Evaluation of a Most Probable Number Method for Detection and Quantification of <i>Legionella pneumophila</i>
The detection and enumeration of <i>Legionella pneumophila</i> (<i>L. pneumophila</i>) in water is crucial for water quality management, human health and has been a research hotspot worldwide. Due to the time-consuming and complicated operation of the plate culture method, it...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/7/789 |
| _version_ | 1797433175932141568 |
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| author | Chunyan Niu Yajie Zhang Yong Zhang |
| author_facet | Chunyan Niu Yajie Zhang Yong Zhang |
| author_sort | Chunyan Niu |
| collection | DOAJ |
| description | The detection and enumeration of <i>Legionella pneumophila</i> (<i>L. pneumophila</i>) in water is crucial for water quality management, human health and has been a research hotspot worldwide. Due to the time-consuming and complicated operation of the plate culture method, it is necessary to adopt a fast and effective method for application. The present study aimed to comprehensively evaluate the performance and applicability of the MPN method by comparing its qualitative and quantitative results with the GB/T 18204.5-2013 and ISO methods, respectively. The qualitative results showed that 372 samples (53%) were negative for both methods; 315 samples (45%) were positively determined by the MPN method, compared with 211 samples (30%) using GB/T 18204.5-2013. The difference in the detection rate between the two methods was statistically significant. In addition, the quantitative results showed that the concentration of <i>L. pneumophila</i> by the MPN method was greater than ISO 11731 and the difference was statistically significant. However, the two methods were different but highly correlated (r = 0.965, <i>p</i> < 0.001). The specificity and sensitivity of the MPN method were 89.85% and 95.73%, respectively. Overall, the results demonstrated that the MPN method has higher sensitivity, a simple operation process and good application prospects in the routine monitoring of <i>L. pneumophila</i> from water samples. |
| first_indexed | 2024-03-09T10:13:19Z |
| format | Article |
| id | doaj.art-509260cef7024d2f87af334120a5be9b |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T10:13:19Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-509260cef7024d2f87af334120a5be9b2023-12-01T22:32:51ZengMDPI AGPathogens2076-08172022-07-0111778910.3390/pathogens11070789Evaluation of a Most Probable Number Method for Detection and Quantification of <i>Legionella pneumophila</i>Chunyan Niu0Yajie Zhang1Yong Zhang2Institute for Environmental Health, Beijing Center for Disease Prevention and Control, Beijing 100013, ChinaInstitute for Environmental Health, Beijing Center for Disease Prevention and Control, Beijing 100013, ChinaInstitute for Environmental Health, Beijing Center for Disease Prevention and Control, Beijing 100013, ChinaThe detection and enumeration of <i>Legionella pneumophila</i> (<i>L. pneumophila</i>) in water is crucial for water quality management, human health and has been a research hotspot worldwide. Due to the time-consuming and complicated operation of the plate culture method, it is necessary to adopt a fast and effective method for application. The present study aimed to comprehensively evaluate the performance and applicability of the MPN method by comparing its qualitative and quantitative results with the GB/T 18204.5-2013 and ISO methods, respectively. The qualitative results showed that 372 samples (53%) were negative for both methods; 315 samples (45%) were positively determined by the MPN method, compared with 211 samples (30%) using GB/T 18204.5-2013. The difference in the detection rate between the two methods was statistically significant. In addition, the quantitative results showed that the concentration of <i>L. pneumophila</i> by the MPN method was greater than ISO 11731 and the difference was statistically significant. However, the two methods were different but highly correlated (r = 0.965, <i>p</i> < 0.001). The specificity and sensitivity of the MPN method were 89.85% and 95.73%, respectively. Overall, the results demonstrated that the MPN method has higher sensitivity, a simple operation process and good application prospects in the routine monitoring of <i>L. pneumophila</i> from water samples.https://www.mdpi.com/2076-0817/11/7/789MPN methodplate culture<i>Legionella pneumophila</i>cooling watercondensed watersanitary hot water |
| spellingShingle | Chunyan Niu Yajie Zhang Yong Zhang Evaluation of a Most Probable Number Method for Detection and Quantification of <i>Legionella pneumophila</i> Pathogens MPN method plate culture <i>Legionella pneumophila</i> cooling water condensed water sanitary hot water |
| title | Evaluation of a Most Probable Number Method for Detection and Quantification of <i>Legionella pneumophila</i> |
| title_full | Evaluation of a Most Probable Number Method for Detection and Quantification of <i>Legionella pneumophila</i> |
| title_fullStr | Evaluation of a Most Probable Number Method for Detection and Quantification of <i>Legionella pneumophila</i> |
| title_full_unstemmed | Evaluation of a Most Probable Number Method for Detection and Quantification of <i>Legionella pneumophila</i> |
| title_short | Evaluation of a Most Probable Number Method for Detection and Quantification of <i>Legionella pneumophila</i> |
| title_sort | evaluation of a most probable number method for detection and quantification of i legionella pneumophila i |
| topic | MPN method plate culture <i>Legionella pneumophila</i> cooling water condensed water sanitary hot water |
| url | https://www.mdpi.com/2076-0817/11/7/789 |
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