Proteotypic Differences of Follicular-Patterned Thyroid Neoplasms
The diagnosis of follicular-patterned thyroid tumors such as follicular thyroid adenoma (FA), follicular thyroid carcinoma (FTC), and follicular variant of papillary thyroid carcinoma (FvPTC) remains challenging. This study aimed to explore the molecular differences among these three thyroid tumors...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.854611/full |
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| author | Dongdong Huang Huifang Zhang Lu Li Lu Li Lu Li Lu Li Weigang Ge Wei Liu Wei Liu Wei Liu Zhen Dong Zhen Dong Zhen Dong Jinlong Gao Jinlong Gao Jinlong Gao Nan Yao Wenxin Fu Lingling Huang Tiannan Guo Tiannan Guo Tiannan Guo Yaoting Sun Yaoting Sun Yaoting Sun Xiaodong Teng |
| author_facet | Dongdong Huang Huifang Zhang Lu Li Lu Li Lu Li Lu Li Weigang Ge Wei Liu Wei Liu Wei Liu Zhen Dong Zhen Dong Zhen Dong Jinlong Gao Jinlong Gao Jinlong Gao Nan Yao Wenxin Fu Lingling Huang Tiannan Guo Tiannan Guo Tiannan Guo Yaoting Sun Yaoting Sun Yaoting Sun Xiaodong Teng |
| author_sort | Dongdong Huang |
| collection | DOAJ |
| description | The diagnosis of follicular-patterned thyroid tumors such as follicular thyroid adenoma (FA), follicular thyroid carcinoma (FTC), and follicular variant of papillary thyroid carcinoma (FvPTC) remains challenging. This study aimed to explore the molecular differences among these three thyroid tumors by proteomic analysis. A pressure cycling technology (PCT)-data-independent acquisition (DIA) mass spectrometry workflow was employed to investigate protein alterations in 52 formalin-fixed paraffin-embedded (FFPE) specimens: 18 FA, 15 FTC, and 19 FvPTC specimens. Immunohistochemical (IHC) analysis of 101 FA, 67 FTC, and 65 FvPTC specimens and parallel reaction monitoring (PRM) analysis of 20 FA, 20 FTC, and 20 FvPTC specimens were performed to validate protein biomarkers. A total of 4107 proteins were quantified from 52 specimens. Pairwise comparisons identified 287 differentially regulated proteins between FTC and FA, and 303 between FvPTC and FA and 88 proteins were co-dysregulated in the two comparisons. However, only 23 discriminatory proteins between FTC and FvPTC were detected. Additionally, the quantitative results for ANXA1 expression based on IHC staining and PRM-MS quantification were consistent with the proteomic results, showing that ANXA1 can be used to distinguish FvPTC from FA and FTC. The differentially regulated proteins found in this study can differentiate FA from FvPTC. In addition, ANXA1 is a promising biomarker for differentiating FvPTC from the other thyroid tumors. |
| first_indexed | 2024-04-13T05:14:30Z |
| format | Article |
| id | doaj.art-507a55134292408fa5423567a69a2700 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-13T05:14:30Z |
| publishDate | 2022-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-507a55134292408fa5423567a69a27002022-12-22T03:00:55ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-07-011310.3389/fendo.2022.854611854611Proteotypic Differences of Follicular-Patterned Thyroid NeoplasmsDongdong Huang0Huifang Zhang1Lu Li2Lu Li3Lu Li4Lu Li5Weigang Ge6Wei Liu7Wei Liu8Wei Liu9Zhen Dong10Zhen Dong11Zhen Dong12Jinlong Gao13Jinlong Gao14Jinlong Gao15Nan Yao16Wenxin Fu17Lingling Huang18Tiannan Guo19Tiannan Guo20Tiannan Guo21Yaoting Sun22Yaoting Sun23Yaoting Sun24Xiaodong Teng25The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaThe First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaWestlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, ChinaInstitute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, ChinaResearch Center for Industries of the Future, Westlake University, Hangzhou, ChinaCollege of Pharmaceutical Sciences, Zhejiang University, Hangzhou, ChinaWestlake Omics (Hangzhou) Biotechnology Co., Ltd., Hangzhou, ChinaWestlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, ChinaInstitute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, ChinaResearch Center for Industries of the Future, Westlake University, Hangzhou, ChinaWestlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, ChinaInstitute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, ChinaResearch