Morphology, Phenotype, and Molecular Identification of Clinical and Environmental <i>Fusarium solani</i> Species Complex Isolates from Malaysia
<i>Fusarium</i> infections in humans (fusariosis) and in economically important plants involve species of several <i>Fusarium</i> species complexes. Species of the <i>Fusarium solani</i> species complex (FSSC) are the most frequent cause of human fusariosis. The F...
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MDPI AG
2022-08-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/8/845 |
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| author | Jasper E. James Jacinta Santhanam Latiffah Zakaria Nuraini Mamat Rusli Mariahyati Abu Bakar Satinee Suetrong Jariya Sakayaroj Mohd Fuat Abdul Razak Erwin Lamping Richard D. Cannon |
| author_facet | Jasper E. James Jacinta Santhanam Latiffah Zakaria Nuraini Mamat Rusli Mariahyati Abu Bakar Satinee Suetrong Jariya Sakayaroj Mohd Fuat Abdul Razak Erwin Lamping Richard D. Cannon |
| author_sort | Jasper E. James |
| collection | DOAJ |
| description | <i>Fusarium</i> infections in humans (fusariosis) and in economically important plants involve species of several <i>Fusarium</i> species complexes. Species of the <i>Fusarium solani</i> species complex (FSSC) are the most frequent cause of human fusariosis. The FSSC comprises more than 60 closely related species that can be separated into three major clades by multi-locus sequence typing (MLST) using translation elongation factor 1-alpha (<i>TEF1-α</i>) and RNA polymerase II (<i>RPB2</i>) DNA sequences. The MLST nomenclature for clade 3 of the FSSC assigns numbers to species types (e.g., FSSC 2) and lowercase letters to identify unique haplotypes. The aim of this study was to analyse the genotypic and phenotypic characteristics of 15 environmental and 15 clinical FSSC isolates from Malaysia. MLST was used for the genotypic characterisation of FSSC isolates from various locations within Malaysia, which was complemented by their morphological characterisation on potato dextrose and carnation leaf agar. MLST identified eight different FSSC species: thirteen <i>Fusarium keratoplasticum</i> (i.e., FSSC 2), six <i>Fusarium suttonianum</i> (FSSC 20), five <i>Fusarium falciforme</i> (FSSC 3+4), two <i>Fusarium cyanescens</i> (FSSC 27)<i>,</i> and one each of <i>Fusarium petroliphilum</i> (FSSC 1), <i>Fusarium waltergamsii</i> (FSSC 7), <i>Fusarium</i> sp. (FSSC 12), and <i>Fusarium striatum</i> (FSSC 21). Consistent with previous reports from Malaysia, most (11 of 15) clinical FSSC isolates were <i>F. keratoplasticum</i> and the majority (9 of 15) of environmental isolates were <i>F. suttonianum</i> (5) or <i>F. falciforme</i> (4) strains. The taxonomic relationships of the isolates were resolved phylogenetically. The eight <i>Fusarium</i> species also showed distinct morphological characteristics, but these were less clearly defined and reached across species boundaries. Although <i>TEF1-α</i> and <i>RPB2</i> sequences were sufficient for the species identification of most FSSC isolates, a more precise MLST scheme needs to be established to reliably assign individual isolates of the species-rich FSSC to their geographically-, epidemiologically-, and host-associated sub-lineages. |
| first_indexed | 2024-03-09T13:08:35Z |
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| institution | Directory Open Access Journal |
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| last_indexed | 2024-03-09T13:08:35Z |
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| spelling | doaj.art-5088301608c9457b8da0f5facdd46e132023-11-30T21:45:06ZengMDPI AGJournal of Fungi2309-608X2022-08-018884510.3390/jof8080845Morphology, Phenotype, and Molecular Identification of Clinical and Environmental <i>Fusarium solani</i> Species Complex Isolates from MalaysiaJasper E. James0Jacinta Santhanam1Latiffah Zakaria2Nuraini Mamat Rusli3Mariahyati Abu Bakar4Satinee Suetrong5Jariya Sakayaroj6Mohd Fuat Abdul Razak7Erwin Lamping8Richard D. Cannon9Biomedical Science Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, MalaysiaBiomedical Science Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, Gelugor 11800, MalaysiaBiomedical Science Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, MalaysiaBiomedical Science Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur 