Near-Infrared Fluorescence Imaging of Breast Cancer and Axillary Lymph Nodes After Intravenous Injection of Free Indocyanine Green
BackgroundNear-infrared fluorescence imaging (NIRFI) of breast cancer (BC) after the intravenous (IV) injection of free indocyanine green (fICG) has been reported to be feasible. However, some questions remained unclarified.ObjectiveTo evaluate the distribution of fICG in BC and the axillary lymph n...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2021-03-01
|
| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2021.602906/full |
| _version_ | 1818557365412167680 |
|---|---|
| author | Pierre Bourgeois Isabelle Veys Danielle Noterman Filip De Neubourg Marie Chintinne Sophie Vankerckhove Jean-Marie Nogaret |
| author_facet | Pierre Bourgeois Isabelle Veys Danielle Noterman Filip De Neubourg Marie Chintinne Sophie Vankerckhove Jean-Marie Nogaret |
| author_sort | Pierre Bourgeois |
| collection | DOAJ |
| description | BackgroundNear-infrared fluorescence imaging (NIRFI) of breast cancer (BC) after the intravenous (IV) injection of free indocyanine green (fICG) has been reported to be feasible. However, some questions remained unclarified.ObjectiveTo evaluate the distribution of fICG in BC and the axillary lymph nodes (LNs) of women undergoing surgery with complete axillary LN dissection (CALND) and/or selective lymphadenectomy (SLN) of sentinel LNs (NCT no. 01993576 and NCT no. 02027818).MethodsAn intravenous injection of fICG (0.25 mg/kg) was administered to one series of 20 women undergoing treatment with mastectomy, the day before surgery in 5 (group 1) and immediately before surgery in 15 (group 2: tumor localization, 25; and pN+ CALND, 4) as well as to another series of 20 women undergoing treatment with tumorectomy (group 3). A dedicated NIR camera was used for ex vivo fluorescence imaging of the 45 BC lesions and the LNs.ResultsIn group 1, two of the four BC lesions and one large pN+ LN exhibited fluorescence. In contrast, 24 of the 25 tumors in group 2 and all of the tumors in group 3 were fluorescent. The sentinel LNs were all fluorescent, as well as some of the LNs in all CALND specimens. Metastatic cells were found in the fluorescent LNs of the pN+ cases. Fluorescent BC lesions could be identified ex vivo on the surface of the lumpectomy specimen in 14 of 19 cases.ConclusionsWhen fICG is injected intravenously just before surgery, BC can be detected using NIRFI with high sensitivity, with metastatic axillary LNs also showing fluorescence. Such a technical approach seems promising in the management of BC and merits further investigation. |
| first_indexed | 2024-12-13T23:58:32Z |
| format | Article |
| id | doaj.art-50811e05a6964d9b8b802db5c45dded8 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-13T23:58:32Z |
| publishDate | 2021-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-50811e05a6964d9b8b802db5c45dded82022-12-21T23:26:27ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2021-03-011110.3389/fonc.2021.602906602906Near-Infrared Fluorescence Imaging of Breast Cancer and Axillary Lymph Nodes After Intravenous Injection of Free Indocyanine GreenPierre Bourgeois0Isabelle Veys1Danielle Noterman2Filip De Neubourg3Marie Chintinne4Sophie Vankerckhove5Jean-Marie Nogaret6Nuclear Medicine Service, Institut Jules Bordet, Université Libre de Bruxelles, Brussels, BelgiumSurgery Service, Institut Jules Bordet, Université Libre de Bruxelles, Brussels, BelgiumSurgery Service, Institut Jules Bordet, Université Libre de Bruxelles, Brussels, BelgiumSurgery Service, Institut Jules Bordet, Université Libre de Bruxelles, Brussels, BelgiumDepartment of Anatomo-Pathology, Institut Jules Bordet, Université Libre de Bruxelles, Brussels, BelgiumNuclear Medicine Service, Institut Jules Bordet, Université Libre de Bruxelles, Brussels, BelgiumSurgery Service, Institut Jules Bordet, Université Libre de Bruxelles, Brussels, BelgiumBackgroundNear-infrared fluorescence imaging (NIRFI) of breast cancer (BC) after the intravenous (IV) injection of free indocyanine green (fICG) has been