Alteration of m6A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice
The m6A methylation is reported to function in multiple physiological and pathological processes. However, the functional relevance of m6A modification to post-spinal cord injured (SCI) damage is not yet clear. In the present study, methylated RNA immunoprecipitation combined with microarray analysi...
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| Format: | Article |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2022.904573/full |
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| author | Shuangfei Ni Zixiang Luo Yonggang Fan Weixin Zhang Wei Peng Huafeng Zhang |
| author_facet | Shuangfei Ni Zixiang Luo Yonggang Fan Weixin Zhang Wei Peng Huafeng Zhang |
| author_sort | Shuangfei Ni |
| collection | DOAJ |
| description | The m6A methylation is reported to function in multiple physiological and pathological processes. However, the functional relevance of m6A modification to post-spinal cord injured (SCI) damage is not yet clear. In the present study, methylated RNA immunoprecipitation combined with microarray analysis showed that the global RNA m6A levels were decreased following SCI. Then, gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) analyses were conducted to demonstrate the potential function of differential m6A-tagged transcripts and the altered transcripts with differential m6A levels. In addition, we found that the m6A “writer,” METTL3, significantly decreased after SCI in mice. The immunostaining validated that the expression of METTL3 mainly changed in GFAP or Iba-1+ cells. Together, this study shows the alteration of m6A modification following SCI in mice, which might contribute to the pathophysiology of the spinal cord after trauma. |
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| format | Article |
| id | doaj.art-507c7a8c553640df9974bc6c108a991f |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-04-13T02:39:22Z |
| publishDate | 2022-08-01 |
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| spelling | doaj.art-507c7a8c553640df9974bc6c108a991f2022-12-22T03:06:15ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2022-08-011610.3389/fnins.2022.904573904573Alteration of m6A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in miceShuangfei Ni0Zixiang Luo1Yonggang Fan2Weixin Zhang3Wei Peng4Huafeng Zhang5Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Spine Surgery and Orthopaedics, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Orthopaedics, Zhejiang Chinese Medicine University, Hangzhou, ChinaDepartment of Spine Surgery and Orthopaedics, Xiangya Hospital, Central South University, Changsha, ChinaDepartment of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaThe m6A methylation is reported to function in multiple physiological and pathological processes. However, the functional relevance of m6A modification to post-spinal cord injured (SCI) damage is not yet clear. In the present study, methylated RNA immunoprecipitation combined with microarray analysis showed that the global RNA m6A levels were decreased following SCI. Then, gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) analyses were conducted to demonstrate the potential function of differential m6A-tagged transcripts and the altered transcripts with differential m6A levels. In addition, we found that the m6A “writer,” METTL3, significantly decreased after SCI in mice. The immunostaining validated that the expression of METTL3 mainly changed in GFAP or Iba-1+ cells. Together, this study shows the alteration of m6A modification following SCI in mice, which might contribute to the pathophysiology of the spinal cord after trauma.https://www.frontiersin.org/articles/10.3389/fnins.2022.904573/fullspinal cord injured (SCI)m6A (N6-methyladenosine)METTL3 (methyltransferase like 3)RNAmicroarray |
| spellingShingle | Shuangfei Ni Zixiang Luo Yonggang Fan Weixin Zhang Wei Peng Huafeng Zhang Alteration of m6A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice Frontiers in Neuroscience spinal cord injured (SCI) m6A (N6-methyladenosine) METTL3 (methyltransferase like 3) RNA microarray |
| title | Alteration of m6A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice |
| title_full | Alteration of m6A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice |
| title_fullStr | Alteration of m6A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice |
| title_full_unstemmed | Alteration of m6A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice |
| title_short | Alteration of m6A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice |
| title_sort | alteration of m6a epitranscriptomic tagging of ribonucleic acids after spinal cord injury in mice |
| topic | spinal cord injured (SCI) m6A (N6-methyladenosine) METTL3 (methyltransferase like 3) RNA microarray |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2022.904573/full |
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