50300, MalaysiaNational Center for Genetic Engineering and Biotechnology (BIOTEC), Thailand Science Park, Pathum Thani 12120, ThailandSchool of Science, Wailalak University, Nakhonsithammarat 80161, ThailandBacteriology Unit, Institute for Medical Research, National Institute of Health, Shah Alam 40170, MalaysiaSir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin 9016, New ZealandSir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin 9016, New Zealand<i>Fusarium</i> infections in humans (fusariosis) and in economically important plants involve species of several <i>Fusarium</i> species complexes. Species of the <i>Fusarium solani</i> species complex (FSSC) are the most frequent cause of human fusariosis. The FSSC comprises more than 60 closely related species that can be separated into three major clades by multi-locus sequence typing (MLST) using translation elongation factor 1-alpha (<i>TEF1-α</i>) and RNA polymerase II (<i>RPB2</i>) DNA sequences. The MLST nomenclature for clade 3 of the FSSC assigns numbers to species types (e.g., FSSC 2) and lowercase letters to identify unique haplotypes. The aim of this study was to analyse the genotypic and phenotypic characteristics of 15 environmental and 15 clinical FSSC isolates from Malaysia. MLST was used for the genotypic characterisation of FSSC isolates from various locations within Malaysia, which was complemented by their morphological characterisation on potato dextrose and carnation leaf agar. MLST identified eight different FSSC species: thirteen <i>Fusarium keratoplasticum</i> (i.e., FSSC 2), six <i>Fusarium suttonianum</i> (FSSC 20), five <i>Fusarium falciforme</i> (FSSC 3+4), two <i>Fusarium cyanescens</i> (FSSC 27)<i>,</i> and one each of <i>Fusarium petroliphilum</i> (FSSC 1), <i>Fusarium waltergamsii</i> (FSSC 7), <i>Fusarium</i> sp. (FSSC 12), and <i>Fusarium striatum</i> (FSSC 21). Consistent with previous reports from Malaysia, most (11 of 15) clinical FSSC isolates were <i>F. keratoplasticum</i> and the majority (9 of 15) of environmental isolates were <i>F. suttonianum</i> (5) or <i>F. falciforme</i> (4) strains. The taxonomic relationships of the isolates were resolved phylogenetically. The eight <i>Fusarium</i> species also showed distinct morphological characteristics, but these were less clearly defined and reached across species boundaries. Although <i>TEF1-α</i> and <i>RPB2</i> sequences were sufficient for the species identification of most FSSC isolates, a more precise MLST scheme needs to be established to reliably assign individual isolates of the species-rich FSSC to their geographically-, epidemiologically-, and host-associated sub-lineages.https://www.mdpi.com/2309-608X/8/8/845<i>Fusarium solani</i>FSSCMLST<i>TEF1-α</i><i>RPB2</i> |
| spellingShingle | Jasper E. James Jacinta Santhanam Latiffah Zakaria Nuraini Mamat Rusli Mariahyati Abu Bakar Satinee Suetrong Jariya Sakayaroj Mohd Fuat Abdul Razak Erwin Lamping Richard D. Cannon Morphology, Phenotype, and Molecular Identification of Clinical and Environmental <i>Fusarium solani</i> Species Complex Isolates from Malaysia Journal of Fungi <i>Fusarium solani</i> FSSC MLST <i>TEF1-α</i> <i>RPB2</i> |
| title | Morphology, Phenotype, and Molecular Identification of Clinical and Environmental <i>Fusarium solani</i> Species Complex Isolates from Malaysia |
| title_full | Morphology, Phenotype, and Molecular Identification of Clinical and Environmental <i>Fusarium solani</i> Species Complex Isolates from Malaysia |
| title_fullStr | Morphology, Phenotype, and Molecular Identification of Clinical and Environmental <i>Fusarium solani</i> Species Complex Isolates from Malaysia |
| title_full_unstemmed | Morphology, Phenotype, and Molecular Identification of Clinical and Environmental <i>Fusarium solani</i> Species Complex Isolates from Malaysia |
| title_short | Morphology, Phenotype, and Molecular Identification of Clinical and Environmental <i>Fusarium solani</i> Species Complex Isolates from Malaysia |
| title_sort | morphology phenotype and molecular identification of clinical and environmental i fusarium solani i species complex isolates from malaysia |
| topic | <i>Fusarium solani</i> FSSC MLST <i>TEF1-α</i> <i>RPB2</i> |
| url | https://www.mdpi.com/2309-608X/8/8/845 |
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