Enrichment and characterization of Anammox bacteria in a non-woven membrane reactor
An upflow column reactor packed with nonwoven fabric carrier was used to enrich anaerobic ammonium oxidation (Anammox) sludge. After 101 days, the reactor Anammox sludge concentration increased from 470 to 3,118 mg·L−1. In the stable operating stage, the average total nitrogen (TN) volume loading ra...
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| Format: | Article |
| Language: | English |
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IWA Publishing
2022-03-01
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| Series: | Water Practice and Technology |
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| Online Access: | http://wpt.iwaponline.com/content/17/3/798 |
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| author | Yuhua Lyu Song Yan Zheng Gong Shoutong Zhang |
| author_facet | Yuhua Lyu Song Yan Zheng Gong Shoutong Zhang |
| author_sort | Yuhua Lyu |
| collection | DOAJ |
| description | An upflow column reactor packed with nonwoven fabric carrier was used to enrich anaerobic ammonium oxidation (Anammox) sludge. After 101 days, the reactor Anammox sludge concentration increased from 470 to 3,118 mg·L−1. In the stable operating stage, the average total nitrogen (TN) volume loading rate was 818.3 mg·L−1, and the maximum removal efficiencies of NH4+-N, NO2−-N and TN were 65.9, 81.2 and 63.8%, respectively. Scanning electron microscopy (SEM) showed that the cultivated sludge was dominated by a mix of short rod-shaped and spherical bacteria, which accumulated to the typical cauliflower-like aggregates assumed to be the Anammox culture. Fluorescence in situ hybridization (FISH) analysis using 16S rRNA showed that the dominant population developed in the reactor when hybridized with both PLA46 and Amx820 gene probes. This indicates that the cultivated biomass may comprise Planctomycetes bacteria. The results of real-time quantitative PCR (qPCR) showed that these bacteria formed 45 to 60% of the total bacteria in the Anammox sludge. The study demonstrated successful detection and enrichment of Anammox bacteria in wastewater. HIGHLIGHTS
Anammox bacteria was successfully enriched by a designed non-woven membrane reactor.;
The maximum TN removal rate was 63.8% in stable operation.;
The SEM and FISH analysis showed Anammox bacteria coexisted with the others as the dominant population.;
The qPCR results showed a 45% to 60% proportion of Planctomycetes in the culture.; |
| first_indexed | 2024-04-13T08:58:39Z |
| format | Article |
| id | doaj.art-5082559751c34a0c961a5a3a9fb34234 |
| institution | Directory Open Access Journal |
| issn | 1751-231X |
| language | English |
| last_indexed | 2024-04-13T08:58:39Z |
| publishDate | 2022-03-01 |
| publisher | IWA Publishing |
| record_format | Article |
| series | Water Practice and Technology |
| spelling | doaj.art-5082559751c34a0c961a5a3a9fb342342022-12-22T02:53:11ZengIWA PublishingWater Practice and Technology1751-231X2022-03-0117379880710.2166/wpt.2022.021021Enrichment and characterization of Anammox bacteria in a non-woven membrane reactorYuhua Lyu0Song Yan1Zheng Gong2Shoutong Zhang3 School of Environmental and Chemical Engineering, Dalian Jiaotong University, 794 Huanghe Road, Shahekou District, Dalian 116028, China School of Environmental and Chemical Engineering, Dalian Jiaotong University, 794 Huanghe Road, Shahekou District, Dalian 116028, China School of Life Science, Liaoning Normal University, 850 Huanghe Road, Shahekou District, Dalian 116081, China School of Environmental and Chemical Engineering, Dalian Jiaotong University, 794 Huanghe Road, Shahekou District, Dalian 116028, China An upflow column reactor packed with nonwoven fabric carrier was used to enrich anaerobic ammonium oxidation (Anammox) sludge. After 101 days, the reactor Anammox sludge concentration increased from 470 to 3,118 mg·L−1. In the stable operating stage, the average total nitrogen (TN) volume loading rate was 818.3 mg·L−1, and the maximum removal efficiencies of NH4+-N, NO2−-N and TN were 65.9, 81.2 and 63.8%, respectively. Scanning electron microscopy (SEM) showed that the cultivated sludge was dominated by a mix of short rod-shaped and spherical bacteria, which accumulated to the typical cauliflower-like aggregates assumed to be the Anammox culture. Fluorescence in situ hybridization (FISH) analysis using 16S rRNA showed that the dominant population developed in the reactor when hybridized with both PLA46 and Amx820 gene probes. This indicates that the cultivated biomass may comprise Planctomycetes bacteria. The results of real-time quantitative PCR (qPCR) showed that these bacteria formed 45 to 60% of the total bacteria in the Anammox sludge. The study demonstrated successful detection and enrichment of Anammox bacteria in wastewater. HIGHLIGHTS Anammox bacteria was successfully enriched by a designed non-woven membrane reactor.; The maximum TN removal rate was 63.8% in stable operation.; The SEM and FISH analysis showed Anammox bacteria coexisted with the others as the dominant population.; The qPCR results showed a 45% to 60% proportion of Planctomycetes in the culture.;http://wpt.iwaponline.com/content/17/3/798anammox bacteriaenrichmentfishnonwoven fabric carrierqpcrsem |
| spellingShingle | Yuhua Lyu Song Yan Zheng Gong Shoutong Zhang Enrichment and characterization of Anammox bacteria in a non-woven membrane reactor Water Practice and Technology anammox bacteria enrichment fish nonwoven fabric carrier qpcr sem |
| title | Enrichment and characterization of Anammox bacteria in a non-woven membrane reactor |
| title_full | Enrichment and characterization of Anammox bacteria in a non-woven membrane reactor |
| title_fullStr | Enrichment and characterization of Anammox bacteria in a non-woven membrane reactor |
| title_full_unstemmed | Enrichment and characterization of Anammox bacteria in a non-woven membrane reactor |
| title_short | Enrichment and characterization of Anammox bacteria in a non-woven membrane reactor |
| title_sort | enrichment and characterization of anammox bacteria in a non woven membrane reactor |
| topic | anammox bacteria enrichment fish nonwoven fabric carrier qpcr sem |
| url | http://wpt.iwaponline.com/content/17/3/798 |
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