Autoimmune Hepatitis: Shifts in Gut Microbiota and Metabolic Pathways among Egyptian Patients
Autoimmune hepatitis (AIH) is a chronic inflammatory disorder with complex immunopathogenesis. Dysbiosis has been linked to many autoimmune diseases, but its detailed role in autoimmune hepatitis (AIH) still needs rigorous evaluation, especially in Egypt. We aimed to identify the shift in the gut mi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/7/1011 |
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| author | Nahla M. Elsherbiny Mohammed Rammadan Elham A. Hassan Mohamed E. Ali Abeer S. Abd El-Rehim Wael A. Abbas Mohamed A. A. Abozaid Ebtisam Hassanin Helal F. Hetta |
| author_facet | Nahla M. Elsherbiny Mohammed Rammadan Elham A. Hassan Mohamed E. Ali Abeer S. Abd El-Rehim Wael A. Abbas Mohamed A. A. Abozaid Ebtisam Hassanin Helal F. Hetta |
| author_sort | Nahla M. Elsherbiny |
| collection | DOAJ |
| description | Autoimmune hepatitis (AIH) is a chronic inflammatory disorder with complex immunopathogenesis. Dysbiosis has been linked to many autoimmune diseases, but its detailed role in autoimmune hepatitis (AIH) still needs rigorous evaluation, especially in Egypt. We aimed to identify the shift in the gut microbiota profile and resultant metabolic pathways in AIH Egyptian patients compared to healthy individuals. Stool samples were collected from 15 AIH-naive patients and from 10 healthy individuals. The V3-V4 hyper-variable regions in16S rRNA gene was amplified and sequenced using Illumina MiSeq platform. Significantly lower bacterial diversity in AIH patients was found compared to the controls. A phylum-level analysis showed the overrepresentation of Firmicutes, Bacteroides, and Proteobacteria. At the genus level, AIH-associated enrichment of <i>Faecalibacterium</i>, <i>Blautia</i>, <i>Streptococcus</i>, <i>Haemophilus, Bacteroides</i>, <i>Veillonella</i>, <i>Eubacterium</i>, <i>Lachnospiraceae</i> and <i>Butyricicoccus</i> was reported in contrast to <i>Prevotella</i>, <i>Parabacteroides</i> and <i>Dilaster</i>, which were significantly retracted in such patients. Overall, the predicted metabolic pathways associated with dysbiosis in AIH patients could orchestrate the potential pathogenic roles of gut microbiota in autoimmune disease, though not in a disease-specific manner, calling for future large-scale studies. |
| first_indexed | 2024-03-10T18:38:25Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T18:38:25Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-5081c1db5fe94b7b994a11d3b79175e12023-11-20T06:00:35ZengMDPI AGMicroorganisms2076-26072020-07-0187101110.3390/microorganisms8071011Autoimmune Hepatitis: Shifts in Gut Microbiota and Metabolic Pathways among Egyptian PatientsNahla M. Elsherbiny0Mohammed Rammadan1Elham A. Hassan2Mohamed E. Ali3Abeer S. Abd El-Rehim4Wael A. Abbas5Mohamed A. A. Abozaid6Ebtisam Hassanin7Helal F. Hetta8Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut 71515, EgyptMicrobiology and Immunology Department, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, EgyptGastroenterology and Tropical Medicine Department, Faculty of Medicine, Assiut University, Assiut 71515, EgyptMicrobiology and Immunology Department, Faculty of Pharmacy, Al-Azhar University, Assiut 71524, EgyptGastroenterology and Tropical Medicine Department, Faculty of Medicine, Assiut University, Assiut 71515, EgyptInternal Medicine Department, Gastroenterology and Hepatology Unit, Faculty of Medicine, Assiut University, Assiut 71515, EgyptInternal Medicine Department, Gastroenterology and Hepatology Unit, Faculty of Medicine, Assiut University, Assiut 71515, EgyptClinical Pathology, Ministry of Health, Assiut 71511, EgyptMedical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut 71515, EgyptAutoimmune hepatitis (AIH) is a chronic inflammatory disorder with complex immunopathogenesis. Dysbiosis has been linked to many autoimmune diseases, but its detailed role in autoimmune hepatitis (AIH) still needs rigorous evaluation, especially in Egypt. We aimed to identify the shift in the gut microbiota profile and resultant metabolic pathways in AIH Egyptian patients compared to healthy individuals. Stool samples were collected from 15 AIH-naive patients and from 10 healthy individuals. The V3-V4 hyper-variable regions in16S rRNA gene was amplified and sequenced using Illumina MiSeq platform. Significantly lower bacterial diversity in AIH patients was found compared to the controls. A phylum-level analysis showed the overrepresentation of Firmicutes, Bacteroides, and Proteobacteria. At the genus level, AIH-associated enrichment of <i>Faecalibacterium</i>, <i>Blautia</i>, <i>Streptococcus</i>, <i>Haemophilus, Bacteroides</i>, <i>Veillonella</i>, <i>Eubacterium</i>, <i>Lachnospiraceae</i> and <i>Butyricicoccus</i> was reported in contrast to <i>Prevotella</i>, <i>Parabacteroides</i> and <i>Dilaster</i>, which were significantly retracted in such patients. Overall, the predicted metabolic pathways associated with dysbiosis in AIH patients could orchestrate the potential pathogenic roles of gut microbiota in autoimmune disease, though not in a disease-specific manner, calling for future large-scale studies.https://www.mdpi.com/2076-2607/8/7/1011bioinformaticsmicrobiomeautoimmune hepatitis |
| spellingShingle | Nahla M. Elsherbiny Mohammed Rammadan Elham A. Hassan Mohamed E. Ali Abeer S. Abd El-Rehim Wael A. Abbas Mohamed A. A. Abozaid Ebtisam Hassanin Helal F. Hetta Autoimmune Hepatitis: Shifts in Gut Microbiota and Metabolic Pathways among Egyptian Patients Microorganisms bioinformatics microbiome autoimmune hepatitis |
| title | Autoimmune Hepatitis: Shifts in Gut Microbiota and Metabolic Pathways among Egyptian Patients |
| title_full | Autoimmune Hepatitis: Shifts in Gut Microbiota and Metabolic Pathways among Egyptian Patients |
| title_fullStr | Autoimmune Hepatitis: Shifts in Gut Microbiota and Metabolic Pathways among Egyptian Patients |
| title_full_unstemmed | Autoimmune Hepatitis: Shifts in Gut Microbiota and Metabolic Pathways among Egyptian Patients |
| title_short | Autoimmune Hepatitis: Shifts in Gut Microbiota and Metabolic Pathways among Egyptian Patients |
| title_sort | autoimmune hepatitis shifts in gut microbiota and metabolic pathways among egyptian patients |
| topic | bioinformatics microbiome autoimmune hepatitis |
| url | https://www.mdpi.com/2076-2607/8/7/1011 |
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