The in vitro Effect of Fibers With Different Degrees of Polymerization on Human Gut Bacteria
Human gut bacteria contribute significantly to human health and several studies have evaluated the effects of dietary fibers on human gut bacterial ecology. However, the relationship between different degrees of fiber polymerization and human gut bacteria is unknown. Here, we analyzed three fiber su...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-05-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.00819/full |
| _version_ | 1818578146757181440 |
|---|---|
| author | Miao Chen Bei Fan Bei Fan Shujun Liu Khandaker Md Sharif Uddin Imam Yingying Xie Boting Wen Fengjiao Xin |
| author_facet | Miao Chen Bei Fan Bei Fan Shujun Liu Khandaker Md Sharif Uddin Imam Yingying Xie Boting Wen Fengjiao Xin |
| author_sort | Miao Chen |
| collection | DOAJ |
| description | Human gut bacteria contribute significantly to human health and several studies have evaluated the effects of dietary fibers on human gut bacterial ecology. However, the relationship between different degrees of fiber polymerization and human gut bacteria is unknown. Here, we analyzed three fiber substrates with different degrees of polymerization, namely carboxymethylcellulose, β-glucans, and galactooligosaccharides. To probe the in vitro influence of the degree of polymerization of the fiber on human gut bacteria, we measured the pH, air pressure, and short-chain fatty acid content of fecal fermentation supplemented with these fiber substrates, and sequenced the 16S ribosomal RNA genes of the microbial community in the fiber-treated fermentations. The butyric acid concentration was shown to decline with decreasing degree of polymerization of the fiber. Illumina Miseq sequencing indicated that the degree of polymerization might have an influence on human gut microbial diversity and abundance. Principal coordinate analysis unveiled a relationship between the degree of fiber polymerization and the gut bacterial community. Specific microbiota operational taxonomic units (OTUs) within the genera Escherichia-Shigella, Fusobacterium, and Dorea were proportional to the degree of fiber significantly, whereas OTUs within the genera Bifidobacterium, Streptococcus, and Lactobacillus were inversely correlated with the degree of polymerization. Correlation analysis between the fiber degree of polymerization and gut bacteria may demonstrate the effect of fibers on gut microbiota, and subsequently, on human health. |
| first_indexed | 2024-12-16T06:41:10Z |
| format | Article |
| id | doaj.art-5075d1d1311f4f18af4e30ba4952b7f3 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-16T06:41:10Z |
| publishDate | 2020-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5075d1d1311f4f18af4e30ba4952b7f32022-12-21T22:40:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-05-011110.3389/fmicb.2020.00819521973The in vitro Effect of Fibers With Different Degrees of Polymerization on Human Gut BacteriaMiao Chen0Bei Fan1Bei Fan2Shujun Liu3Khandaker Md Sharif Uddin Imam4Yingying Xie5Boting Wen6Fengjiao Xin7Laboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, ChinaLaboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory of Biomanufacturing and Food Engineering, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, ChinaHuman gut bacteria contribute significantly to human health and several studies have evaluated the effects of dietary fibers on human gut bacterial ecology. However, the relationship between different degrees of fiber polymerization and human gut bacteria is unknown. Here, we analyzed three fiber substrates with different degrees of polymerization, namely carboxymethylcellulose, β-glucans, and galactooligosaccharides. To probe the in vitro influence of the degree of polymerization of the fiber on human gut bacteria, we measured the pH, air pressure, and short-chain fatty acid content of fecal fermentation supplemented with these fiber substrates, and sequenced the 16S ribosomal RNA genes of the microbial community in the fiber-treated fermentations. The butyric acid concentration was shown to decline with decreasing degree of polymerization of the fiber. Illumina Miseq sequencing indicated that the degree of polymerization might have an influence on human gut microbial diversity and abundance. Principal coordinate analysis unveiled a relationship between the degree of fiber polymerization and the gut bacterial community. Specific microbiota operational taxonomic units (OTUs) within the genera Escherichia-Shigella, Fusobacterium, and Dorea were proportional to the degree of fiber significantly, whereas OTUs within the genera Bifidobacterium, Streptococcus, and Lactobacillus were inversely correlated with the degree of polymerization. Correlation analysis between the fiber degree of polymerization and gut bacteria may demonstrate the effect of fibers on gut microbiota, and subsequently, on human health.https://www.frontiersin.org/article/10.3389/fmicb.2020.00819/fullin vitro fermentationpolymerization degreedietary fiberhuman gut bacteriashort-chain fatty acidsmicrobial communities |
| spellingShingle | Miao Chen Bei Fan Bei Fan Shujun Liu Khandaker Md Sharif Uddin Imam Yingying Xie Boting Wen Fengjiao Xin The in vitro Effect of Fibers With Different Degrees of Polymerization on Human Gut Bacteria Frontiers in Microbiology in vitro fermentation polymerization degree dietary fiber human gut bacteria short-chain fatty acids microbial communities |
| title | The in vitro Effect of Fibers With Different Degrees of Polymerization on Human Gut Bacteria |
| title_full | The in vitro Effect of Fibers With Different Degrees of Polymerization on Human Gut Bacteria |
| title_fullStr | The in vitro Effect of Fibers With Different Degrees of Polymerization on Human Gut Bacteria |
| title_full_unstemmed | The in vitro Effect of Fibers With Different Degrees of Polymerization on Human Gut Bacteria |
| title_short | The in vitro Effect of Fibers With Different Degrees of Polymerization on Human Gut Bacteria |
| title_sort | in vitro effect of fibers with different degrees of polymerization on human gut bacteria |
| topic | in vitro fermentation polymerization degree dietary fiber human gut bacteria short-chain fatty acids microbial communities |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.00819/full |
| work_keys_str_mv | AT miaochen theinvitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria AT beifan theinvitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria AT beifan theinvitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria AT shujunliu theinvitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria AT khandakermdsharifuddinimam theinvitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria AT yingyingxie theinvitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria AT botingwen theinvitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria AT fengjiaoxin theinvitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria AT miaochen invitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria AT beifan invitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria AT beifan invitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria AT shujunliu invitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria AT khandakermdsharifuddinimam invitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria AT yingyingxie invitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria AT botingwen invitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria AT fengjiaoxin invitroeffectoffiberswithdifferentdegreesofpolymerizationonhumangutbacteria |