Serum anti-α-crystallin antibodies in women with endocrine disorders
There is a distinct group among the patients with unexplained infertility who are found to have enhanced humoral immune response. Recent studies focus on the expression of stress proteins being an important factor in the stages of gametogenesis, fertilization, implantation, early embryonic developme...
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Format: | Article |
Language: | English |
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Taylor & Francis Group
2017-05-01
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Series: | Biotechnology & Biotechnological Equipment |
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Online Access: | http://dx.doi.org/10.1080/13102818.2017.1308232 |
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author | Iliana Buteva-Hristova Vladislav Lazarov Valentin Lozanov Antoaneta Gateva Blagovest Bechev Katerina Kavaldzieva Nikola Mladenov Nedka Trifonova Dimitrina Dimitrova-Dikanarova Zdravko Kamenov |
author_facet | Iliana Buteva-Hristova Vladislav Lazarov Valentin Lozanov Antoaneta Gateva Blagovest Bechev Katerina Kavaldzieva Nikola Mladenov Nedka Trifonova Dimitrina Dimitrova-Dikanarova Zdravko Kamenov |
author_sort | Iliana Buteva-Hristova |
collection | DOAJ |
description | There is a distinct group among the patients with unexplained infertility who are found to have enhanced humoral immune response. Recent studies focus on the expression of stress proteins being an important factor in the stages of gametogenesis, fertilization, implantation, early embryonic development and pregnancy. Increased expression of stress proteins and immune response against them was found in tissues exposed to stress. There is not enough data linking infertility to expression and immunity of α-crystallins in patients with endocrine diseases. The aim of this work was to study anti-α-crystallin antibodies in patients with polycystic ovary syndrome (PCOS), Graves’ disease, autoimmune thyroiditis, diabetes mellitus and obesity. Sera samples from 169 women with endocrine disorders (PCOS (n = 68); Graves’ disease (n = 26); autoimmune thyroiditis (n = 32); diabetes mellitus (n = 10); and obesity (n = 33)) were tested by ELISA. The statistical analysis was performed using SPSS program. The concentration of anti-α-crystallin antibodies is significantly elevated in the PCOS group compared to the control group (p = 0.021). The frequency of positive sera in the same group of patients is significantly higher compared to the control group (p = 0.029). In all other groups, no statistically significant elevation was observed. Elevated concentrations of anti-α-crystallin antibodies found in patients with PCOS suggest that the increased production of anti-α-crystallin antibodies in women with PCOS is most probably caused by failure of the immune tolerance and the induction of immune response. This is most likely due to an increased expression of this stress protein as a result of oxidative stress and chronic inflammation. |
first_indexed | 2024-12-19T07:15:46Z |
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id | doaj.art-507d9d1fda974ed183a9e663659b9d08 |
institution | Directory Open Access Journal |
issn | 1310-2818 1314-3530 |
language | English |
last_indexed | 2024-12-19T07:15:46Z |
publishDate | 2017-05-01 |
publisher | Taylor & Francis Group |
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series | Biotechnology & Biotechnological Equipment |
spelling | doaj.art-507d9d1fda974ed183a9e663659b9d082022-12-21T20:31:04ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302017-05-0131357458010.1080/13102818.2017.13082321308232Serum anti-α-crystallin antibodies in women with endocrine disordersIliana Buteva-Hristova0Vladislav Lazarov1Valentin Lozanov2Antoaneta Gateva3Blagovest Bechev4Katerina Kavaldzieva5Nikola Mladenov6Nedka Trifonova7Dimitrina Dimitrova-Dikanarova8Zdravko Kamenov9“St. Sofia” HospitalDepartment of Medical BiologyMedical University“Alexandrovska” HospitalMedical UniversityDepartment of Medical BiologyDepartment of Medical BiologyDepartment of Medical BiologyDepartment of Medical Biology“Alexandrovska” HospitalThere is a distinct group among the patients with unexplained infertility who are found to have enhanced humoral immune response. Recent studies focus on the expression of stress proteins being an important factor in the stages of gametogenesis, fertilization, implantation, early embryonic development and pregnancy. Increased expression of stress proteins and immune response against them was found in tissues exposed to stress. There is not enough data linking infertility to expression and immunity of α-crystallins in patients with endocrine diseases. The aim of this work was to study anti-α-crystallin antibodies in patients with polycystic ovary syndrome (PCOS), Graves’ disease, autoimmune thyroiditis, diabetes mellitus and obesity. Sera samples from 169 women with endocrine disorders (PCOS (n = 68); Graves’ disease (n = 26); autoimmune thyroiditis (n = 32); diabetes mellitus (n = 10); and obesity (n = 33)) were tested by ELISA. The statistical analysis was performed using SPSS program. The concentration of anti-α-crystallin antibodies is significantly elevated in the PCOS group compared to the control group (p = 0.021). The frequency of positive sera in the same group of patients is significantly higher compared to the control group (p = 0.029). In all other groups, no statistically significant elevation was observed. Elevated concentrations of anti-α-crystallin antibodies found in patients with PCOS suggest that the increased production of anti-α-crystallin antibodies in women with PCOS is most probably caused by failure of the immune tolerance and the induction of immune response. This is most likely due to an increased expression of this stress protein as a result of oxidative stress and chronic inflammation.http://dx.doi.org/10.1080/13102818.2017.1308232AutoantibodiessHSPα-crystallinpolycystic ovary syndrome (PCOS)autoimmune thyroiditis |
spellingShingle | Iliana Buteva-Hristova Vladislav Lazarov Valentin Lozanov Antoaneta Gateva Blagovest Bechev Katerina Kavaldzieva Nikola Mladenov Nedka Trifonova Dimitrina Dimitrova-Dikanarova Zdravko Kamenov Serum anti-α-crystallin antibodies in women with endocrine disorders Biotechnology & Biotechnological Equipment Autoantibodies sHSP α-crystallin polycystic ovary syndrome (PCOS) autoimmune thyroiditis |
title | Serum anti-α-crystallin antibodies in women with endocrine disorders |
title_full | Serum anti-α-crystallin antibodies in women with endocrine disorders |
title_fullStr | Serum anti-α-crystallin antibodies in women with endocrine disorders |
title_full_unstemmed | Serum anti-α-crystallin antibodies in women with endocrine disorders |
title_short | Serum anti-α-crystallin antibodies in women with endocrine disorders |
title_sort | serum anti α crystallin antibodies in women with endocrine disorders |
topic | Autoantibodies sHSP α-crystallin polycystic ovary syndrome (PCOS) autoimmune thyroiditis |
url | http://dx.doi.org/10.1080/13102818.2017.1308232 |
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