Anticancer activity of a sub-fraction of dichloromethane extract of <it>Strobilanthes crispus </it>on human breast and prostate cancer cells <it>in vitro</it>
<p>Abstract</p> <p>Background</p> <p>The leaves of <it>Strobilanthes crispus </it>(<it>S. crispus</it>) which is native to the regions of Madagascar to the Malay Archipelago, are used in folk medicine for their antidiabetic, diuretic, anticancer...
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BMC
2010-08-01
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| Series: | BMC Complementary and Alternative Medicine |
| Online Access: | http://www.biomedcentral.com/1472-6882/10/42 |
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| author | Navaratnam Visweswaran Lai Choon Zainal Abidin Siti Amalina Nik Mohamed Kamal Nik Nursyazni Hamzah Nurraihana Yaacob Nik Soriani Norazmi Mohd Nor |
| author_facet | Navaratnam Visweswaran Lai Choon Zainal Abidin Siti Amalina Nik Mohamed Kamal Nik Nursyazni Hamzah Nurraihana Yaacob Nik Soriani Norazmi Mohd Nor |
| author_sort | Navaratnam Visweswaran |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The leaves of <it>Strobilanthes crispus </it>(<it>S. crispus</it>) which is native to the regions of Madagascar to the Malay Archipelago, are used in folk medicine for their antidiabetic, diuretic, anticancer and blood pressure lowering properties. Crude extracts of this plant have been found to be cytotoxic to human cancer cell lines and protective against chemically-induced hepatocarcinogenesis in rats. In this study, the cytotoxicity of various sub-fractions of dichloromethane extract isolated from the leaves of <it>S. crispus </it>was determined and the anticancer activity of one of the bioactive sub-fractions, SC/D-F9, was further analysed in breast and prostate cancer cell lines.</p> <p>Methods</p> <p>The dichloromethane extract of <it>S. crispus </it>was chromatographed on silica gel by flash column chromatography. The ability of the various sub-fractions obtained to induce cell death of MCF-7, MDA-MB-231, PC-3 and DU-145 cell lines was determined using the LDH assay. The dose-response effect and the EC<sub>50 </sub>values of the active sub-fraction, SC/D-F9, were determined. Apoptosis was detected using Annexin V antibody and propidium iodide staining and analysed by fluorescence microscopy and flow cytometry, while caspase 3/7 activity was detected using FLICA caspase inhibitor and analysed by fluorescence microscopy.</p> <p>Results</p> <p>Selected sub-fractions of the dichloromethane extract induced death of MCF-7, MDA-MB-231, PC-3 and DU-145 cells. The sub-fraction SC/D-F9, consistently killed breast and prostate cancer cell lines with low EC<sub>50 </sub>values but is non-cytotoxic to the normal breast epithelial cell line, MCF-10A. SC/D-F9 displayed relatively higher cytotoxicity compared to tamoxifen, paclitaxel, docetaxel and doxorubicin. Cell death induced by SC/D-F9 occurred via apoptosis with the involvement of caspase 3 and/or 7.</p> <p>Conclusions</p> <p>A dichloromethane sub-fraction of <it>S. crispus </it>displayed potent anticancer activities <it>in vitro </it>that can be further exploited for the development of a potential therapeutic anticancer agent.</p> |
| first_indexed | 2024-12-11T13:21:07Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-11T13:21:07Z |
| publishDate | 2010-08-01 |
