Frequency of Wra antigen and anti-Wra in Brazilian blood donors
BACKGROUND: Wra is a low-incidence antigen, which is antithetical to the high prevalence red blood cell antigen, Wrb. Anti-Wra is a naturally occurring antibody that is found in approximately 1-2% of blood donors. The aim of this study was to determine the frequency of Wra and anti-Wra in Brazilian...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-10-01
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| Series: | Revista Brasileira de Hematologia e Hemoterapia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842015000500316&lng=en&tlng=en |
| _version_ | 1819292193786953728 |
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| author | Janaína Guilhem Muniz Carine Prisco Arnoni Diana Gazito Rosangela de Medeiros Person Tatiana Aparecida de Paula Vendrame Flavia Roche Moreira Latini Lilian Castilho |
| author_facet | Janaína Guilhem Muniz Carine Prisco Arnoni Diana Gazito Rosangela de Medeiros Person Tatiana Aparecida de Paula Vendrame Flavia Roche Moreira Latini Lilian Castilho |
| author_sort | Janaína Guilhem Muniz |
| collection | DOAJ |
| description | BACKGROUND: Wra is a low-incidence antigen, which is antithetical to the high prevalence red blood cell antigen, Wrb. Anti-Wra is a naturally occurring antibody that is found in approximately 1-2% of blood donors. The aim of this study was to determine the frequency of Wra and anti-Wra in Brazilian blood donors.METHODS: A total of 1662 Brazilian blood donors were molecularly analyzed using the SNaPshot methodology to determine the WR*A/B alleles and to predict the frequency of the Wra antigen. To detect the anti-Wra, samples from 1049 blood donors were analyzed using a gel test with Wr(a+) red blood cells. The serum was treated with dithiothreitol (DTT) to determine the immunoglobulin classes. Immunoglobulin (Ig)-G isotype classification was performed in a gel test using the IgG1/IgG3 card. A monocyte monolayer assay was employed to predict the clinical significance of IgG anti-Wra.RESULTS: Of the 1662 donors, only one sample had the DI*02.03 allele in heterozygous predicting the Wr(a+b+) phenotype. Anti-Wra was detected in 34 (3.24%) samples, 64.7% in females and 35.3% in males. Regarding the immunoglobulin class, eight (23.5%) cases of anti-Wra were classified as IgG and 26 (76.5%) as IgM. Of the eight cases of IgG anti-Wra, four were IgG1, two were IgG3 and three anti-Wra were not IgG3 or IgG1, and thus probably IgG2 or IgG4. The results of the monocyte monolayer assay showed that IgG anti-Wra might be of clinical significance.CONCLUSION: This study shows a very low frequency (0.06%) of the Wra antigen in Brazilian blood donors. Additionally, it shows that the frequency of anti-Wra in this population is higher than previously reported. |
| first_indexed | 2024-12-24T03:50:39Z |
| format | Article |
| id | doaj.art-507ee272599e42c7be32c350724b5c0e |
| institution | Directory Open Access Journal |
| issn | 1806-0870 |
| language | English |
| last_indexed | 2024-12-24T03:50:39Z |
| publishDate | 2015-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Revista Brasileira de Hematologia e Hemoterapia |
| spelling | doaj.art-507ee272599e42c7be32c350724b5c0e2022-12-21T17:16:35ZengElsevierRevista Brasileira de Hematologia e Hemoterapia1806-08702015-10-0137531631910.1016/j.bjhh.2015.07.002S1516-84842015000500316Frequency of Wra antigen and anti-Wra in Brazilian blood donorsJanaína Guilhem MunizCarine Prisco ArnoniDiana GazitoRosangela de Medeiros PersonTatiana Aparecida de Paula VendrameFlavia Roche Moreira LatiniLilian CastilhoBACKGROUND: Wra is a low-incidence antigen, which is antithetical to the high prevalence red blood cell antigen, Wrb. Anti-Wra is a naturally occurring antibody that is found in approximately 1-2% of blood donors. The aim of this study was to determine the frequency of Wra and anti-Wra in Brazilian blood donors.METHODS: A total of 1662 Brazilian blood donors were molecularly analyzed using the SNaPshot methodology to determine the WR*A/B alleles and to predict the frequency of the Wra antigen. To detect the anti-Wra, samples from 1049 blood donors were analyzed using a gel test with Wr(a+) red blood cells. The serum was treated with dithiothreitol (DTT) to determine the immunoglobulin classes. Immunoglobulin (Ig)-G isotype classification was performed in a gel test using the IgG1/IgG3 card. A monocyte monolayer assay was employed to predict the clinical significance of IgG anti-Wra.RESULTS: Of the 1662 donors, only one sample had the DI*02.03 allele in heterozygous predicting the Wr(a+b+) phenotype. Anti-Wra was detected in 34 (3.24%) samples, 64.7% in females and 35.3% in males. Regarding the immunoglobulin class, eight (23.5%) cases of anti-Wra were classified as IgG and 26 (76.5%) as IgM. Of the eight cases of IgG anti-Wra, four were IgG1, two were IgG3 and three anti-Wra were not IgG3 or IgG1, and thus probably IgG2 or IgG4. The results of the monocyte monolayer assay showed that IgG anti-Wra might be of clinical significance.CONCLUSION: This study shows a very low frequency (0.06%) of the Wra antigen in Brazilian blood donors. Additionally, it shows that the frequency of anti-Wra in this population is higher than previously reported.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842015000500316&lng=en&tlng=enAnti-WraWra antigenDiego blood groupAllele frequency |
| spellingShingle | Janaína Guilhem Muniz Carine Prisco Arnoni Diana Gazito Rosangela de Medeiros Person Tatiana Aparecida de Paula Vendrame Flavia Roche Moreira Latini Lilian Castilho Frequency of Wra antigen and anti-Wra in Brazilian blood donors Revista Brasileira de Hematologia e Hemoterapia Anti-Wra Wra antigen Diego blood group Allele frequency |
| title | Frequency of Wra antigen and anti-Wra in Brazilian blood donors |
| title_full | Frequency of Wra antigen and anti-Wra in Brazilian blood donors |
| title_fullStr | Frequency of Wra antigen and anti-Wra in Brazilian blood donors |
| title_full_unstemmed | Frequency of Wra antigen and anti-Wra in Brazilian blood donors |
| title_short | Frequency of Wra antigen and anti-Wra in Brazilian blood donors |
| title_sort | frequency of wra antigen and anti wra in brazilian blood donors |
| topic | Anti-Wra Wra antigen Diego blood group Allele frequency |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842015000500316&lng=en&tlng=en |
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