Lactiplantibacillus plantarum 0111 Protects Against Influenza Virus by Modulating Intestinal Microbial-Mediated Immune Responses
There are some limitations of traditional influenza vaccines concerning novel mutant strains. Therefore, it is particularly important to develop preventive means for antigen-unrelated types of influenza viruses. Recent studies have shown that probiotics can modulate the immune system and reduce the...
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.820484/full |
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| author | Jun-Hong Xing Chun-Wei Shi Ming-Jie Sun Wei Gu Rong-Rong Zhang Hong-Liang Chen Ying Li Dan Wang JunYi Li Tian-Ming Niu Qun-Tao Huang Jia-Hao Qian Hai Bin Huang Yan-Long Jiang Jian-Zhong Wang Xin Cao Nan Wang Yan Zeng Gui-Lian Yang Wen Tao Yang Chun-Feng Wang |
| author_facet | Jun-Hong Xing Chun-Wei Shi Ming-Jie Sun Wei Gu Rong-Rong Zhang Hong-Liang Chen Ying Li Dan Wang JunYi Li Tian-Ming Niu Qun-Tao Huang Jia-Hao Qian Hai Bin Huang Yan-Long Jiang Jian-Zhong Wang Xin Cao Nan Wang Yan Zeng Gui-Lian Yang Wen Tao Yang Chun-Feng Wang |
| author_sort | Jun-Hong Xing |
| collection | DOAJ |
| description | There are some limitations of traditional influenza vaccines concerning novel mutant strains. Therefore, it is particularly important to develop preventive means for antigen-unrelated types of influenza viruses. Recent studies have shown that probiotics can modulate the immune system and reduce the severity of viral infections. In this study, we investigated the potential of Lactiplantibacillus plantarum 0111 against influenza virus H9N2. Challenge experiments showed that L. plantarum 0111 pretreatments could effectively improve mice’s survival rate and weight loss and reduce the inflammatory cytokines IL-6 and TNF-α in the lungs and bronchoalveolar lavage fluid (BALF) along with the degree of lung and intestinal injury. FMT experiment demonstrates that the protective effect produced by L. plantarum 0111 is associated with gut microorganisms. In addition, 16S high-throughput sequencing of the mouse intestinal microbiota showed that L. plantarum 0111 remodeled the intestinal microbiota after H9N2 infection and maintained the gut microbiota balance. In a mouse model, the oral administration of L. plantarum 0111 increased IFN-β expression in the serum and BALF. At the same time, the transcript levels of IFN-β and related ISGs in the intestine and lungs of mice were also increased. In addition, the activation and polarization of T cells in mesenteric lymph nodes (MLNs) and the spleen were detected by flow cytometry, and the results showed that L. plantarum 0111 modulated cytokines in T cells and increased IgA expression in B cells in the MLNs and spleen. Thus, L. plantarum 0111 may improve gut microbiota-mediated immune responses and thus, resist infection by the influenza virus, and it could be used as an effective preventive measure against the influenza virus. |
| first_indexed | 2024-12-12T13:50:58Z |
| format | Article |
| id | doaj.art-50842096129f437ba07e9a189dfa89fe |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T13:50:58Z |
| publishDate | 2022-06-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-50842096129f437ba07e9a189dfa89fe2022-12-22T00:22:33ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.820484820484Lactiplantibacillus plantarum 0111 Protects Against Influenza Virus by Modulating Intestinal Microbial-Mediated Immune ResponsesJun-Hong Xing0Chun-Wei Shi1Ming-Jie Sun2Wei Gu3Rong-Rong Zhang4Hong-Liang Chen5Ying Li6Dan Wang7JunYi Li8Tian-Ming Niu9Qun-Tao Huang10Jia-Hao Qian11Hai Bin Huang12Yan-Long Jiang13Jian-Zhong Wang14Xin Cao15Nan Wang16Yan Zeng17Gui-Lian Yang18Wen Tao Yang19Chun-Feng Wang20Key Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaShandong BaoLai-LeeLai Bioengineering Co., Ltd., Tai’an, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaKey Laboratory of Animal Production and Product Quality Safety of Ministry of Education, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Provincial Engineering Research Center of Animal Probiotics, Jilin Agricultural University, Changchun, ChinaThere are some limitations of traditional influenza vaccines concerning novel mutant strains. Therefore, it is particularly important to develop preventive means for antigen-unrelated types of influenza viruses. Recent studies have shown that probiotics can modulate the immune system and reduce the severity of viral infections. In this study, we investigated the potential of Lactiplantibacillus plantarum 0111 against influenza virus H9N2. Challenge experiments showed that L. plantarum 0111 pretreatments could effectively improve mice’s survival rate and weight loss and reduce the inflammatory cytokines IL-6 and TNF-α in the lungs and bronchoalveolar lavage fluid (BALF) along with the degree of lung and intestinal injury. FMT experiment demonstrates that the protective effect produced by L. plantarum 0111 is associated with gut microorganisms. In addition, 16S high-throughput sequencing of the mouse intestinal microbiota showed that L. plantarum 0111 remodeled the intestinal microbiota after H9N2 infection and maintained the gut microbiota balance. In a mouse model, the oral administration of L. plantarum 0111 increased IFN-β expression in the serum and BALF. At the same time, the transcript levels of IFN-β and related ISGs in the intestine and lungs of mice were also increased. In addition, the activation and polarization of T cells in mesenteric lymph nodes (MLNs) and the spleen were detected by flow cytometry, and the results showed that L. plantarum 0111 modulated cytokines in T cells and increased IgA expression in B cells in the MLNs and spleen. Thus, L. plantarum 0111 may improve gut microbiota-mediated immune responses and thus, resist infection by the influenza virus, and it could be used as an effective preventive measure against the influenza virus.https://www.frontiersin.org/articles/10.3389/fmicb.2022.820484/fullprobioticsinfluenza virusIFN-βT cellsgut microbiota |
| spellingShingle | Jun-Hong Xing Chun-Wei Shi Ming-Jie Sun Wei Gu Rong-Rong Zhang Hong-Liang Chen Ying Li Dan Wang JunYi Li Tian-Ming Niu Qun-Tao Huang Jia-Hao Qian Hai Bin Huang Yan-Long Jiang Jian-Zhong Wang Xin Cao Nan Wang Yan Zeng Gui-Lian Yang Wen Tao Yang Chun-Feng Wang Lactiplantibacillus plantarum 0111 Protects Against Influenza Virus by Modulating Intestinal Microbial-Mediated Immune Responses Frontiers in Microbiology probiotics influenza virus IFN-β T cells gut microbiota |
| title | Lactiplantibacillus plantarum 0111 Protects Against Influenza Virus by Modulating Intestinal Microbial-Mediated Immune Responses |
| title_full | Lactiplantibacillus plantarum 0111 Protects Against Influenza Virus by Modulating Intestinal Microbial-Mediated Immune Responses |
| title_fullStr | Lactiplantibacillus plantarum 0111 Protects Against Influenza Virus by Modulating Intestinal Microbial-Mediated Immune Responses |
| title_full_unstemmed | Lactiplantibacillus plantarum 0111 Protects Against Influenza Virus by Modulating Intestinal Microbial-Mediated Immune Responses |
| title_short | Lactiplantibacillus plantarum 0111 Protects Against Influenza Virus by Modulating Intestinal Microbial-Mediated Immune Responses |
| title_sort | lactiplantibacillus plantarum 0111 protects against influenza virus by modulating intestinal microbial mediated immune responses |
| topic | probiotics influenza virus IFN-β T cells gut microbiota |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.820484/full |
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