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| spelling | doaj.art-5078006c49824e7190f206a252e4fe042022-12-22T01:05:49ZengBMCBMC Complementary and Alternative Medicine1472-68822010-08-011014210.1186/1472-6882-10-42Anticancer activity of a sub-fraction of dichloromethane extract of <it>Strobilanthes crispus </it>on human breast and prostate cancer cells <it>in vitro</it>Navaratnam VisweswaranLai ChoonZainal Abidin Siti AmalinaNik Mohamed Kamal Nik NursyazniHamzah NurraihanaYaacob Nik SorianiNorazmi Mohd Nor<p>Abstract</p> <p>Background</p> <p>The leaves of <it>Strobilanthes crispus </it>(<it>S. crispus</it>) which is native to the regions of Madagascar to the Malay Archipelago, are used in folk medicine for their antidiabetic, diuretic, anticancer and blood pressure lowering properties. Crude extracts of this plant have been found to be cytotoxic to human cancer cell lines and protective against chemically-induced hepatocarcinogenesis in rats. In this study, the cytotoxicity of various sub-fractions of dichloromethane extract isolated from the leaves of <it>S. crispus </it>was determined and the anticancer activity of one of the bioactive sub-fractions, SC/D-F9, was further analysed in breast and prostate cancer cell lines.</p> <p>Methods</p> <p>The dichloromethane extract of <it>S. crispus </it>was chromatographed on silica gel by flash column chromatography. The ability of the various sub-fractions obtained to induce cell death of MCF-7, MDA-MB-231, PC-3 and DU-145 cell lines was determined using the LDH assay. The dose-response effect and the EC<sub>50 </sub>values of the active sub-fraction, SC/D-F9, were determined. Apoptosis was detected using Annexin V antibody and propidium iodide staining and analysed by fluorescence microscopy and flow cytometry, while caspase 3/7 activity was detected using FLICA caspase inhibitor and analysed by fluorescence microscopy.</p> <p>Results</p> <p>Selected sub-fractions of the dichloromethane extract induced death of MCF-7, MDA-MB-231, PC-3 and DU-145 cells. The sub-fraction SC/D-F9, consistently killed breast and prostate cancer cell lines with low EC<sub>50 </sub>values but is non-cytotoxic to the normal breast epithelial cell line, MCF-10A. SC/D-F9 displayed relatively higher cytotoxicity compared to tamoxifen, paclitaxel, docetaxel and doxorubicin. Cell death induced by SC/D-F9 occurred via apoptosis with the involvement of caspase 3 and/or 7.</p> <p>Conclusions</p> <p>A dichloromethane sub-fraction of <it>S. crispus </it>displayed potent anticancer activities <it>in vitro </it>that can be further exploited for the development of a potential therapeutic anticancer agent.</p>http://www.biomedcentral.com/1472-6882/10/42 |
| spellingShingle | Navaratnam Visweswaran Lai Choon Zainal Abidin Siti Amalina Nik Mohamed Kamal Nik Nursyazni Hamzah Nurraihana Yaacob Nik Soriani Norazmi Mohd Nor Anticancer activity of a sub-fraction of dichloromethane extract of <it>Strobilanthes crispus </it>on human breast and prostate cancer cells <it>in vitro</it> BMC Complementary and Alternative Medicine |
| title | Anticancer activity of a sub-fraction of dichloromethane extract of <it>Strobilanthes crispus </it>on human breast and prostate cancer cells <it>in vitro</it> |
| title_full | Anticancer activity of a sub-fraction of dichloromethane extract of <it>Strobilanthes crispus </it>on human breast and prostate cancer cells <it>in vitro</it> |
| title_fullStr | Anticancer activity of a sub-fraction of dichloromethane extract of <it>Strobilanthes crispus </it>on human breast and prostate cancer cells <it>in vitro</it> |
| title_full_unstemmed | Anticancer activity of a sub-fraction of dichloromethane extract of <it>Strobilanthes crispus </it>on human breast and prostate cancer cells <it>in vitro</it> |
| title_short | Anticancer activity of a sub-fraction of dichloromethane extract of <it>Strobilanthes crispus </it>on human breast and prostate cancer cells <it>in vitro</it> |
| title_sort | anticancer activity of a sub fraction of dichloromethane extract of it strobilanthes crispus it on human breast and prostate cancer cells it in vitro it |
| url | http://www.biomedcentral.com/1472-6882/10/42 |
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