Antibacterial Effect of Allicin, Silver Nano Particles, and Their Combination against Skin Infection due to Pseudomonas aeruginosa in Animal Model
Background & Objective: Common antibiotics may be useless against multiple pathogens because of the multidrug resistance. Therefore, new ways of nanotechnology and effective herbal combination can be the solution for this problem. The objective of this study is to investigate the antimicrobial e...
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| Format: | Article |
| Language: | fas |
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Fasa University of Medical Sciences
2014-11-01
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| Series: | Journal of Fasa University of Medical Sciences |
| Subjects: | |
| Online Access: | http://journal.fums.ac.ir/browse.php?a_code=A-10-53-1&slc_lang=en&sid=1 |
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| author | Fatemeh Mirzaei Mojtaba Salouti Reza Shapouri Hamed Alizadeh |
| author_facet | Fatemeh Mirzaei Mojtaba Salouti Reza Shapouri Hamed Alizadeh |
| author_sort | Fatemeh Mirzaei |
| collection | DOAJ |
| description | Background & Objective: Common antibiotics may be useless against multiple pathogens
because of the multidrug resistance. Therefore, new ways of nanotechnology and
effective herbal combination can be the solution for this problem. The
objective of this study is to investigate the antimicrobial effect of Allicin,
silver nano particles and their combination against skin infection due to Pseudomonas
aeruginosa in animal model.Materials & Methods:
In this experimental
study, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal
Concentration (MBC) of Allicin, silver nano particles, and their combination
were determined by macro dilution susceptibility test. Skin infection was
produced in the shoulder region of 12 mice using Pseudomonas aeruginosa and the
effect of ointment of Allicin, silver nano particles, and their combination
were evaluated. Results: The results showed that MIC and MBC of Allicin
for pseudomonas aeruginosa were 2.38 and 4.77 mg/ml, respectively. MIC and MBC
of silver nano particle for Pseudomonas aeruginosa were 3.12 and 6.25 ppm,
respectively. MIC and MBC of combination of Allicin and silver nano particles
on Pseudomonas aeruginosa were 0.59 mg/ml and 1.5 ppm, 1.19 mg/ml and 3.12 ppm,
respectively. Anti-microbial effect of Allicin, silver nano particles and the
synergistic activity of their combination against skin infections due to
Pseudomonas aeruginosa was observed in animal model.Conclusion: The results showed that Allicin in combination
with silver nano particles have synergistic effect against skin infection due
to Pseudomonas aeruginosa. |
| first_indexed | 2024-12-18T15:21:19Z |
| format | Article |
| id | doaj.art-508c393f3de84d5587919f59f8eec6f6 |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2228-7329 |
| language | fas |
| last_indexed | 2024-12-18T15:21:19Z |
| publishDate | 2014-11-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Fasa University of Medical Sciences |
| spelling | doaj.art-508c393f3de84d5587919f59f8eec6f62022-12-21T21:03:23ZfasFasa University of Medical SciencesJournal of Fasa University of Medical Sciences2228-51052228-73292014-11-0143311317Antibacterial Effect of Allicin, Silver Nano Particles, and Their Combination against Skin Infection due to Pseudomonas aeruginosa in Animal ModelFatemeh Mirzaei0Mojtaba Salouti 1Reza Shapouri 2Hamed Alizadeh 3 Department of Microbiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran. Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran. Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran. Young Researchers and Elite Club, Zanjan Branch, Islamic Azad University, Zanjan, Iran. Background & Objective: Common antibiotics may be useless against multiple pathogens because of the multidrug resistance. Therefore, new ways of nanotechnology and effective herbal combination can be the solution for this problem. The objective of this study is to investigate the antimicrobial effect of Allicin, silver nano particles and their combination against skin infection due to Pseudomonas aeruginosa in animal model.Materials & Methods: In this experimental study, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Allicin, silver nano particles, and their combination were determined by macro dilution susceptibility test. Skin infection was produced in the shoulder region of 12 mice using Pseudomonas aeruginosa and the effect of ointment of Allicin, silver nano particles, and their combination were evaluated. Results: The results showed that MIC and MBC of Allicin for pseudomonas aeruginosa were 2.38 and 4.77 mg/ml, respectively. MIC and MBC of silver nano particle for Pseudomonas aeruginosa were 3.12 and 6.25 ppm, respectively. MIC and MBC of combination of Allicin and silver nano particles on Pseudomonas aeruginosa were 0.59 mg/ml and 1.5 ppm, 1.19 mg/ml and 3.12 ppm, respectively. Anti-microbial effect of Allicin, silver nano particles and the synergistic activity of their combination against skin infections due to Pseudomonas aeruginosa was observed in animal model.Conclusion: The results showed that Allicin in combination with silver nano particles have synergistic effect against skin infection due to Pseudomonas aeruginosa.http://journal.fums.ac.ir/browse.php?a_code=A-10-53-1&slc_lang=en&sid=1Skin Infection Pseudomonas aeruginosa Allicin silver Nanoparticles Synergistic Effect |
| spellingShingle | Fatemeh Mirzaei Mojtaba Salouti Reza Shapouri Hamed Alizadeh Antibacterial Effect of Allicin, Silver Nano Particles, and Their Combination against Skin Infection due to Pseudomonas aeruginosa in Animal Model Journal of Fasa University of Medical Sciences Skin Infection Pseudomonas aeruginosa Allicin silver Nanoparticles Synergistic Effect |
| title | Antibacterial Effect of Allicin, Silver Nano Particles, and Their Combination against Skin Infection due to Pseudomonas aeruginosa in Animal Model |
| title_full | Antibacterial Effect of Allicin, Silver Nano Particles, and Their Combination against Skin Infection due to Pseudomonas aeruginosa in Animal Model |
| title_fullStr | Antibacterial Effect of Allicin, Silver Nano Particles, and Their Combination against Skin Infection due to Pseudomonas aeruginosa in Animal Model |
| title_full_unstemmed | Antibacterial Effect of Allicin, Silver Nano Particles, and Their Combination against Skin Infection due to Pseudomonas aeruginosa in Animal Model |
| title_short | Antibacterial Effect of Allicin, Silver Nano Particles, and Their Combination against Skin Infection due to Pseudomonas aeruginosa in Animal Model |
| title_sort | antibacterial effect of allicin silver nano particles and their combination against skin infection due to pseudomonas aeruginosa in animal model |
| topic | Skin Infection Pseudomonas aeruginosa Allicin silver Nanoparticles Synergistic Effect |
| url | http://journal.fums.ac.ir/browse.php?a_code=A-10-53-1&slc_lang=en&sid=1 |
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