NPs) is an increasing concern from human health perspectives. Little information on the genotoxic and cytotoxic potential of NP particles in human cells is available. We aimed to assess the cytotoxic and genotoxic potential of polystyre...
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MDPI AG
2022-05-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/10/1632 |
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| author | Devojit Kumar Sarma Ruchi Dubey Ravindra M. Samarth Swasti Shubham Pritom Chowdhury Manoj Kumawat Vinod Verma Rajnarayan R. Tiwari Manoj Kumar |
| author_facet | Devojit Kumar Sarma Ruchi Dubey Ravindra M. Samarth Swasti Shubham Pritom Chowdhury Manoj Kumawat Vinod Verma Rajnarayan R. Tiwari Manoj Kumar |
| author_sort | Devojit Kumar Sarma |
| collection | DOAJ |
| description | Environmental exposure to microplastics (MPs) and nanoplastics (NPs) is an increasing concern from human health perspectives. Little information on the genotoxic and cytotoxic potential of NP particles in human cells is available. We aimed to assess the cytotoxic and genotoxic potential of polystyrene nanoplastics (PSNPs) at different concentrations (2000μg/mL, 1000μg/mL, and 500μg/mL) by using chromosomal aberration (CA) and cytokinesis-block micronucleus assays (CBMN) on human peripheral lymphocytes. Dose-dependent hemolytic activity and cell viability were observed against the PSNPs exposure. Increased chromosomal aberrations, such as chromosomal breaks and dicentric chromosomes, and an increase in nucleoplasmic bridge (NBP) formation and nuclear budding (NBUD) were observed. The frequency of mitotic index (MI) decreased significantly in the PSNP-exposed groups from lower to higher concentrations. A significant increase in micronuclei (MN) formation and cytostasis% and a dose-dependent reduction in nuclear division index (NDI) in PSNP-exposed groups indicated oxidative stress-mediated cytotoxicity, DNA damage, and genomic instabilities due to PSNP exposure in human lymphocyte cells. This study highlights the importance of understanding the toxic mechanisms and associated chronic and acute health effects on humans due to exposure to this pervasive environmental pollutant. |
| first_indexed | 2024-03-10T03:17:30Z |
| format | Article |
| id | doaj.art-5073a2c623374f30a0db3ee187010795 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T03:17:30Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-5073a2c623374f30a0db3ee1870107952023-11-23T12:25:40ZengMDPI AGNanomaterials2079-49912022-05-011210163210.3390/nano12101632The Biological Effects of Polystyrene Nanoplastics on Human Peripheral Blood LymphocytesDevojit Kumar Sarma0Ruchi Dubey1Ravindra M. Samarth2Swasti Shubham3Pritom Chowdhury4Manoj Kumawat5Vinod Verma6Rajnarayan R. Tiwari7Manoj Kumar8ICMR—National Institute for Research in Environmental Health, Bhopal Bypass Road, Bhouri, Bhopal 462030, IndiaDepartment of Biotechnology, Barkatullah University, Bhopal 462026, IndiaDepartment of Research, ICMR—Bhopal Memorial Hospital & Research Centre, Bhopal 462038, IndiaICMR—National Institute for Research in Environmental Health, Bhopal Bypass Road, Bhouri, Bhopal 462030, IndiaDepartment of Biotechnology, Tocklai Tea Research Institute, Jorhat 785008, IndiaICMR—National Institute for Research in Environmental Health, Bhopal Bypass Road, Bhouri, Bhopal 462030, IndiaStem Cell Research Centre, Department of Hematology, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Lucknow 226014, IndiaICMR—National Institute for Research in Environmental Health, Bhopal Bypass Road, Bhouri, Bhopal 462030, IndiaICMR—National Institute for Research in Environmental Health, Bhopal Bypass Road, Bhouri, Bhopal 462030, IndiaEnvironmental exposure to microplastics (MPs) and nanoplastics (NPs) is an increasing concern from human health perspectives. Little information on the genotoxic and cytotoxic potential of NP particles in human cells is available. We aimed to assess the cytotoxic and genotoxic potential of polystyrene nanoplastics (PSNPs) at different concentrations (2000μg/mL, 1000μg/mL, and 500μg/mL) by using chromosomal aberration (CA) and cytokinesis-block micronucleus assays (CBMN) on human peripheral lymphocytes. Dose-dependent hemolytic activity and cell viability were observed against the PSNPs exposure. Increased chromosomal aberrations, such as chromosomal breaks and dicentric chromosomes, and an increase in nucleoplasmic bridge (NBP) formation and nuclear budding (NBUD) were observed. The frequency of mitotic index (MI) decreased significantly in the PSNP-exposed groups from lower to higher concentrations. A significant increase in micronuclei (MN) formation and cytostasis% and a dose-dependent reduction in nuclear division index (NDI) in PSNP-exposed groups indicated oxidative stress-mediated cytotoxicity, DNA damage, and genomic instabilities due to PSNP exposure in human lymphocyte cells. This study highlights the importance of understanding the toxic mechanisms and associated chronic and acute health effects on humans due to exposure to this pervasive environmental pollutant.https://www.mdpi.com/2079-4991/12/10/1632cytotoxicitychromosomal aberrationDNA damageenvironmental pollutantgenotoxicityhealth |
| spellingShingle | Devojit Kumar Sarma Ruchi Dubey Ravindra M. Samarth Swasti Shubham Pritom Chowdhury Manoj Kumawat Vinod Verma Rajnarayan R. Tiwari Manoj Kumar The Biological Effects of Polystyrene Nanoplastics on Human Peripheral Blood Lymphocytes Nanomaterials cytotoxicity chromosomal aberration DNA damage environmental pollutant genotoxicity health |
| title | The Biological Effects of Polystyrene Nanoplastics on Human Peripheral Blood Lymphocytes |
| title_full | The Biological Effects of Polystyrene Nanoplastics on Human Peripheral Blood Lymphocytes |
| title_fullStr | The Biological Effects of Polystyrene Nanoplastics on Human Peripheral Blood Lymphocytes |
| title_full_unstemmed | The Biological Effects of Polystyrene Nanoplastics on Human Peripheral Blood Lymphocytes |
| title_short | The Biological Effects of Polystyrene Nanoplastics on Human Peripheral Blood Lymphocytes |
| title_sort | biological effects of polystyrene nanoplastics on human peripheral blood lymphocytes |
| topic | cytotoxicity chromosomal aberration DNA damage environmental pollutant genotoxicity health |
| url | https://www.mdpi.com/2079-4991/12/10/1632 |
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