Center for Industries of the Future, Westlake University, Hangzhou, ChinaWestlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, ChinaInstitute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, ChinaResearch Center for Industries of the Future, Westlake University, Hangzhou, ChinaWestlake Omics (Hangzhou) Biotechnology Co., Ltd., Hangzhou, ChinaWestlake Omics (Hangzhou) Biotechnology Co., Ltd., Hangzhou, ChinaWestlake Omics (Hangzhou) Biotechnology Co., Ltd., Hangzhou, ChinaWestlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, ChinaInstitute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, ChinaResearch Center for Industries of the Future, Westlake University, Hangzhou, ChinaWestlake Laboratory of Life Sciences and Biomedicine, Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, ChinaInstitute of Basic Medical Sciences, Westlake Institute for Advanced Study, Hangzhou, ChinaResearch Center for Industries of the Future, Westlake University, Hangzhou, ChinaThe First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaThe diagnosis of follicular-patterned thyroid tumors such as follicular thyroid adenoma (FA), follicular thyroid carcinoma (FTC), and follicular variant of papillary thyroid carcinoma (FvPTC) remains challenging. This study aimed to explore the molecular differences among these three thyroid tumors by proteomic analysis. A pressure cycling technology (PCT)-data-independent acquisition (DIA) mass spectrometry workflow was employed to investigate protein alterations in 52 formalin-fixed paraffin-embedded (FFPE) specimens: 18 FA, 15 FTC, and 19 FvPTC specimens. Immunohistochemical (IHC) analysis of 101 FA, 67 FTC, and 65 FvPTC specimens and parallel reaction monitoring (PRM) analysis of 20 FA, 20 FTC, and 20 FvPTC specimens were performed to validate protein biomarkers. A total of 4107 proteins were quantified from 52 specimens. Pairwise comparisons identified 287 differentially regulated proteins between FTC and FA, and 303 between FvPTC and FA and 88 proteins were co-dysregulated in the two comparisons. However, only 23 discriminatory proteins between FTC and FvPTC were detected. Additionally, the quantitative results for ANXA1 expression based on IHC staining and PRM-MS quantification were consistent with the proteomic results, showing that ANXA1 can be used to distinguish FvPTC from FA and FTC. The differentially regulated proteins found in this study can differentiate FA from FvPTC. In addition, ANXA1 is a promising biomarker for differentiating FvPTC from the other thyroid tumors.https://www.frontiersin.org/articles/10.3389/fendo.2022.854611/fullfollicular thyroid adenomafollicular thyroid carcinomafollicular variant papillary thyroid carcinomadata-independent acquisitionmass spectrometry |
| spellingShingle | Dongdong Huang Huifang Zhang Lu Li Lu Li Lu Li Lu Li Weigang Ge Wei Liu Wei Liu Wei Liu Zhen Dong Zhen Dong Zhen Dong Jinlong Gao Jinlong Gao Jinlong Gao Nan Yao Wenxin Fu Lingling Huang Tiannan Guo Tiannan Guo Tiannan Guo Yaoting Sun Yaoting Sun Yaoting Sun Xiaodong Teng Proteotypic Differences of Follicular-Patterned Thyroid Neoplasms Frontiers in Endocrinology follicular thyroid adenoma follicular thyroid carcinoma follicular variant papillary thyroid carcinoma data-independent acquisition mass spectrometry |
| title | Proteotypic Differences of Follicular-Patterned Thyroid Neoplasms |
| title_full | Proteotypic Differences of Follicular-Patterned Thyroid Neoplasms |
| title_fullStr | Proteotypic Differences of Follicular-Patterned Thyroid Neoplasms |
| title_full_unstemmed | Proteotypic Differences of Follicular-Patterned Thyroid Neoplasms |
| title_short | Proteotypic Differences of Follicular-Patterned Thyroid Neoplasms |
| title_sort | proteotypic differences of follicular patterned thyroid neoplasms |
| topic | follicular thyroid adenoma follicular thyroid carcinoma follicular variant papillary thyroid carcinoma data-independent acquisition mass spectrometry |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.854611/full |
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