reported to be feasible. However, some questions remained unclarified.ObjectiveTo evaluate the distribution of fICG in BC and the axillary lymph nodes (LNs) of women undergoing surgery with complete axillary LN dissection (CALND) and/or selective lymphadenectomy (SLN) of sentinel LNs (NCT no. 01993576 and NCT no. 02027818).MethodsAn intravenous injection of fICG (0.25 mg/kg) was administered to one series of 20 women undergoing treatment with mastectomy, the day before surgery in 5 (group 1) and immediately before surgery in 15 (group 2: tumor localization, 25; and pN+ CALND, 4) as well as to another series of 20 women undergoing treatment with tumorectomy (group 3). A dedicated NIR camera was used for ex vivo fluorescence imaging of the 45 BC lesions and the LNs.ResultsIn group 1, two of the four BC lesions and one large pN+ LN exhibited fluorescence. In contrast, 24 of the 25 tumors in group 2 and all of the tumors in group 3 were fluorescent. The sentinel LNs were all fluorescent, as well as some of the LNs in all CALND specimens. Metastatic cells were found in the fluorescent LNs of the pN+ cases. Fluorescent BC lesions could be identified ex vivo on the surface of the lumpectomy specimen in 14 of 19 cases.ConclusionsWhen fICG is injected intravenously just before surgery, BC can be detected using NIRFI with high sensitivity, with metastatic axillary LNs also showing fluorescence. Such a technical approach seems promising in the management of BC and merits further investigation.https://www.frontiersin.org/articles/10.3389/fonc.2021.602906/fullbreast canceraxillary lymph nodesindocyanine greenfluorescence imagingsentinel lymph nodes |
| spellingShingle | Pierre Bourgeois Isabelle Veys Danielle Noterman Filip De Neubourg Marie Chintinne Sophie Vankerckhove Jean-Marie Nogaret Near-Infrared Fluorescence Imaging of Breast Cancer and Axillary Lymph Nodes After Intravenous Injection of Free Indocyanine Green Frontiers in Oncology breast cancer axillary lymph nodes indocyanine green fluorescence imaging sentinel lymph nodes |
| title | Near-Infrared Fluorescence Imaging of Breast Cancer and Axillary Lymph Nodes After Intravenous Injection of Free Indocyanine Green |
| title_full | Near-Infrared Fluorescence Imaging of Breast Cancer and Axillary Lymph Nodes After Intravenous Injection of Free Indocyanine Green |
| title_fullStr | Near-Infrared Fluorescence Imaging of Breast Cancer and Axillary Lymph Nodes After Intravenous Injection of Free Indocyanine Green |
| title_full_unstemmed | Near-Infrared Fluorescence Imaging of Breast Cancer and Axillary Lymph Nodes After Intravenous Injection of Free Indocyanine Green |
| title_short | Near-Infrared Fluorescence Imaging of Breast Cancer and Axillary Lymph Nodes After Intravenous Injection of Free Indocyanine Green |
| title_sort | near infrared fluorescence imaging of breast cancer and axillary lymph nodes after intravenous injection of free indocyanine green |
| topic | breast cancer axillary lymph nodes indocyanine green fluorescence imaging sentinel lymph nodes |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2021.602906/full |
| work_keys_str_mv | AT pierrebourgeois nearinfraredfluorescenceimagingofbreastcancerandaxillarylymphnodesafterintravenousinjectionoffreeindocyaninegreen AT isabelleveys nearinfraredfluorescenceimagingofbreastcancerandaxillarylymphnodesafterintravenousinjectionoffreeindocyaninegreen AT daniellenoterman nearinfraredfluorescenceimagingofbreastcancerandaxillarylymphnodesafterintravenousinjectionoffreeindocyaninegreen AT filipdeneubourg nearinfraredfluorescenceimagingofbreastcancerandaxillarylymphnodesafterintravenousinjectionoffreeindocyaninegreen AT mariechintinne nearinfraredfluorescenceimagingofbreastcancerandaxillarylymphnodesafterintravenousinjectionoffreeindocyaninegreen AT sophievankerckhove nearinfraredfluorescenceimagingofbreastcancerandaxillarylymphnodesafterintravenousinjectionoffreeindocyaninegreen AT jeanmarienogaret nearinfraredfluorescenceimagingofbreastcancerandaxillarylymphnodesafterintravenousinjectionoffreeindocyaninegreen |