Interaction of Prions Causes Heritable Traits in Saccharomyces cerevisiae.
The concept of "protein-based inheritance" defines prions as epigenetic determinants that cause several heritable traits in eukaryotic microorganisms, such as Saccharomyces cerevisiae and Podospora anserina. Previously, we discovered a non-chromosomal factor, [NSI+], which possesses the ma...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-12-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC5189945?pdf=render |
| _version_ | 1818545250782674944 |
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| author | Anton A Nizhnikov Tatyana A Ryzhova Kirill V Volkov Sergey P Zadorsky Julia V Sopova Sergey G Inge-Vechtomov Alexey P Galkin |
| author_facet | Anton A Nizhnikov Tatyana A Ryzhova Kirill V Volkov Sergey P Zadorsky Julia V Sopova Sergey G Inge-Vechtomov Alexey P Galkin |
| author_sort | Anton A Nizhnikov |
| collection | DOAJ |
| description | The concept of "protein-based inheritance" defines prions as epigenetic determinants that cause several heritable traits in eukaryotic microorganisms, such as Saccharomyces cerevisiae and Podospora anserina. Previously, we discovered a non-chromosomal factor, [NSI+], which possesses the main features of yeast prions, including cytoplasmic infectivity, reversible curability, dominance, and non-Mendelian inheritance in meiosis. This factor causes omnipotent suppression of nonsense mutations in strains of S. cerevisiae bearing a deleted or modified Sup35 N-terminal domain. In this work, we identified protein determinants of [NSI+] using an original method of proteomic screening for prions. The suppression of nonsense mutations in [NSI+] strains is determined by the interaction between [SWI+] and [PIN+] prions. Using genetic and biochemical methods, we showed that [SWI+] is the key determinant of this nonsense suppression, whereas [PIN+] does not cause nonsense suppression by itself but strongly enhances the effect of [SWI+]. We demonstrated that interaction of [SWI+] and [PIN+] causes inactivation of SUP45 gene that leads to nonsense suppression. Our data show that prion interactions may cause heritable traits in Saccharomyces cerevisiae. |
| first_indexed | 2024-12-12T01:59:02Z |
| format | Article |
| id | doaj.art-5077f65d33b6450c9689e4986ad3e0ce |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-12T01:59:02Z |
| publishDate | 2016-12-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-5077f65d33b6450c9689e4986ad3e0ce2022-12-22T00:42:15ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042016-12-011212e100650410.1371/journal.pgen.1006504Interaction of Prions Causes Heritable Traits in Saccharomyces cerevisiae.Anton A NizhnikovTatyana A RyzhovaKirill V VolkovSergey P ZadorskyJulia V SopovaSergey G Inge-VechtomovAlexey P GalkinThe concept of "protein-based inheritance" defines prions as epigenetic determinants that cause several heritable traits in eukaryotic microorganisms, such as Saccharomyces cerevisiae and Podospora anserina. Previously, we discovered a non-chromosomal factor, [NSI+], which possesses the main features of yeast prions, including cytoplasmic infectivity, reversible curability, dominance, and non-Mendelian inheritance in meiosis. This factor causes omnipotent suppression of nonsense mutations in strains of S. cerevisiae bearing a deleted or modified Sup35 N-terminal domain. In this work, we identified protein determinants of [NSI+] using an original method of proteomic screening for prions. The suppression of nonsense mutations in [NSI+] strains is determined by the interaction between [SWI+] and [PIN+] prions. Using genetic and biochemical methods, we showed that [SWI+] is the key determinant of this nonsense suppression, whereas [PIN+] does not cause nonsense suppression by itself but strongly enhances the effect of [SWI+]. We demonstrated that interaction of [SWI+] and [PIN+] causes inactivation of SUP45 gene that leads to nonsense suppression. Our data show that prion interactions may cause heritable traits in Saccharomyces cerevisiae.http://europepmc.org/articles/PMC5189945?pdf=render |
| spellingShingle | Anton A Nizhnikov Tatyana A Ryzhova Kirill V Volkov Sergey P Zadorsky Julia V Sopova Sergey G Inge-Vechtomov Alexey P Galkin Interaction of Prions Causes Heritable Traits in Saccharomyces cerevisiae. PLoS Genetics |
| title | Interaction of Prions Causes Heritable Traits in Saccharomyces cerevisiae. |
| title_full | Interaction of Prions Causes Heritable Traits in Saccharomyces cerevisiae. |
| title_fullStr | Interaction of Prions Causes Heritable Traits in Saccharomyces cerevisiae. |
| title_full_unstemmed | Interaction of Prions Causes Heritable Traits in Saccharomyces cerevisiae. |
| title_short | Interaction of Prions Causes Heritable Traits in Saccharomyces cerevisiae. |
| title_sort | interaction of prions causes heritable traits in saccharomyces cerevisiae |
| url | http://europepmc.org/articles/PMC5189945?pdf=render |
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